NEW: FLIM videos on Youtube
Seminar series
Every two weeks on Fridays, we provide an introduction into different instruments and methods for fluorescence microscopy – starting with the preparation of samples as well as basic functions and instrument settings up to characteristics in the technical design of special microscopes. The techniques covered include fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET) and fluorescence-lifetime imaging microscopy (FLIM), super-resolution methods such as stimulated emission depletion (STED) and single molecule localization microscopy (SMLM), and single particle tracking.
Everyone working at Münster University, and especially doctoral students, are welcome to participate! The seminar series is organised by the Imaging Network of the University of Münster.
Topics and dates
On Fridays, 12:00 to 12:45 p.m.
Speaker: Thomas Zobel, Coordinator Microscopy – Imaging Network
Corona – current note: Our meetings are currently held online via Zoom. Please request the login data early enough for the sessions you would like to attend (via cim@uni-muenster.de indicating your name and institution).
Date, time and place | Topics |
---|---|
12.03.2021 12:00-12:45 Online seminar |
RDM4Mic Research data management for microscopy, OMERO, data storage, analysis server |
Previous topics and dates
Date, time and place Topics 26.02.2021
12:00-12:45
Online seminarSuper-resolution microscopy II
Single molecule localization microscopy (SMLM, e.g. STORM, PALM, DNA-PAINT), single particle tracking12.02.2021
12:00-12:45
Online seminarSuper-resolution microscopy I
SIM, STED, MINFLUX, sample preparation29.01.2021
12:00-12:45
Online seminarAdvanced fluorescence microscopy II
Deconvolution (Huygens), Airyscan (Zeiss), Lightning (Leica), deep learning approaches (N2V / CARE)15.01.2021
12:00-12:45
Online seminarAdvanced fluorescence microscopy I
FRAP, FRET, FLIM, spectral unmixing04.12.2020
12:00-12:45
Online seminarBasics of fluorescence microscopy II
Confocal laser scanning microscopes, beampath, detectors (PMT, HyD, GaAsp), signal to noise ratio, tips for live cell imaging, sample preparation06.11.2020
12:00-12:45
Online seminarBasics of fluorescence microscopy I
Wild field systems, point spread function, numerical aperture of an objective, resolution (raylight, FWHM, Abbe diffraction limit), different kinds of fluorophores23.10.2020
12:00-12:45
Online seminarMicroscopy – what’s at hand at Münster University?
Different microscopy systems, services (OMERO/analysis server), image analysis, provided software, examples of methods from different groups