Trachea of a fruit fly embryo (Drosophila). The cell membranes were labelled with different fluorescent proteins using the “brainbow” technique. The membranes of some cells (left) were segmented and displayed using software.
© Dominique Förster, Stefan Luschnig

International CiM Symposium 2017

Cells in Motion: Pattern formation in space and time
Münster, 4–6 September 2017

Meeting venue: lecture hall GEO1, Heisenbergstraße 2

Photos

Monday evening at the CiM Symposium – a chance to gain new insights and talk about research.
© CiM - Roberto Schirdewahn
  • Positive atmosphere in the lecture hall: The audience got to hear a variety of exciting talks from the international speakers.
    © CiM - Roberto Schirdewahn
  • The Symposium attracted a large number of interested listeners over the entire three days.
    © CiM - Roberto Schirdewahn
  • Benny Shilo from the Weizmann Institute of Science showed how there is a blueprint for all life.
    © CiM - Roberto Schirdewahn
  • Junior researchers from the Cluster of Excellence were also given a platform …
    © CiM - Roberto Schirdewahn
  • … to present their research to the audience.
    © CiM - Roberto Schirdewahn
  • From the lecture hall to the posters: in the evenings, the researchers got talking to each other in a relaxed atmosphere …
    © CiM - Roberto Schirdewahn
  • … and showed a lot of interesting examples of the research being undertaken in CiM groups.
    © CiM - Roberto Schirdewahn

When an organism develops, cells arrange themselves into three-dimensional structures and form patterns. Within cells too, the individual cell components produce patterns which then lead to certain functions. But what are the processes behind this? This was the question that international researchers discussed at the University of Münster in September 2017. At the symposium being hosted by the Cells-in-Motion (CiM) Cluster of Excellence, speakers from the fields of chemistry, mathematics, physics, biology and medicine presented their current research results.

The topics they talked about ranged from molecular self-organisation and membrane organisation as well as cell-matrix and cell-cell interactions to tissue morphogenesis and mechanobiology. Another focus of the symposium was on various imaging technologies which researchers use to visualize the patterns and the processes being investigated. The symposium provided a platform not only for the speakers who had been invited from all over the world and for the CiM group leaders, but also for junior researchers from the Cluster of Excellence to present their research.

  • Conference Programme

    International CiM Symposium 2017 | Cells in Motion: Pattern formation in space and time | 4-6 September 2017
    Meeting venue: lecture hall GEO1, Heisenbergstraße 2

    Free Childcare Available: Your children will be looked after during the afternoon session on site or at your home by the Zauberfrau. Toys will also be available. To make use of this offer please contact the CiM Office until Friday 2:00 p.m via e-mail.

    Monday, 4 September 2017

    11:00 Registration
    13:00 Welcome

    Session 1: Membrane Organisation

    (Chair: Volker Gerke)

    13:10 Matthias Röger, TU Dortmund University
    Interaction of membrane localized reactions and transport-diffusion processes in cells
    13:40 Milos Galic, University of Münster
    Nanoscale membrane deformations control lamellipodia re-initiation, cell shape and migration pattern
    14:00 Patricia Bassereau, Institute Curie, Paris
    Coupling curved membranes to cytoskeleton: I-BAR  proteins and Ezrin
    14:30 Coffee break

    Session 2: Visualising and Manipulating Processes in Living Cells

    (Chair: Andrea Rentmeister)

    15:00 Nathan Luedtke, University of Zurich
    Harnessing bioorthogonal chemistry to visualize embryogenesis and drug metabolism
    15:30 Benjamin Schumann, Stanford University
    Glycosyltransferase bump-hole engineering to dissect mucin-type O-glycosylation in living cells
    15:45 Markus Affolter, University of Basel
    Angiogenesis live: new insights and new tools
    16:15 Coffee break

    Session 3: Young Investigator Talks

    (Chairs: Milos Galic & Sebastian Rumpf)

