Research Projects
At the University of Münster, many different projects from diverse research areas use mass spectrometry. Here, some of them are briefly introduced.
| Research Project | Description | Instruments |
|---|---|---|
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Occurrence and bioavailability of polycyclic aromatic compounds (PAC) Prof. Dr. C. Achten |
In the group of PAC, including polycyclic aromatic hydrocarbons (PAH) and NSO-PAC, thousands of substances are summarized. The 16 EPA-PAH only represent a very small part of this group. Toxic non-EPA-PAH need to be identified and included in regulation by (a) development of appropriate analytical procedures, (b) studying their occurrence in the environment and (c) toxicity and bioavailability. | GC-APLI-TOFMS |
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Aminosteroids and Aminotriterpenes as antiprozoal agents Prof. Dr. T. Schmidt |
We identified Aminosteroids and Aminotriterpenes from Apocyncaceae and Buxaceae as very potent antiprotozoal agents, especially against Malaria and Sleeping Sickness. Search for further compounds of these types in related plants is under way in various projects. | UHPLC-QTOF (Bruker MicrOTOF QII with Dionex RSLC 3000) |
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ADMET properties of Sesquiterpene lactones as antiprotozoal agentes Prof. Dr. T. Schmidt |
Sesquiterpene lactones from Arnica are currently under study as new medication for cutaneous Leishmaniasis. Here we investigate the biopharmaceutical behaviour (skin penetration, metabolism, pharmacokinetics) of these promising natural products. | UHPLC-QTOF (Bruker MicrOTOF QII with Dionex RSLC 3000) |
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Essential oils as anti-Mycetoma agents Prof. Dr. T. Schmidt |
Certain essential oils were discovered by us to have strong activity against the pathogens of Mycetoma, a fungal/bacterial neglected disease. We strive to find the constituents responsible for this activity. | GC-QTOF MS (Agilent 7250 GC/QTOF mit 7890 GC) |
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Studies on the structure, function and interaction of non-ribosomal peptide synthetases Prof. Dr. H. Mootz |
The modules of non-ribosomal peptide synthetases (NRPS) involved in the biosynthesis of numerous antibiotic active ingredients are a research subject that we are working on using bioanalytical mass spectrometry. Using high-resolution, LC-coupled MS on intact proteins, we monitor the loading of individual NRPS modules with amino acids and quantify the di- and tripeptides formed by the interaction of several modules. Using nano-LC-MS² studies on proteolytic digests of NRPS modules with specifically incorporated photoreactive amino acids, we investigate both the interaction between modules and structural changes during the catalytic cycle of peptide formation. |
nanoLC- / UHPLC-HR-QTOF (Bruker maXis II mit Thermo UltiMate™ 3000 RSLC und RSLCnano) |
|
Instrumentation, method development and applications of biomolecular MS Prof. Dr. S. König |
Focus on protein and peptide analysis with RP-LC-MS/MS, but also investigation of small molecules. |
Synapt G2 Si Q-TOF Premier Esquire 3000 |
|
Proteomics of gastritis, gastric ulcer and cancer Prof. Dr. S. König |
A diagnosis of gastric cancer relies on histopathology. Endoscopy rates are increasing. Helicobacter pylori infection is a major risk factor. To elucidate abundant blood biomarkers, and reduce the number of diagnostic surgical interventions, we investigated sera and biopsies from a cohort of 219 H. pylori positive and negative patients diagnosed with gastric cancer, gastritis, and ulcers. Cooperation project with the University of Islamabad. |
Synapt G2 Si |
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Serum activity of angiotensin and carboxypeptidase N/renin-angiotensin system Prof. Dr. S. König |
Development of a neuropeptide reporter assay for research into Sudeck's disease and other diseases, complementary serum protein expression measurement. | Synapt G2 Si |
|
Determination of protoporphyrin IX in the serum of glioblastoma patients Prof. Dr. S. König |
Determination and characterisation of protoporphyrin IX biomarkers in the serum of glioblastoma patients. Cooperation with the neurosurgery of the UKM. |
