Improving intravital microscopy of inflammatory cell response by active motion compensation with controlled adaptive optics

Principal investigators: Thomas Huser, Benedikt Wirth
Project number: CRC 1450 A06
Project term: 01/2021–12/2024

Graphical abstract
© CRC inSight

We will advance multiphoton fluorescence microscopy by developing a novel optical module comprised of a high-speed deformable mirror that will actively compensate tissue motion during intravital imaging (1, 2), for instance due to heart beat (8 Hz), breathing (3 Hz, in mm-range) or peristaltic movement of the gut in mice. To control this module in real-time, we will develop and validate mathematical methods (3, 4) that track and predict tissue deformation.

This will allow imaging of inflammatory processes at cellular resolution without mechanical tissue fixation (5).


Principal investigators

Project members


The names of the principal investigators in our network have been bolded. Publications released prior to 2021, when funding for our network commenced, represent previous project-related work.


Guastini M, Rajković M, Rumpf M, Wirth B. The Variational Approach to the Flow of Sobolev-Diffeomorphisms Model. Scale Space and Variational Methods in Computer Vision 2023: 551-564.
Kong C, Pilger C, Kunisch M, Förster C, Schulte am Esch J, Huser T. Hyperspectral Coherent Raman Scattering (CRS) Microscopy Based on a Rapidly Tunable and Environmentally Stable Fiber Laser. Laser & Photonics Reviews 2023;17Abstract


Striewski P, Wirth B. Elastic 3D–2D Image Registration. J Math Imaging Vis 2022;64: 443-462. Abstract


Effland A, Heeren B, Rumpf M, Wirth B. Consistent curvature approximation on Riemannian shape spaces 2021;42: 78-106. Abstract
Hardering H, Wirth B. Quartic Lp-convergence of cubic Riemannian splines. IMA Journal of Numerical Analysis 2021Abstract
Kong C, Bobe S, Pilger C, Lachetta M, Oie CI, Kirschnick N, Monkemoller V, Hubner W, Forster C, Schuttpelz M, Kiefer F, Huser T, Schulte Am Esch J. Multiscale and Multimodal Optical Imaging of the Ultrastructure of Human Liver Biopsies. Front Physiol 2021;12: 637136. Abstract


Heeren B, Rumpf M, Wirth B. Variational time discretization of Riemannian splines. IMA Journal of Numerical Analysis 2019;39: 61–104. Abstract
Markwirth A, Lachetta M, Monkemoller V, Heintzmann R, Hubner W, Huser T, Muller M. Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction. Nat Commun 2019;10: 4315. Abstract


Berkels B, Wirth B. Joint denoising and distortion correction of atomic scale scanning transmission electron microscopy images. Inverse Problems 2017;33: 095002. Abstract
Song J, Zhang X, Buscher K, Wang Y, Wang H, Di Russo J, Li L, Lutke-Enking S, Zarbock A, Stadtmann A, Striewski P, Wirth B, Kuzmanov I, Wiendl H, Schulte D, Vestweber D, Sorokin L. Endothelial Basement Membrane Laminin 511 Contributes to Endothelial Junctional Tightness and Thereby Inhibits Leukocyte Transmigration. Cell Rep 2017;18: 1256-1269. Abstract


Absil P-A, Gousenbourger P-Y, Striewski P, Wirth B. Differentiable Piecewise-Bézier Surfaces on Riemannian Manifolds. SIAM J. Imaging Sci. 2016;9: 1788-1828. Abstract
Diekmann R, Wolfson DL, Spahn C, Heilemann M, Schuttpelz M, Huser T. Nanoscopy of bacterial cells immobilized by holographic optical tweezers. Nat Commun 2016;7: 13711. Abstract
Muller M, Monkemoller V, Hennig S, Hubner W, Huser T. Open-source image reconstruction of super-resolution structured illumination microscopy data in ImageJ. Nat Commun 2016;7: 10980. Abstract


Dale BM, McNerney GP, Thompson DL, Hubner W, de Los Reyes K, Chuang FYS, Huser T, Chen BK. Cell-to-cell transfer of HIV-1 via virological synapses leads to endosomal virion maturation that activates viral membrane fusion. Cell Host Microbe 2011;10: 551-562. Abstract
Rumpf M, Wirth B. An Elasticity-Based Covariance Analysis of Shapes. Int J Comput Vis 2011;92: 281-295. Abstract


Hubner W, McNerney GP, Chen P, Dale BM, Gordon RE, Chuang FYS, Li X-D, Asmuth DM, Huser T, Chen BK. Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. Science 2009;323: 1743-1747. Abstract