    16:45 Johannes Fels, University of Münster
    Acute hydrostatic pressure activates apical acto-myosin in endothelial cells
    17:00 Robert Meissner, University of Münster
    In vivo shear stress development and blood flow manipulation
    17:15 Maniraij Bhagawati, University of Münster
    Traceless fluorescent labeling of cell-surface receptors using a novel split intein
    17:30 Kathleen Hübner, University of Münster
    Wnt signaling regulates vascular pattern formation and anastomosis in the CNS
    17:45 Sargon Yigit, University of Münster
    Characterization of cell-environment interactions affecting single-cell migration in vivo
    18:00 short break to change session

    Evening Programme

    (Chair: Erez Raz)

    18:10 Benny Shilo, Weizmann Institute of Science
    Life's blueprint
    from 19:00 Reception & poster presentations

    Tuesday, 5 September 2017

    8:30

    Registration

    Session 4: Cell-Cell Interaction

    (Chair: Ryan Gilmour)

    9:00 Arndt Siekmann, University of Münster
    Endothelial Notch signaling limits angiogenesis via control of artery formation
    9:20 Peter A. Markowich, University of Cambridge
    First principle modeling of biological transportation networks
    9:50 Nick A. Meanwell, Bristol-Myers Squibb
    Modulators of hepatitis C virus NS5A
    10:20 Coffee break

    Session 5: Mechanobiology

    (Chair: Timo Betz)

    10:50 Michel Labouesse, Institut de Biologie Paris-Seine
    Tissue viscoplasticity in morphogenesis
    11:20 Maja Matis, University of Münster
    Control of the tissue mechanics via the atypical cadherins Fat and Dachsous
    11:40 Matthieu Piel, Institute Curie, Paris
    Squeezing the nucleus
    12:10 Lunch break

    Session 6: Cellular Communication

    (Chair: Stefan Luschnig)

    13:40 Stephan Sigrist, Freie Universität Berlin
    Pre-post-synaptic communication patterns synaptic terminals in Drosophila
    14:10 Jon Clardy, Harvard Medical School, Boston
    Cellular communications in a two-kingdom symbiosis
    14:40 Kerstin Bartscherer, University of Münster
    Go ahead, grow a head! Mechanisms of regeneration initiation
    15:00 Jan-Frederik Pietschmann, University of Münster
    Phase separation in a model with non-local interactions
    15:20 Coffee break

    Session 7: Frontiers in Imaging

    (Chair: Michael Schäfers)

    15:50 David O'Hagan, University of St Andrews
    Forming 18F-C bonds with an enzyme, for PET
    16:20 Wolfram Pernice, University of Münster
    Photonic nanostructures for imaging applications
    16:40 Erik Sahai, The Francis Crick Institute, London
    Cancer cell invasion in complex environments

    Evening Programme

    19:00 Speakers' Dinner

    Wednesday, 6 September 2017

    8:30

    Registration

    Session 8: Cell Matrix Interaction

    (Chair: Roland Wedlich-Söldner)

    9:00 Zena Werb, University of California, San Francisco
    Intravital imaging reveals cell-matrix interactions during breast development and cancer progression
    9:30 Peter Seeberger, Max Planck Institute of Colloids and Interfaces, Potsdam
    Automated glycan assembly as basis for chemical glycobiology and vaccine development
    10:00 Britta Trappmann, University of Münster
    Regulation of cell fate by extracellular matrix stiffness
    10:20 Martin A. Schwartz, Yale Cardiovascular Research Center, New Haven
    Cell responses to forces applied through integrins
    10:50 Coffee break

    Session 9: Tissue Morphogenesis

    (Chair: Stefan Schulte-Merker)

    11:20 Katie Bentley, Uppsala University, Sweden
    The temporal basis of angiogenesis
    11:50 Sebastian Rumpf, University of Münster
    Spatiotemporal control of dendrite pruning in Drosophila
    12:10 Julien Vermot, University of Strasbourg
    Endocardial morphogenesis: from mechanical forces to cell behaviors

    Closing Remarks

    12:40 Closing remarks
    12:45 Snacks

  • Travel and Hotel Information

    Meeting Venue

    GEO I (Institute of Geography)
    Heisenbergstraße 2, D-48149 Münster

    Directions by plane

    From Münster-Osnabrück Airport (FMO)

    • Via public transport (about 35 minutes): Take bus line S50, R51 or D50. Alight at Münster Central Station (Münster Hauptbahnhof). Please follow the directions by train and bus.