Esquire 3000 |
|
Analysis of lipids (e.g. fatty acids, phospholipids, sphingolipids, Lipid A, lipopeptides) Prof. Dr. H. Hayen |
Identification and quantification of free and esterified fatty acids: Hydrolysis and derivatization of fatty acids prior GC-MS analysis |
GC-MS (EI or CI) |
|
Determination of polar metabolites (e.g. trimethylamine, coenzyme A derivatives, oxalic acid) Prof. Dr. H. Hayen |
Quantification of selected metabolites, e.g. oxalic acid, by LC-MS/MS |
Online SPE-ESI-MS (Q Exactive plus or TSQ Vantage) |
|
Characterization of biosurfactants (e.g. rhamnolipids, sophorolipids) Prof. Dr. H. Hayen |
Identification and quantification of rhamnolipids by HPLC or SFC coupled to HR-MS/MS or triple quadrupole MS/MS |
RP-HPLC-ESI-MS and SFC-ESI-MS (Q Exactive plus or TSQ Vantage) |
|
MEET |
Non-aqueous electrolytes and ionic liquids are important components in lithium ion batteries. Up to now, numerous investigations on the characterization of degradation products, including structural, qualitative and quantitative information were performed as well as the proposal of reaction mechanisms. But still, the degradation products and mechanisms are not explained in detail or verified in literature. | SPME/HS-GC-MS, GC-OrbiTrap, 2D-IC-MS, LC-IT-TOF-MS, CE-QTOF-MS, GC-APCI-QTOF-MS, CE-MS, Hyphenation to ICP-MS |
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Interphases, Transition Metal Dissolution, Lithium Distribution MEET |
Metal dissolution and metal migration of the cathode and the corresponding deposition of these metals on the graphitic anode are known harmful degradation effects, especially for the formed solid electrolyte interphase on the surface of the anode. In addition, lithium can be “lost” or immobilized during charge/discharge and is therefore no longer available as an electrochemically active cation. | LA-ICP-MS, GD-MS, ToF-SIMS |
|
MEET |
LIB material characterization is inevitable in the context of material recycling. Evaluation and adjustment of recycling procedures requires reliable and comprehensive information of the feedstock, which means reverse engineering in most cases since no information about e.g. cell chemistry is available. | SPME/HS-GC-MS, GC-OrbiTrap, 2D-IC-MS, LC-IT-TOF-MS, CE-QTOF-MS, GC-APCI-QTOF-MS, CE-MS, Hyphenation to ICP-MS, LA-ICP-MS, GD-MS, ToF-SIMS |
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The nature and abundance of Earth’s ‘depleted’ mantle Prof. Dr. A. Stracke |
We use radiogenic isotope ratios of Sr-Nd-Hf-Pb measured by TIMS and MC-ICPMS, as well as trace element concentrations measured by SF-ICPMS and LA-ICPMS in oceanic mantle rocks, abyssal peridotites, basalts and melt inclusions to investigate the chemical evolution of Earth’s mantle. | MC-ICPMS, TIMS, SF-ICPMS, LA-ICPMS |
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Human Biomonitoring of Mycotoxins Prof. Dr. H. Humpf |
Assessment of the exposure to mycotoxins, the toxic metabolites of mold, is a critical step for risk assessment. Based on UHPLC-MS/MS systems with optional online SPE, we apply and further develop state-of-the-art methodologies for assessment mycotoxins and mycotoxin metabolites in blood and urine samples in cohorts up to several thousand participants. | UHPLC-MS/MS (Sciex 7500, QTrap 6500) |
|
Prof. Dr. H. Humpf |
For a comprehensive understanding of the mode of action of bioactive substances, it is essential to investigate their influence on the metabolome in the cell. By analyzing a broad spectrum of target analytes, changes, activations and impairments of a broad spectrum of metabolic pathways can be visualized. | UHPLC-MS/MS (Bruker Evoq Elite) UHPLC-QTOF (Bruker Impact II) |
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Impact of food processing on contaminants Prof. Dr. H. Humpf |
Thermal food processing such as roasting, baking, toasting or extrusion cooking has a strong impact on food quality as well as sensory properties. These processes can additionally lead to a reduction of levels of unwanted contaminants such as mycotoxins, improving food safety. However, during thermal processes, also unwanted, process-related contaminants such as acrylamide and furans are formed. Here we investigate the effect of different processes and technologies to improve food quality by reducing the formation of process related contaminants and optimizing the degradation of mycotoxins. | UHPLC-MS/MS (Sciex 5500 QTrap) GC-MS (Agilent 5975C, SPME) |
