Seminarreihe „Fluoreszenzmikroskopie“
Wir geben eine Einführung in unterschiedliche Geräte und Methoden für die Fluoreszenzmikroskopie – angefangen bei der Vorbereitung von Proben sowie grundlegenden Funktionen und Geräteeinstellungen bis zu Besonderheiten im technischen Aufbau spezieller Mikroskope. Zu den behandelten Techniken gehören fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET) und fluorescence-lifetime imaging microscopy (FLIM), Super-Resolution-Methoden wie stimulated emission depletion (STED) und single molecule localization microscopy (SMLM) sowie single particle tracking. Alle interessierten Mitarbeitenden der WWU und insbesondere Masterstudierende und Promovierende sind herzlich willkommen!
Themen und Termine
Donnerstags von 12:00 bis 13:00 Uhr
Ort: Multiscale Imaging Centre (Auditorium), Röntgenstraße 16
Falls Sie nicht in Präsenz teilnehmen können, gibt es die Möglichkeit, sich per Zoom zuzuschalten.
Meeting-ID: 699 7767 8482
Code: 145159
Referent: Thomas Zobel, Koordinator Mikroskopie – Imaging Network
| Date | Topics |
|---|---|
| 16.03.2023 | RDM4Mic Research data management for microscopy, OMERO, data storage, analysis server |
| 30.03.2023 | Microscopy – what’s at hand at Münster University? Different microscopy systems, services (OMERO/analysis server), image analysis, provided software, examples of methods from different groups |
| 20.04.2023 | Basics of fluorescence microscopy I Wild field systems, point spread function, numerical aperture of an objective, resolution (raylight, FWHM, Abbe diffraction limit), different kinds of fluorophores |
| 27.04.2023 cancelled |
Basics of fluorescence microscopy II Confocal laser scanning microscopes, beampath, detectors (PMT, HyD, GaAsp), signal to noise ratio, tips for live cell imaging, sample preparation |
| 04.05.2023 | Advanced fluorescence microscopy I FRAP, FRET, FLIM, spectral unmixing |
| 25.05.2023 | Advanced fluorescence microscopy II Deconvolution (Huygens), Airyscan (Zeiss), Lightning (Leica), deep learning approaches (N2V / CARE) |
| 22.06.2023 | Super-resolution microscopy I SIM, STED, MINFLUX, sample preparation |
| 06.07.2023 | Super-resolution microscopy II Single molecule localization microscopy (SMLM, e.g. STORM, PALM, DNA-PAINT), single particle tracking |
Vergangene Themen und Termine
Datum, Zeit und Ort Themen 19.05.2022
12:00-13:00
Online seminarSuper-resolution microscopy II
Single molecule localization microscopy (SMLM, e.g. STORM, PALM, DNA-PAINT), single particle tracking12.05.2022
12:00-13:00
Online seminarSuper-resolution microscopy I
SIM, STED, MINFLUX, sample preparation05.05.2022
12:00-13:00
Online seminarAdvanced fluorescence microscopy II
Deconvolution (Huygens), Airyscan (Zeiss), Lightning (Leica), deep learning approaches (N2V / CARE)07.04.2022
12:00-13:00
Online seminarAdvanced fluorescence microscopy I
FRAP, FRET, FLIM, spectral unmixing24.03.2022
12:00-13:00
Online seminarResearch data management for microscopy data
OMERO, data storage, analysis server17.03.2022
12:00-13:00
Online seminarBasics of fluorescence microscopy II
Confocal laser scanning microscopes, beampath, detectors (PMT, HyD, GaAsp), signal to noise ratio, tips for live cell imaging, sample preparation10.03.2022
12:00-13:00
Online seminarBasics of fluorescence microscopy I
Wild field systems, point spread function, numerical aperture of an objective, resolution (raylight, FWHM, Abbe diffraction limit), different kinds of fluorophores03.03.2022
12:00-13:00
Online seminarMünster Imaging Network – Services & Microscopy Systems
Overview about the services of the Imaging Network and the available microscopy systems (database & data storage, analysis server, image analysis and more)12.03.2021
12:00-12:45
Online seminarRDM4Mic
Research data management for microscopy, OMERO, data storage, analysis server26.02.2021
12:00-12:45
Online seminarSuper-resolution microscopy II
Single molecule localization microscopy (SMLM, e.g. STORM, PALM, DNA-PAINT), single particle tracking12.02.2021
12:00-12:45
