© Thomas Zobel

Microscopy seminars

© Ali Gheisari - CMCB light microscopy facility/TU Dresden

NEW: FLIM videos on Youtube

Find a collection of videos recorded during the symposium “Beginners guide to fluorescence lifetime imaging microscopy (FLIM)” last year. The playlist includes more than 20 hours of introductory lectures and interactive demonstrations at the microscope. The symposium was organised by our Imaging Network together with many partners.

Seminar series

Every two weeks on Fridays, we provide an introduction into different instruments and methods for fluorescence microscopy – starting with the preparation of samples as well as basic functions and instrument settings up to characteristics in the technical design of special microscopes. The techniques covered include fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET) and fluorescence-lifetime imaging microscopy (FLIM), super-resolution methods such as stimulated emission depletion (STED) and single molecule localization microscopy (SMLM), and single particle tracking.

Everyone working at Münster University, and especially doctoral students, are welcome to participate! The seminar series is organised by the Imaging Network of the University of Münster.

Topics and dates

On Fridays, 12:00 to 12:45 p.m.
Speaker: Thomas Zobel, Coordinator Microscopy – Imaging Network

Corona – current note: Our meetings are currently held online via Zoom. Please request the login data early enough for the sessions you would like to attend (via cim@uni-muenster.de indicating your name and institution).

Date, time and place Topics
no lectures at present

  • Previous topics and dates

    Date, time and place Topics
    12.03.2021
    12:00-12:45
    Online seminar    		 RDM4Mic
    Research data management for microscopy, OMERO, data storage, analysis server
    26.02.2021
    12:00-12:45
    Online seminar    		 Super-resolution microscopy II
    Single molecule localization microscopy (SMLM, e.g. STORM, PALM, DNA-PAINT), single particle tracking
    12.02.2021
    12:00-12:45
    Online seminar    		 Super-resolution microscopy I
    SIM, STED, MINFLUX, sample preparation
    29.01.2021
    12:00-12:45
    Online seminar    		 Advanced fluorescence microscopy II
    Deconvolution (Huygens), Airyscan (Zeiss), Lightning (Leica), deep learning approaches (N2V / CARE)
    15.01.2021
    12:00-12:45
    Online seminar    		 Advanced fluorescence microscopy I
    FRAP, FRET, FLIM, spectral unmixing
    04.12.2020
    12:00-12:45
    Online seminar    		 Basics of fluorescence microscopy II
    Confocal laser scanning microscopes, beampath, detectors (PMT, HyD, GaAsp), signal to noise ratio, tips for live cell imaging, sample preparation
    06.11.2020
    12:00-12:45
    Online seminar    		 Basics of fluorescence microscopy I
    Wild field systems, point spread function, numerical aperture of an objective, resolution (raylight, FWHM, Abbe diffraction limit), different kinds of fluorophores
    23.10.2020
    12:00-12:45
    Online seminar    		 Microscopy – what’s at hand at Münster University?
    Different microscopy systems, services (OMERO/analysis server), image analysis, provided software, examples of methods from different groups