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Evolution and genetic architecture of chemical communication Prof. Dr. J. Gadau |
We use a combination of detailed phenotypic analysis (GC-MS, quantitative genetics (QTL analysis,qPCR), genetic manipulations to determine and confirm which genes/gene families are involved in chemical communication involved in colony, caste and species recognition. | GC-MS/MS (GC-QQQ) |
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Characterization of protein glycosylation Dr. M. Mormann |
Glycoproteins are key players in the development, growth, function, or survival of an organism and their relationship of structure, location and function is an important feature in life sciences. Thus, we are aiming at the development and implementation of techniques and methodologies to probe protein N- and O-glycosylation. |
Waters SYNAPT G2-S Mass Spectrometer |
|
Prof. Dr. K. Dreisewerd |
MALDI-2 is a new postionization method that can enhance the ion yields, and thus improves the detection limits for numerous classes of analytes. In fundamental studies we aim to better understand and further enhance this and further postionization techniques. |
SYNAPT G2-S and Q Exactive plus orbitrap, both with MALDI-2 modules; the SYNAPT is equipped with a wavelength tunable UV-laser and both UV- and IR-lasers for material ablation |
|
Prof. Dr. K. Dreisewerd |
A major application of the postionization techniques is its use in MS imaging. In this regard we cooperate with numerous expert colleagues and within larger consortia to apply the method. Just two out many examples would be the visualization of retina and infected tissue | TimsTOF fleX MALDI-2 and Q Exactive plus orbitrap with MALDI-2 ion source |
|
Prof. Dr. K. Dreisewerd |
To enhance the lateral resolution of the MSI measurement to the µm-level we develop transmission mode MALDI-2 instrumentation. | Q Exactive plus orbitrap |
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Single-cell and correlative analysis Prof. Dr. K. Dreisewerd |
Development of techniques that enable deciphering the chemical heterogeneity of single cells from including MSI, optical, and bioinformatics tools. Concerning the correlative analyses, a special focus is on neutrophils within the SFB/TRR 332 “Neutrophile Granulozyten: Entwicklung, Verhalten und Funktion”. |
timsTOF fleX MALDI-2, Q Exactive plus orbitrap with MALDI-2 ion source |
|
Prof. Dr. K. Dreisewerd |
The application of MALDI-MSI for deciphering the chemical composition of bacterial cultures and biofilms is relatively new but comes with a high potential for microbiological research. We develop methods that can enable a robust and safe analysis of various bacterial strains (including pathogens) and co-cultures competing strains. A special focus is on the use of the method within the SPP 2389 “Emergent Functions of Bacterial Multicellularity” (SPP 2389)”. | TimsTOF fleX MALDI-2, SYNAPT G2-S with MALDI-2 ion source |
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Bioanalysis of drugs from small volumes Prof. Dr. G. Hempel |
Quantitative determination of drugs by VAMS and DBS in biological fluids (capillary blood, venous blood, plasma, etc.) mainly from the therapeutic areas of antipsychotics, antibiotics, cytostatics and antifungals |
UHPLC-MS/MS (Sciex QTrap 6500+) |
|
Prof. Dr. G. Hempel |
Population pharmacokinetics and pharmacokinetics dynamics modeling. |
UHPLC-MS/MS (Sciex QTrap 6500+) |
|
Prof. Dr. M. Lehr |
Enzyme assay studies for inhibitor screening of potential therapeutic substances in the area of cytosolic phospholipase A2a, phospholipase C, fatty acid amid hydrolase, monoacylglycerol lipase and copper containing amine oxidase 3 (AOC3) |
UHPLC-MS/MS (Sciex QTrap 6500+) UHPLC-QTOF (Bruker MicrOTof QII with Dionex RSLC 3000) |
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Prof. Dr. M. Lehr |
Preclinical pharmacokinetics
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UHPLC-MS/MS (Sciex QTrap 6500+) UHPLC-QTOF (Bruker MicrOTof QII with Dionex RSLC 3000) |