    From Dortmund Airport (DTM), Düsseldorf Airport (DUS), Cologne Bonn Airport (CGN), Frankfurt Airport (FRA)

    • Via public transport (about 1.5-2 hours from DTM/DUS/CGN, about 3-3.5 hours from FRA): Take a train (ICE/IC/RE) to Münster Central Station (Münster Hauptbahnhof). Please follow the directions by train and bus.

    Directions by train

    ICE, IC and EC trains arrive at Münster Central Station (Münster Hauptbahnhof).

    Directions by bus

    All registered symposium participants will receive a ‘Welcome! Münster Ticket’ for free public transport in Münster for the time of the conference. The tickets will be handed out on site at the registration for the symposium.

    Bus options A (from Münster Central Station to Mendelstraße):

    • City bus 2 (gate C1 in front of the train station), direction Alte Sternwarte
    • City bus 13 (gate B2 in front of the train station), direction Technologiepark

    You find the meeting venue right opposite of the bus stop Mendelstraße.

    Bus options B (from Münster Central Station to Schreiberstraße):

    • City bus 5 (gate B2 in front of the train station), direction Hannaschweg
    • City bus 11 (gate C1 in front of the train station), direction Dieckmannstraße

    From the bus stop please turn right into Schreiberstraße and proceed to Heisenbergstraße. The meeting venue is about 8 min walking distance (directions with Google Maps).

    Directions by car

    Address for your navigation system: Domagkstraße 61, D-48149 Münster

    Please use the free parking garage at P+R Coesfelder Kreuz that is located about 8 minutes walking distance from the meeting venue (directions with Google Maps). There are no parking slots available at the meeting venue.

    Hotel

    You can book your hotel in Münster here.

  • Conference Report

    International CiM Symposium | Cells in Motion: Pattern formation in space and time | Münster, 4–6 September 2017

    In a true testament to interdisciplinary research, the 2017 International CiM Symposium was recently held at the WWU Münster and brought together experts from the fields of biomedicine, biology, chemistry, biophysics, mathematics and computer science. The organising committee (T. Betz, R. Gilmour, G. Lenz, S. Luschnig, A. Rentmeister, S. Schulze-Merker, R. Wedlich-Söldner, B. Wirth - all WWU Münster) decided on the alluring conference theme “Patterns in space and time” and left the interpretation entirely up to a veritable who’s who of leading experts. The challenge for each speaker was to effectively convey sophisticated, cutting edge science to a mixed audience of clinicians and natural scientists. The end result was a 3-day tour-de-force in manipulating, studying and understanding cell behaviour.

    Following a general introduction to the Cluster of Excellence “Cells in Motion” by Lydia Sorokin (WWU Münster), the opening session (Chair: Volker Gerker, WWU Münster) on membrane organisation began with a thoughtful discussion of membrane-localised interactions and transport diffusion processes in cells by Mathias Röger (TU Dortmund). From mathematics, the programme then switched to biophysics and a lecture by Milos Galic (WWU Münster) on nanoscale membrane deformations and their effects on lamellipodia re-initiation, cell shape and migration pattern. The session concluded with a lecture from Patricia Bassereau (Institut Cuire, Paris) on I-BAR proteins and Ezrin in the context of curved membranes.

    Session two entitled “Visualising cells and manipulating processes in living cells” (Chair: Andrea Rentmeister, WWU Münster) focussed on the important role of chemistry. The importance of bio-orthogonal reactions in visualising drug metabolism and embryogenesis was emphasised by Nathan Leudtke (University of Zürich). In a switch to glycochemistry, Benjamin Schumann from Stanford University spoke about glycosyltransferase bump-hole engineering to dissect mucin-type O-glycosylation in living cells. The session was rounded off by Markus Affolter (University of Basel) who delivered a lecture entitled “Angiogenesis live: new insights and new tools”.

    Session three, chaired by Milos Galic and Sebastian Rumpf, provided a platform for young investigators funded through the CiM Cluster to share their latest results.  Speakers included Johannes Fels, Robert Meissner, Maniraij Bhagawati, Kathleen Hübner and Sargon Yigit (all WWU Münster).

