High Resolution Light Microscopy

  • CiM-funded

    Leica TCS SP8

    Location: Institute for Cardiovascular Organogenesis and Regeneration, Mendelstrasse 7
    Group: AG Schulte-Merker
    Contact Person: Dr. Laura Lleras Forero
    Tel.: 0251 9802874, Laura.LlerasForero@ukmuenster.de

    Applications & Info:

    • Life imaging with resonant scanner and environmental control, FRET and FRAP, advance tile scan, 3D projections.
  • © Uni MS - Institute of Neurobiology
    CiM-funded

    Leica TCS SP8-DLS laser scanning confocal microscope with “digital light sheet” module

    Location: Institute of Neurobiology, Badestrasse 9
    Group: AG Luschnig
    Contact Person: Julia Sauerwald
    Tel.: 0251 83-21695, sauerwaj@uni-muenster.de

    Applications & Info:

    • Inverted microscope for high-resolution confocal imaging equipped with a resonant scanner and two HyD detectors for fast and sensitive acquisition.
    • DLS module and Hamamatsu Orca Flash camera for light sheet imaging
    • HyVolution software module for deconvolution postprocessing
    • 405, 488, 552, and 638 nm laser lines
    • 10x/0.40, 40x/1.30 and 63x/1.40 objectives

     

  • Olympus IX70 with TIRF objective and TILL Vision Monochromator

    Location: Center for Molecular Biology of Inflammation (ZMBE), Von-Esmarch-Str. 56
    Contact Person: Pia Brinkert
    Tel.: 0251 83-53030, pia.brinkert@uni-muenster.de

    Applications & Info:

    • Total internal reflection microscopy (TIRFM)
    • TIRF and epifluorescence live-cell imaging (heating/CO2 incubation system connected)
    • Only partially motorized!
    • Runs on Metamorph Software
    • Excitation lines: 488 nm, 568 nm
    • Objectives: 10x/0.30, 20x/0.75, 40x/0.75, 60x/TIRFM/1.45/Oil, 100x/1.35/Oil
  • © Institute of Physiology I

    Olympus FluoView 1000 / laser scanning confocal microscope

    Location: Institute of Physiology I
    Group: AG Pape
    Contact Person: Dr. Peter Blaesse
    Tel.: 0251 83-55416, blaesse@uni-muenster.de

    Applications & Info:

    • optimized for imaging of acute tissue slices
    • combined imaging/electrophysiology (patch clamp) setup
    • suitable for slice optogenetics and uncaging experiments (488 nm, 514 nm, 543 nm)
  • © ZMBE

    Zeiss Axio Imager Z1 with spinning disc confocal microscope

    Location: Institute for cell biology
    Group: AG Raz
    Contact Person: Łukasz Truszkowski
    l_trus01@wwu.de, Tel. lab: 0251 83-58618, Tel. office: 0251 83-52110

    Applications & Info:

    • Fluorescence imaging, Confocal imaging
    • Upright microscope capable of confocal imaging, equipped with water-immersive objectives. The system is equipped with Spinning disc and Hamamatsu camera, there are additional 405nm and 355nm laser for photo bleaching and laser ablation experiments.

  • Zeiss Axio Observer.Z1 spinning disc confocal microscope

    Location: Center for Molecular Biology of Inflammation (ZMBE), Von-Esmarch-Str. 56
    Contact Person: Matteo Rizzato
    Tel.: 0251 83-53030, rizzato@uni-muenster.de

    Applications & Info:

    • Long-term live cell imaging possible (VisiScope Cell Explorer Life Cell Imaging System, Zeiss Z1 Axio Observer Z1 stage, Yokogawa CSU22 spinning disc head, incl. incubation chamber, heating, CO2; Zeiss definite focus)
    • Motorized stage with autofocus (Zeiss definite focus)
    • Runs on Metamorph Software
    • Microscopy in optical bottom 96-well plates possible
    • Excitation lines: 488 nm, 561 nm, 640 nm
    • Objectives: 20x, 40x oil, 60x oil
  • © ZMBE

