Dynamic imaging and molecular mechanisms of myeloid cell infiltration into the inflamed peritoneum

Principal investigator: Lydia Sorokin
Project number: CRC 1450 C04
Project term: 01/2021–12/2024

Graphical abstract
© CRC inSight

To define routes and mechanisms of leukocyte infiltration into the peritoneum during peritonitis a multiscale imaging approach will be employed to analyse the temporo-spatial dynamics of the extravasation of different myeloid populations across the high endothelial venule (HEV)-like versus conventional postcapillary venules of the omentum in response to different sterile or infectious stimuli (1, 2).

Combined with single cell RNA sequencing of omental vessels, this will identify marker molecules (3) for the development of non-invasive imaging strategies (4) for assessing acute peritoneal inflammation and, potentially, also for its manipulation.


Principal investigator

Project member


The names of the principal investigators in our network have been bolded. Publications released prior to 2021, when funding for our network commenced, represent previous project-related work.


Zhang X, Wang Y, Song J, Gerwien H, Chuquisana O, Chashchina A, Denz C, Sorokin L. The endothelial basement membrane acts as a checkpoint for entry of pathogenic T cells into the brain. J Exp Med 2020;217: e20191339. Abstract


Di Russo J, Luik A-L, Yousif L, Budny S, Oberleithner H, Hofschröer V, Klingauf J, van Bavel E, Bakker E NTP, Hellstrand P, Bhattachariya A, Albinsson S, Pincet F, Hallmann R, Sorokin L. Endothelial basement membrane laminin 511 is essential for shear stress response. EMBO J 2017;36: 183-201. Abstract
Song J, Zhang X, Buscher K, Wang Y, Wang H, Di Russo J, Li L, Lutke-Enking S, Zarbock A, Stadtmann A, Striewski P, Wirth B, Kuzmanov I, Wiendl H, Schulte D, Vestweber D, Sorokin L. Endothelial Basement Membrane Laminin 511 Contributes to Endothelial Junctional Tightness and Thereby Inhibits Leukocyte Transmigration. Cell Rep 2017;18: 1256-1269. Abstract


Buscher K, Wang H, Zhang X, Striewski P, Wirth B, Saggu G, Lutke-Enking S, Mayadas TN, Ley K, Sorokin L, Song J. Protection from septic peritonitis by rapid neutrophil recruitment through omental high endothelial venules. Nat Commun 2016;7: 10828. Abstract
Gerwien H, Hermann S, Zhang X, Korpos E, Song J, Kopka K, Faust A, Wenning C, Gross CC, Honold L, Melzer N, Opdenakker G, Wiendl H, Schafers M, Sorokin L. Imaging matrix metalloproteinase activity in multiple sclerosis as a specific marker of leukocyte penetration of the blood-brain barrier. Sci Transl Med 2016;8: 364ra152. Abstract


Song J, Lokmic Z, Lämmermann T, Rolf J, Wu C, Zhang X, Hallmann R, Hannocks M-J, Horn N, Ruegg MA, Sonnenberg A, Georges-Labouesse E, Winkler TH, Kearney JF, Cardell S, Sorokin L. Extracellular matrix of secondary lymphoid organs impacts on B-cell fate and survival. Proc Natl Acad Sci USA 2013;110: E2915-24. Abstract


Schumann K, Lammermann T, Bruckner M, Legler DF, Polleux J, Spatz JP, Schuler G, Forster R, Lutz MB, Sorokin L, Sixt M. Immobilized chemokine fields and soluble chemokine gradients cooperatively shape migration patterns of dendritic cells. Immunity 2010;32: 703-713. Abstract


Wu C, Ivars F, Anderson P, Hallmann R, Vestweber D, Nilsson P, Robenek H, Tryggvason K, Song J, Korpos E, Loser K, Beissert S, Georges-Labouesse E, Sorokin LM. Endothelial basement membrane laminin alpha5 selectively inhibits T lymphocyte extravasation into the brain. Nat Med 2009;15: 519-527. Abstract


Sixt M, Kanazawa N, Selg M, Samson T, Roos G, Reinhardt DP, Pabst R, Lutz MB, Sorokin LM. The conduit system transports soluble antigens from the afferent lymph to resident dendritic cells in the T cell area of the lymph node. Immunity 2005;22: 19-29. Abstract