    The evening lecture, chaired by Erez Raz (WWU Münster), was delivered by Benny Shilo from the Weizmann Institute of Science. Entitled “Life’s Blueprint”, this talk was a beautiful balance between scientific complexity and everyday occurrences. Peppered with photographs and images from Shilo’s own collection, the lecture was both accessible and expressive. The first day of the meeting concluded with a reception and poster presentations.

    The first session on Tuesday 5th September (Session 4. Chair: Ryan Gilmour, WWU Münster) kicked off with Arndt Siekmann’s (WWU Münster) lecture entitled “Endothelial notch signalling limits angiogenesis via controlled artery formation”. This was followed by a change in pace to mathematics and a presentation on modelling biological transport mechanisms by Peter Markowich from the University of Cambridge. The session concluded with the sole industrial speaker, Nick Meanwell (Bristol-Myers Squibb) who gave an account of BMS’s development of modulators of hepatitis C virus NS5A.

    Session 5 focussed on the theme of mechanobiology (Chair: Timo Betz, WWU Münster), beginning with the subject of tissue viscoplasticity in a lecture by Michel Labouesse (Institut de Biologie Paris-Seine). Maja Matis (WWU Münster) addressed the control of tissue mechanics, before handing the floor to Matthieu Piel (Institut Curie, Paris) for lecture intriguingly entitled “Squeezing the nucleus”.

    Central to the CiM initiative is cellular communication, the subject of session 6 chaired by Stefan Lusching. Commencing with a lecture by Stefan Sigrist (FU Berlin) on pre-post-synaptic communication patterns, the session then moved to chemical ecology and explored cellular communications in a two-kingdom symbiosis (Jon Clardy, Harvard University). Regeneration was the subject of the next lecture by Kerstin Bartscherer (MPI for Molecular Biomedicine), specifically understanding the mechanism of this process in flatworms. The final talk of this session moved to mathematics, and was given by Jan-Frederik Pietschmann (WWU Münster) on the development of a phase separation model with non-local interactions.

    Following a short break, the second day concluded with session 6 entitled “Frontiers in imaging” chaired by Michael Schäfers (WWU Münster). David O’Hagan (St Andrews) demonstrated how an enzyme can be harnessed to form 18F-C bonds for application in positron emission tomography. Wolfram Pernice (WWU Münster) shared his lab’s latest results in the field of photonic nanostructures for imaging, and the session concluded with a lecture from Erik Sahai (The Francis Crick Institute, London) on cancer cell invasion in complex environments. The programme concluded with a Speakers Dinner held in the Picasso Museum, Münster (Restaurant La Californie) and a convivial evening was had by all.

    The final day (Session 8, Chair: Roland Wedlich-Söldner, WWU Münster) was opened by Zena Werb from the University of California, San Francisco who discussed cancer progression and the importance of intravital imaging in revealing cell-matrix interactions. Peter Seeberger (MPI Potsdam) then discussed the importance of chemical glycobiology as a platform for vaccine development, and demonstrated how automated glycan assembly can facilitate this process. The penultimate lecture of the session was delivered by Britta Trappmann from the bioactive materials lab at the MPI in Münster entitled “Regulation of cell fate by extracellular matrix stiffness”. The finale to session 8 was provided by Martin Schwartz (Yale Cardiovascular Research Group) who lectured on the response of cells to forces applied through integrins.

    Session 9 was devoted to tissue morphogenesis and chaired by Stefan Schulte-Merker (WWU Münster). The temporal basis of angiogenesis was addressed by Katie Bentley (Uppsala University) before a switch in themes to spatiotemporal control of dendrite pruning in Drosophila by Sebastian Rumpf (WWU Münster).

    The 2017 International CiM Symposium was a huge success thanks to the 31 national and international speakers who were so very generous in sharing both their time and expertise. On behalf of the organising committee, we would like to express our deepest gratitude. Of course, a meeting of this complexity would not have been possible without the   organisational support of our very dedicated CiM Office team, and in particular Ann-Christin Dietrich and Christel Marx

    The symposium was an excellent way to celebrate the Cluster’s success in Münster, but perhaps more importantly it provided inspiration and motivation for the future. We look forward to welcoming you to the next meeting in 2019!

    Christiane Natsch and Ryan Gilmour

Organising Committee