Metabolic priming of monocytes during inflammation in vivo
Principal investigators: Johannes Roth, Michael Schäfers
Project number: CRC 1450 C01
Project term: 01/2021–12/2024

Monocytes represent a heterogeneous population, which, depending on their stage of differentiation and activation, either can promote inflammation or induce resolution and repair. Time and duration of initial exogenous or endogenous triggers determine such subsequent pro- or anti-inflammatory monocyte response patterns in vitro partially through shifts between glucose and lipid metabolism (metabolic priming) (1). However, occurrence and relevance of these regulatory mechanisms in vivo is less clear.
We aim at monitoring metabolism and spatio-temporal dynamics of monocytes (2, 3, 4) and their functional contribution to inflammation in vivo through tracking of monocytes and dynamic metabolic imaging in murine models of autoimmunity-, hypoxia- and infection-driven inflammation (5).
Team
Principal investigators
- Roth, Johannes, Univ.-Prof. Dr. med.
Institute of Immunology, WWU - Schäfers, Michael, Univ.-Prof. Dr. med.
European Institute for Molecular Imaging, WWU & Department of Nuclear Medicine, UKM
Project members
- Jürgens, Ricarda
Institute of Immunology, WWU - Heiden, Nadine
Department of Nuclear Medicine, UKM
European Institute for Molecular Imaging, WWU - Jansen, Lea
European Institute for Molecular Imaging, WWU
Publications
The names of the principal investigators in our network have been bolded. Publications released prior to 2021, when funding for our network commenced, represent previous project-related work.
2021
Depke DA, Konken CP, Rosner L, Hermann S, Schafers M, Rentmeister A. A novel (18)F-labeled clickable substrate for targeted imaging of SNAP-tag expressing cells by PET in vivo. Chem Commun (Camb) 2021;57: 9850-9853. Abstract |
Honold L, Austrup M, Faust A, Konken CP, Schwegmann K, Zinnhardt B, Daniliuc CG, Haufe G, Schafers M, Kopka K, Hermann S. Towards Optimized Bioavailability of (99m)Tc-Labeled Barbiturates for Non-invasive Imaging of Matrix Metalloproteinase Activity. Mol Imaging Biol 2021Abstract |
2020
Dittmann M, Gonzalez Carvalho JM, Rahbar K, Schafers M, Claesener M, Riemann B, Seifert R. Incremental diagnostic value of [(18)F]tetrafluoroborate PET-CT compared to [(131)I]iodine scintigraphy in recurrent differentiated thyroid cancer. Eur J Nucl Med Mol Imaging 2020;47: 2639-2646. Abstract |
Fischer-Riepe L, Daber N, Schulte-Schrepping J, Veras De Carvalho BC, Russo A, Pohlen M, Fischer J, Chasan AI, Wolf M, Ulas T, Glander S, Schulz C, Skryabin B, Wollbrink Dipl-Ing A, Steingraeber N, Stremmel C, Koehle M, Gartner F, Vettorazzi S, Holzinger D, Gross J, Rosenbauer F, Stoll M, Niemann S, Tuckermann J, Schultze JL, Roth J, Barczyk-Kahlert K. CD163 expression defines specific, IRF8-dependent, immune-modulatory macrophages in the bone marrow. J Allergy Clin Immunol 2020;146: 1137-1151. Abstract |
2019
Fischer J, Walter C, Tonges A, Aleth H, Jordao MJC, Leddin M, Groning V, Erdmann T, Lenz G, Roth J, Vogl T, Prinz M, Dugas M, Jacobsen ID, Rosenbauer F. Safeguard function of PU1 shapes the inflammatory epigenome of neutrophils. Nat Immunol 2019;20: 546-558. Abstract |
Freise N, Burghard A, Ortkras T, Daber N, Imam Chasan A, Jauch S-L, Fehler O, Hillebrand J, Schakaki M, Rojas J, Grimbacher B, Vogl T, Hoffmeier A, Martens S, Roth J, Austermann J. Signaling mechanisms inducing hyporesponsiveness of phagocytes during systemic inflammation. Blood 2019;134: 134-146. Abstract |
2018
Gran S, Honold L, Fehler O, Zenker S, Eligehausen S, Kuhlmann MT, Geven E, den Bosch M van, van Lent P, Spiekermann C, Hermann S, Vogl T, Schäfers M, Roth J. Imaging, myeloid precursor immortalization, and genome editing for defining mechanisms of leukocyte recruitment_in vivo_. Theranostics 2018;8: 2407-2423. Abstract |
Heming M, Gran S, Jauch S-L, Fischer-Riepe L, Russo A, Klotz L, Hermann S, Schafers M, Roth J, Barczyk-Kahlert K. Peroxisome Proliferator-Activated Receptor-γ Modulates the Response of Macrophages to Lipopolysaccharide and Glucocorticoids. Front Immunol 2018;9: 893. Abstract |
Vogl T, Stratis A, Wixler V, Voller T, Thurainayagam S, Jorch SK, Zenker S, Dreiling A, Chakraborty D, Frohling M, Paruzel P, Wehmeyer C, Hermann S, Papantonopoulou O, Geyer C, Loser K, Schafers M, Ludwig S, Stoll M, Leanderson T, Schultze JL, Konig S, Pap T, Roth J. Autoinhibitory regulation of S100A8/S100A9 alarmin activity locally restricts sterile inflammation. J Clin Invest 2018;128: 1852-1866. Abstract |
2017
Thunemann M, Schörg BF, Feil S, Lin Y, Voelkl J, Golla M, Vachaviolos A, Kohlhofer U, Quintanilla-Martinez L, Olbrich M, Ehrlichmann W, Reischl G, Griessinger CM, Langer HF, Gawaz M, Lang F, Schäfers M, Kneilling M, Pichler BJ, Feil R. Cre/lox-assisted non-invasive in vivo tracking of specific cell populations by positron emission tomography. Nat Commun 2017;8: 444. Abstract |
Ulas T, Pirr S, Fehlhaber B, Bickes MS, Loof TG, Vogl T, Mellinger L, Heinemann AS, Burgmann J, Schoning J, Schreek S, Pfeifer S, Reuner F, Vollger L, Stanulla M, von Kockritz-Blickwede M, Glander S, Barczyk-Kahlert K, von Kaisenberg CS, Friesenhagen J, Fischer-Riepe L, Zenker S, Schultze JL, Roth J, Viemann D. S100-alarmin-induced innate immune programming protects newborn infants from sepsis. Nat Immunol 2017;18: 622-632. Abstract |
2013
Grabner A, Kentrup D, Edemir B, Sirin Y, Pavenstadt H, Schlatter E, Schober O, Schafers M, Schnockel U, Reuter S. PET with 18F-FDG-labeled T lymphocytes for diagnosis of acute rat renal allograft rejection. J Nucl Med 2013;54: 1147-1153. Abstract |