Every two weeks on Fridays, we provide an introduction into different instruments and methods for fluorescence microscopy – starting with the preparation of samples as well as basic functions and instrument settings up to characteristics in the technical design of special microscopes. The techniques covered include fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET) and fluorescence-lifetime imaging microscopy (FLIM), super-resolution methods such as stimulated emission depletion (STED) and single molecule localization microscopy (SMLM), and single particle tracking.
Everyone working at Münster University, and especially doctoral students, are welcome to participate! The seminar series is organised by the Imaging Network of the University of Münster.
Topics and dates
On Fridays, 12:00 to 12:45 p.m.
Speaker: Thomas Zobel, Coordinator Microscopy – Imaging Network
Corona – current note: Our meetings are currently held online via Zoom. Please request the login data early enough for the sessions you would like to attend (via firstname.lastname@example.org indicating your name and institution).
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Previous topics and dates
Date, time and place Topics 12.03.2021
Research data management for microscopy, OMERO, data storage, analysis server
Super-resolution microscopy II
Single molecule localization microscopy (SMLM, e.g. STORM, PALM, DNA-PAINT), single particle tracking
Super-resolution microscopy I
SIM, STED, MINFLUX, sample preparation
Advanced fluorescence microscopy II
Deconvolution (Huygens), Airyscan (Zeiss), Lightning (Leica), deep learning approaches (N2V / CARE)
Advanced fluorescence microscopy I
FRAP, FRET, FLIM, spectral unmixing
Basics of fluorescence microscopy II
Confocal laser scanning microscopes, beampath, detectors (PMT, HyD, GaAsp), signal to noise ratio, tips for live cell imaging, sample preparation
Basics of fluorescence microscopy I
Wild field systems, point spread function, numerical aperture of an objective, resolution (raylight, FWHM, Abbe diffraction limit), different kinds of fluorophores
Microscopy – what’s at hand at Münster University?
Different microscopy systems, services (OMERO/analysis server), image analysis, provided software, examples of methods from different groups