    Zeiss LSM 710 confocal microscope

    Location: Institute for cell biology
    Group: AG Raz
    Contact Person: Łukasz Truszkowski
    l_trus01@wwu.de, Tel. lab: 0251 83-58618, Tel. office: 0251 83-52110

    Applications & Info:

    • Fluorescence imaging
    • Upright microscope capable of confocal imaging, Equipped with water merged objectives. The system is equipped with 2photon system (Coherent,chameleon, 690nm to 1100nm lasers).
  • CiM co-funded

    Zeiss LSM 800 with Airyscan

    Location: Institute of Medical Biochemistry (ZMBE), Von-Esmarch-Str. 56
    Contact Persons: Sophia Koerdt, Tel.: 0251 83-52123,  s.koerdt@uni-muenster.de
    Christian Hartmann, Tel.: 0251 83-52120,  c_hart11@uni-muenster.de

    Applications & Info:

    • Confocal laser scanning microscope with high resolution and enhanced sensitivity
    • Inverted stand: Axio Observer.Z1
    • Motorized stage with piezo insert
    • Excitation lines: 405, 488, 561 and 640 nm
    • Objective lense: 63X/1.4 Oil DIC
    • 2 GaAsP detectors, 1 Airyscan GaAsP detector, transmitted light detector
    • In Airyscan mode 1,7X better resolution in all three dimensions compared to standard confocal imaging
  • © Institute of Anatomy and Molecular Neurobiology

    VisiFluor High Performance Imaging and Electrophysiology set-up - Zeiss Axio Observer.A1

    Location: Institute of Anatomy and Molecular Neurobiology, Vesaliusweg 2-4 (PAN-Zentrum)
    Contact Person: Dr. Johannes Brockhaus
    Tel.: 0251 83-50203, j.brockhaus@uni-muenster.de

    Applications & Info:

    • imaging set-up, optimized for fast Ca2+-measurements
    • digital sCMOS camera „Hamamatsu ORCA-Flash4.0 V2“ (2 Mpixel)
    • microscope LED-illumination system „lumencor SPECTRA X light engine“
    • high speed polychromatic illumination system „VisiChrome“
    • 2D-VisiFRAP Galvo system inc. VS laser module (405nm) for fluorescence recovery / photobleaching
    • different Chroma ET Filter-Sets
    • objectives: 10x/0,25; 40x/1,2; 63x/1,2
    • patch clamp amplifier „HEKA EPC 10 double“
    • high current isolator „WPI A385“ for field stimulation
  • © Institute of Anatomy and Molecular Neurobiology

    VisiScope cell analyser / fluorescence microscope - Zeiss Axio Imager.Z2

    Location: Institute of Anatomy and Molecular Neurobiology, Vesaliusweg 2-4 (PAN-Zentrum)
    Contact Person: Dr. Christian Neupert
    Tel.: 0251 83-50235, cneupert@uni-muenster.de

    Applications & Info:

    • deep-frozen fluorescence camera „Diagnostic Instruments Spot-Xplorer“ (1,4 Mpixel)
    • high-resolution color camera „Diagnostic Instruments Spot-Flex“ (up to 16 Mpixel)
    • objectives: 5x/0,16; 10x/0,3; 20x/0,8; 40x/0,75; 40x/1,30 oil; 63x/1,40 oil; 100x/1,46 oil
    • joystick and software controlled, motorized microscope stage for 8 slides
    • MetaMorph imaging software (6D-multidimensional presentation)
  • © Institute of Anatomy and Molecular Neurobiology

    VisiScope spinning disc confocal microscope for live cell imaging - Zeiss Axio Observer.Z1

    Location: Institute of Anatomy and Molecular Neurobiology, Vesaliusweg 2-4 (PAN-Zentrum)
    Contact Person: Dr. Christian Neupert
    Tel.: 0251 83-50235, cneupert@uni-muenster.de

    Applications & Info:

    • long-term live cell imaging (VisiScope Cell Explorer Life Cell Imaging System)
    • spinning disc confocal scanning head „Yokogawa CSU X1“
    • VS laser module with 4 exitation lines (405nm, 488nm, 561nm, 640nm)
    • incubation chamber for local CO2 gassing
    • objectives: 10x/0,3; 20x/0,8; 40x/1,40 oil; 63x/1,40 oil