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Prof. Dr. M. Lehr |
Lipidomics of
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UHPLC-MS/MS (Sciex QTrap 6500+) UHPLC-QTOF (Bruker MicrOTof QII with Dionex RSLC 3000) |
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Prof. Dr. B. Wünsch |
Preclinical pharmacokinetics
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UHPLC-MS/MS (Sciex QTrap 6500+) |
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Determination of Receptor Affinity Prof. Dr. B. Wünsch |
Determination of receptor affinity of potential therapeutic compounds using non-radiolabeled reference compounds. |
UHPLC-MS/MS (Sciex QTrap 6500+) |
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Reaction Control and Structure Confirmation of Synthetic Compounds Prof. Dr. B. Wünsch |
Reaction controls and structure confirmation of synthetic compounds from drug discovery. | UHPLC-MS/MS (Sciex QTrap 6500+) |
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Bioanalytic of Small Molecules Dr. J. Fabian |
Quantitative determination of small molecules in different biological matrices. |
UHPLC-MS/MS (Sciex QTrap 6500+) UHPLC-QTOF (Bruker MicrOTof QII with Dionex RSLC 3000) |
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Structural Elucidation of Small Molecules Dr. J. Fabian |
Structural elucidation of small molecules
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UHPLC-MS/MS (Sciex QTrap 6500+) UHPLC-QTOF (Bruker MicrOTof QII with Dionex RSLC 3000) |
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Dr. J. Fabian |
Lipidomics
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UHPLC-MS/MS (Sciex QTrap 6500+) UHPLC-QTOF (Bruker MicrOTof QII with Dionex RSLC 3000) |
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CANMORE: N-terminal modifications of plastid-encoded proteins Prof. Dr. I. Finkemeier |
Chloroplasts primarily function as bioreactors converting light into chemical energy. In addition, they also act as regulatory hubs for intracellular communication and mediation of environmental impacts. Chloroplast proteins are encoded either by the plastid or nuclear genome. Several chloroplast multi-protein complexes, are assembled from plastid- as well as nuclear-encoded protein subunits. All nuclear and plastid-encoded chloroplast-localized proteins undergo many co- and post-translational modifications. The aim of CANMORE is to elucidate the role(s) of specific plastid N-terminal modifications (NPMs) and their regulatory interdependency with other PTMs including the N-terminal maturation of the RuBisCO large subunit. | nanoLC-MS/MS (Orbitrap Eclipse 480, Orbitrap Eclipse Tribred) |
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REDOX: The cytosolic redox network in plants Prof. Dr. I. Finkemeier |
Thiol redox regulatory networks are a common feature of all plasmatic cell compartments. These redox networks control many processes such as metabolic functions and signaling. This project will address the competition for electrons, the specificity of the interactions and the kinetics of redox processes in the cytosol of Arabidopsis thaliana. The propagation and transmission of an oxidative stimulus is examined in time-resolved measurements. Redox modifications of thiols are analyzed in this system using high-resolution, quantitative mass spectrometry (MS)-based proteomics. | nanoLC-MS/MS (Orbitrap Eclipse 480, Orbitrap Eclipse Tribred) |
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Prof. Dr. I. Finkemeier |
Mitochondria act as central energy converters in most complex organisms and are deeply embedded into the cellular metabolic network. Mitochondrial metabolism is particularly flexible in plants and can rapidly switch between different programs to integrate changing environmental conditions. In this project we address the hypothesis that cyto-nuclear NAD status links mitochondrial metabolic flexibility to reprogramming of nuclear gene expression. We will modulate mitochondrial enzymes in Arabidopsis to re-route subcellular metabolism and understand the physiological mechanisms behind mitochondria-controlled modulation of cyto-nuclear NAD status by post-translational modification and protein interactions studies. | nanoLC-MS/MS (Orbitrap Eclipse 480, Orbitrap Eclipse Tribred) |