Collage aus Mikroskopiebildern
© Thomas Zobel

Mikroskopie-Seminare

Seminarreihe „Fluoreszenzmikroskopie“

Wir geben eine Einführung in unterschiedliche Geräte und Methoden für die Fluoreszenzmikroskopie – angefangen bei der Vorbereitung von Proben sowie grundlegenden Funktionen und Geräteeinstellungen bis zu Besonderheiten im technischen Aufbau spezieller Mikroskope. Zu den behandelten Techniken gehören fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET) und fluorescence-lifetime imaging microscopy (FLIM), Super-Resolution-Methoden wie stimulated emission depletion (STED) und single molecule localization microscopy (SMLM) sowie single particle tracking. Alle interessierten Mitarbeitenden der WWU und insbesondere Masterstudierende und Promovierende sind herzlich willkommen!

Themen und Termine

Donnerstags von 12:00 bis 13:00 Uhr
Referent:
Thomas Zobel, Koordinator Mikroskopie – Imaging Network

Unsere Meetings finden zurzeit online über Zoom statt. Die untenstehenden Zugangsdaten sind für alle Veranstaltungen der Vortragsserie gültig.

https://wwu.zoom.us/j/69977678482?pwd=eHgwdmNacFI3bzV0RU1IUGU2TkNXQT09
Meeting-ID: 699 7767 8482
Kenncode: 145159

Datum Themen
19.05.2022 Super-resolution microscopy II
Single molecule localization microscopy (SMLM, e.g. STORM, PALM, DNA-PAINT), single particle tracking
  • Vergangene Themen und Termine

    Datum, Zeit und Ort Themen
    12.05.2022
    12:00-13:00
    Online seminar
    Super-resolution microscopy I
    SIM, STED, MINFLUX, sample preparation
    05.05.2022
    12:00-13:00
    Online seminar
    Advanced fluorescence microscopy II
    Deconvolution (Huygens), Airyscan (Zeiss), Lightning (Leica), deep learning approaches (N2V / CARE)

    07.04.2022
    12:00-13:00
    Online seminar

    Advanced fluorescence microscopy I
    FRAP, FRET, FLIM, spectral unmixing
    24.03.2022
    12:00-13:00
    Online seminar
    Research data management for microscopy data
    OMERO, data storage, analysis server
    17.03.2022
    12:00-13:00
    Online seminar
    Basics of fluorescence microscopy II
    Confocal laser scanning microscopes, beampath, detectors (PMT, HyD, GaAsp), signal to noise ratio, tips for live cell imaging, sample preparation
    10.03.2022
    12:00-13:00
    Online seminar
    Basics of fluorescence microscopy I
    Wild field systems, point spread function, numerical aperture of an objective, resolution (raylight, FWHM, Abbe diffraction limit), different kinds of fluorophores
    03.03.2022
    12:00-13:00
    Online seminar
    Münster Imaging Network – Services & Microscopy Systems
    Overview about the services of the Imaging Network and the available microscopy systems (database & data storage, analysis server, image analysis and more)
    12.03.2021
    12:00-12:45
    Online seminar
    RDM4Mic
    Research data management for microscopy, OMERO, data storage, analysis server
    26.02.2021
    12:00-12:45
    Online seminar
    Super-resolution microscopy II
    Single molecule localization microscopy (SMLM, e.g. STORM, PALM, DNA-PAINT), single particle tracking
    12.02.2021
    12:00-12:45
    Online seminar
    Super-resolution microscopy I
    SIM, STED, MINFLUX, sample preparation
    29.01.2021
    12:00-12:45
    Online seminar
    Advanced fluorescence microscopy II
    Deconvolution (Huygens), Airyscan (Zeiss), Lightning (Leica), deep learning approaches (N2V / CARE)
    15.01.2021
    12:00-12:45
    Online seminar
    Advanced fluorescence microscopy I
    FRAP, FRET, FLIM, spectral unmixing
    04.12.2020
    12:00-12:45
    Online seminar
    Basics of fluorescence microscopy II
    Confocal laser scanning microscopes, beampath, detectors (PMT, HyD, GaAsp), signal to noise ratio, tips for live cell imaging, sample preparation
    06.11.2020
    12:00-12:45
    Online seminar
    Basics of fluorescence microscopy I
    Wild field systems, point spread function, numerical aperture of an objective, resolution (raylight, FWHM, Abbe diffraction limit), different kinds of fluorophores
    23.10.2020
    12:00-12:45
    Online seminar
    Microscopy – what’s at hand at Münster University?
    Different microscopy systems, services (OMERO/analysis server), image analysis, provided software, examples of methods from different groups

