Quantitative assessment of endothelial permeability by novel scalable BODIPY nanostructures

Principal investigators: Gustavo Fernandez Huertas, Max Masthoff
Project number: CRC 1450 B01
Project term: 01/2021–12/2024

Graphical abstract
© CRC inSight

In this project, we aim at a novel multimodal imaging approach using BODIPY-based nanostructures to investigate endothelial permeability upon inflammatory stimuli in vivo. The tunable size (ranging from few nanometers to several micrometers) and shape (spherical, 1D, 2D) of the nanostructures will allow for a quantitative assessment of endothelial barrier function using the entire range of multiscale imaging modalities applied in this CRC (1, 2).

We will evaluate our BODIPY-based approach in inflammatory pathologies associated with changes in endothelial permeability such as ischemia-reperfusion (I/R; heart, kidney) and sepsis (3).


Principal investigators

Project members


The names of the principal investigators in our network have been bolded. Publications released prior to 2021, when funding for our network commenced, represent previous project-related work.


Masthoff M, Freppon FN, Zondler L, Wilken E, Wachsmuth L, Niemann S, Schwarz C, Fredrich I, Havlas A, Block H, Gerwing M, Helfen A, Heindel W, Zarbock A, Wildgruber M, Faber C. Resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking. EBioMedicine 2021;73: 103670. Abstract


Helmers I, Shen B, Kartha KK, Albuquerque RQ, Lee M, Fernandez G. Impact of Positional Isomerism on Pathway Complexity in Aqueous Media. Angew Chem Int Ed Engl 2020;59: 5675-5682. Abstract


Masthoff M, Buchholz R, Beuker A, Wachsmuth L, Kraupner A, Albers F, Freppon F, Helfen A, Gerwing M, Holtke C, Hansen U, Rehkamper J, Vielhaber T, Heindel W, Eisenblatter M, Karst U, Wildgruber M, Faber C. Introducing Specificity to Iron Oxide Nanoparticle Imaging by Combining (57)Fe-Based MRI and Mass Spectrometry. Nano Lett 2019;19: 7908-7917. Abstract


Masthoff M, Gran S, Zhang X, Wachsmuth L, Bietenbeck M, Helfen A, Heindel W, Sorokin L, Roth J, Eisenblatter M, Wildgruber M, Faber C. Temporal window for detection of inflammatory disease using dynamic cell tracking with time-lapse MRI. Sci Rep 2018;8: 9563. Abstract
Masthoff M, Helfen A, Claussen J, Karlas A, Markwardt NA, Ntziachristos V, Eisenblatter M, Wildgruber M. Use of Multispectral Optoacoustic Tomography to Diagnose Vascular Malformations. JAMA Dermatol 2018;154: 1457-1462. Abstract
Sampedro A, Ramos-Torres A, Schwoppe C, Muck-Lichtenfeld C, Helmers I, Bort A, Diaz-Laviada I, Fernandez G. Hierarchical Self-Assembly of BODIPY Dyes as a Tool to Improve the Antitumor Activity of Capsaicin in Prostate Cancer. Angew Chem Int Ed Engl 2018;57: 17235-17239. Abstract


Rödle A, Lambov M, Mück-Lichtenfeld C, Stepanenko V, Fernández G. Cooperative nanoparticle H-type self-assembly of a bolaamphiphilic BODIPY derivative in aqueous medium. Polymer 2017;128: 317-324. Abstract


Rodle A, Ritschel B, Muck-Lichtenfeld C, Stepanenko V, Fernandez G. Influence of Ester versus Amide Linkers on the Supramolecular Polymerization Mechanisms of Planar BODIPY Dyes. Chemistry 2016;22: 15772-15777. Abstract


Reifschneider O, Wentker KS, Strobel K, Schmidt R, Masthoff M, Sperling M, Faber C, Karst U. Elemental bioimaging of thulium in mouse tissues by laser ablation-ICPMS as a complementary method to heteronuclear proton magnetic resonance imaging for cell tracking experiments. Anal Chem 2015;87: 4225-4230. Abstract


Allampally NK, Florian A, Mayoral MJ, Rest C, Stepanenko V, Fernandez G. H-aggregates of oligophenyleneethynylene (OPE)-BODIPY systems in water: guest size-dependent encapsulation mechanism and co-aggregate morphology. Chemistry 2014;20: 10669-10678. Abstract
Schmidt R, Nippe N, Strobel K, Masthoff M, Reifschneider O, Castelli DD, Holtke C, Aime S, Karst U, Sunderkotter C, Bremer C, Faber C. Highly Shifted Proton MR Imaging: Cell Tracking by Using Direct Detection of Paramagnetic Compounds. Radiology 2014;272: 785-795. Abstract