© Thomas Zobel

Microscopy seminars

This seminar series provides an introduction into different instruments and methods for fluorescence microscopy – starting with the preparation of samples as well as basic functions and instrument settings up to characteristics in the technical design of special microscopes. The techniques covered include fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET) and fluorescence-lifetime imaging microscopy (FLIM), super-resolution methods such as stimulated emission depletion (STED) and single molecule localization microscopy (SMLM), and single particle tracking.

Everyone working at Münster University, and especially doctoral students, are welcome to participate! The seminar series is organised by the Imaging Network of the University of Münster.

Topics and dates

On Fridays, 12:00 to 12:45 p.m.
Speaker:
Thomas Zobel, Coordinator Microscopy – Imaging Network

Corona – current note: Our meetings are currently held online via Zoom. Please request the login data early enough for the sessions you would like to attend (via cim@uni-muenster.de indicating your name and institution).

Date, time and place Topics
06.11.2020
12:00-12:45
Online seminar
Basics of fluorescence microscopy I
Wild field systems, point spread function, numerical aperture of an objective, resolution (raylight, FWHM, Abbe diffraction limit), different kinds of fluorophores
20.11.2020
12:00-12:45
Online seminar
Basics of fluorescence microscopy II
Confocal laser scanning microscopes, beampath, detectors (PMT, HyD, GaAsp), signal to noise ratio, tips for live cell imaging, sample preparation
04.12.2020
12:00-12:45
Online seminar
Advanced fluorescence microscopy I
FRAP, FRET, FLIM, spectral unmixing
15.01.2021
12:00-12:45
Online seminar
Advanced fluorescence microscopy II
Deconvolution (Huygens), Airyscan (Zeiss), Lightning (Leica), deep learning approaches (N2V / CARE)
29.01.2021
12:00-12:45
Online seminar
Super-resolution microscopy I
SIM, STED, MINFLUX, sample preparation
12.02.2021
12:00-12:45
Online seminar
Super-resolution microscopy II
Single molecule localization microscopy (SMLM, e.g. STORM, PALM, DNA-PAINT), single particle tracking
26.02.2021
12:00-12:45
Online seminar
RDM4Mic
Research data management for microscopy, OMERO, data storage, analysis server

  • Previous topics and dates

    Date, time and place Topics
    23.10.2020
    12:00-12:45
    Online seminar
    Microscopy – what’s at hand at Münster University?
    Different microscopy systems, services (OMERO/analysis server), image analysis, provided software, examples of methods from different groups