     

     

     

Workshops “Image Processing”

Trainer: Thomas Zobel, Koordinator Mikroskopie – Imaging Network

Die Teilnehmerzahl ist auf zehn Personen pro Workshop begrenzt. Bitte senden Sie Ihre Anfrage für die Teilnahme an cim@uni-muenster.de (mit Angabe Ihres Namens und Ihrer Institution).

Die Workshops finden online per Zoom statt. Wir werden Remote auf unserem Analyseserver arbeiten. Idealerweise arbeiten Sie mit einem großen oder zwei Monitoren. Es sind keine weiteren technischen Vorbereitungen notwendig.

Datum, Zeit und Ort Themen
aktuell keine Termine verfügbar  
  • Vergangene Themen und Termine

    Datum, Zeit und Ort Themen
    01.-02.03.2022
    9:00-16:00
    Online workshop
    Image processing with advanced image analysis software & tools

    Topics: overview and short introductions to advanced Fiji plugins, open source software (Ilastik/Cellprofiler) as well as purchased software available at Münster University like Imaris (3D Analysis) and Huygens Professional (deconvolution & general image restoration)

    No prerequisites for participation.

    22.-23.02.2022
    9:00-16:00
    Online workshop

    Image processing with Fiji/ImageJ – Advanced course (macro programming)

    Topics: detailed lecture about ImageJ macro language, step-by-step programming tutorial, open to discuss and write own macros
    Important: Participation in our Fiji/ImageJ beginner course or the Biovoxxel ImageJ/Fiji course, or detailed knowledge of Fiji/first experiences with macros or other programming languages is required.
    15.-16.02.2022
    9:00-16:00
    Online workshop
    Image processing with Fiji/ImageJ – Course for beginners

    For all scientists who work with microscopes and microscopic images! At the end of the course, you will be able to analyse your images in an automated and unbiased way and to prepare your images for scientific figure creation.

    Topics: basic image acquisition and image processing, image histogram and bit depth, image enhancements (background subtraction/using filters for further analysis), image segmentation (e.g. counts of nuclei, creation of regions of interest), (ROI) etc., automated object counting, measurements (e.g. signal quantifications), create figures (split channels, projections, scale bar, annotations), macro recorder and batch processing
    08.-09.02.2022
    9:00-16:00
    Online workshop

    Image processing with Fiji/ImageJ – Course for beginners

    For all scientists who work with microscopes and microscopic images! At the end of the course, you will be able to analyse your images in an automated and unbiased way and to prepare your images for scientific figure creation.

    Topics: basic image acquisition and image processing, image histogram and bit depth, image enhancements (background subtraction/using filters for further analysis), image segmentation (e.g. counts of nuclei, creation of regions of interest), (ROI) etc., automated object counting, measurements (e.g. signal quantifications), create figures (split channels, projections, scale bar, annotations), macro recorder and batch processing

© Ali Gheisari - CMCB light microscopy facility/TU Dresden

FLIM-Videos auf Youtube

Auf Youtube gibt es rund 20 Stunden Videomaterial aus dem Symposium „Beginners guide to fluorescence lifetime imaging microscopy (FLIM)“, das unser Imaging Network im vergangenen Jahr gemeinsam mit zahlreichen Partnern organisiert hat. Die Playlist enthält einführende Vorträge und interaktive Demonstrationen am Mikroskop.