Online seminarSuper-resolution microscopy I
SIM, STED, MINFLUX, sample preparation29.01.2021
12:00-12:45
Online seminarAdvanced fluorescence microscopy II
Deconvolution (Huygens), Airyscan (Zeiss), Lightning (Leica), deep learning approaches (N2V / CARE)15.01.2021
12:00-12:45
Online seminarAdvanced fluorescence microscopy I
FRAP, FRET, FLIM, spectral unmixing04.12.2020
12:00-12:45
Online seminarBasics of fluorescence microscopy II
Confocal laser scanning microscopes, beampath, detectors (PMT, HyD, GaAsp), signal to noise ratio, tips for live cell imaging, sample preparation06.11.2020
12:00-12:45
Online seminarBasics of fluorescence microscopy I
Wild field systems, point spread function, numerical aperture of an objective, resolution (raylight, FWHM, Abbe diffraction limit), different kinds of fluorophores23.10.2020
12:00-12:45
Online seminarMicroscopy – what’s at hand at Münster University?
Different microscopy systems, services (OMERO/analysis server), image analysis, provided software, examples of methods from different groups
FLIM-Videos auf Youtube
Workshops “Image Processing”
Trainer: Thomas Zobel, Koordinator Mikroskopie – Imaging Network
Die Teilnehmerzahl ist auf zehn Personen pro Workshop begrenzt. Bitte senden Sie Ihre Anfrage für die Teilnahme an cim@uni-muenster.de (mit Angabe Ihres Namens und Ihrer Institution).
Die Workshops finden online per Zoom statt. Wir werden Remote auf unserem Analyseserver arbeiten. Idealerweise arbeiten Sie mit einem großen oder zwei Monitoren. Es sind keine weiteren technischen Vorbereitungen notwendig.
| Datum, Zeit und Ort | Themen |
|---|---|
| aktuell keine Termine verfügbar |
Vergangene Themen und Termine
Datum, Zeit und Ort Themen 01.-02.03.2022
9:00-16:00
Online workshopImage processing with advanced image analysis software & tools
Topics: overview and short introductions to advanced Fiji plugins, open source software (Ilastik/Cellprofiler) as well as purchased software available at Münster University like Imaris (3D Analysis) and Huygens Professional (deconvolution & general image restoration)
No prerequisites for participation.22.-23.02.2022
9:00-16:00
Online workshopImage processing with Fiji/ImageJ – Advanced course (macro programming)
Topics: detailed lecture about ImageJ macro language, step-by-step programming tutorial, open to discuss and write own macros
Important: Participation in our Fiji/ImageJ beginner course or the Biovoxxel ImageJ/Fiji course, or detailed knowledge of Fiji/first experiences with macros or other programming languages is required.15.-16.02.2022
9:00-16:00
Online workshopImage processing with Fiji/ImageJ – Course for beginners
For all scientists who work with microscopes and microscopic images! At the end of the course, you will be able to analyse your images in an automated and unbiased way and to prepare your images for scientific figure creation.
Topics: basic image acquisition and image processing, image histogram and bit depth, image enhancements (background subtraction/using filters for further analysis), image segmentation (e.g. counts of nuclei, creation of regions of interest), (ROI) etc., automated object counting, measurements (e.g. signal quantifications), create figures (split channels, projections, scale bar, annotations), macro recorder and batch processing08.-09.02.2022
9:00-16:00
Online workshopImage processing with Fiji/ImageJ – Course for beginners
For all scientists who work with microscopes and microscopic images! At the end of the course, you will be able to analyse your images in an automated and unbiased way and to prepare your images for scientific figure creation.
Topics: basic image acquisition and image processing, image histogram and bit depth, image enhancements (background subtraction/using filters for further analysis), image segmentation (e.g. counts of nuclei, creation of regions of interest), (ROI) etc., automated object counting, measurements (e.g. signal quantifications), create figures (split channels, projections, scale bar, annotations), macro recorder and batch processing