Improving intravital microscopy of inflammatory cell response by active motion compensation using controlled adaptive optics

Principal investigators: Thomas Huser, Benedikt Wirth
Project number: CRC 1450 A06
Project term: 01/2021–12/2028

Our developed methods can already compensate periodic motions of vessels (top: uncompensated, bottom: compensated); to identify additional nonpredictable motion components we will complement the imaging system with fast 3D optical coherence tomography.
© CRC inSight A06

We will advance multiphoton fluorescence microscopy by developing a compact optical motion compensation module that will actively compensate tissue motion during intravital imaging, for instance due to heart beat (8 Hz), breathing (3 Hz, in mm-range) or peristaltic movement of the gut in mice. The module will be comprised of a high-speed deformable mirror and optics to facilitate extended focus optical coherence tomography (xfOCT). Three-dimensional xfOCT data will be processed in real time and, in combination with novel mathematical methods that track and predict tissue deformation, these will be used to track and image inflammatory processes with cellular resolution.

Team

Principal investigators

Project members

Publications

The names of the principal investigators in our network have been bolded. Publications released prior to 2021, when funding for our network commenced, represent previous project-related work.