Forces exerted by nanostructures induce membrane deformation in cells.

Participation in the Cluster of Excellence

Junior research group 'Nanoscale forces in cells'

My lab investigates the spatio-temporal dynamics of force detection and force regulation in cells. Coordination of intra- and intercellular forces occurs in a number of cellular systems and is believed to be a crucial element that contributes among others to cell architecture (e.g. neuronal arborization), directionality and speed of cell migration, as well as collective cell behavior. Despite its importance, the molecular mechanisms underlying force-control have largely remained unclear. To investigate these force-control mechanisms, my lab combines classical cell biology with nanofabrication, synthetic biology and computational analysis of microscopic images with high spatial and temporal resolution. As part of the Cells-in-Motion Cluster of Excellence, we aim in the long term to address these questions not only in single cells but also in complex biological systems and organisms.  These studies will allow us to better understand how forces acting on the cell surface contribute to cell shape and cell migration within a tissue or affect tissue differentiation.

  • Research area A.3: Functional membrane domains in neuronal and non-neuronal compartmentalization
  • Project FF-2016-03: Symmetry Breaking in Neurons (Christian Engwer, Milos Galic, project time: 07/2016 - 10/2018)
  • Project FF-2015-02: In vitro and in vivo investigations of lipid-based signaling mechanisms (Kay Grobe, Milos Galic, Project time: 07/2015 - 06/2016)
© CiM - Peter Grewer

“In science you start with an idea and create something that wasn’t there before.“

Dr. Milos Galic is a CiM Junior Group Leader. He nudges the membrane of cells with a mechanical finger. Why? He wants to find out the impact of it on cells. Read the interview

CiM Papers

2019

Begemann I, Saha T, Lamparter L, Rathmann I, Grill D, Golbach L, Rasch C, Keller U, Trappmann B, Matis M, Gerke V, Klingauf J, Galic M. Mechanochemical self-organization determines search pattern in migratory cells. Nat. Phys. 2019: epub. Abstract
Viplav A, Saha T, Huertas J, Selenschik P, Ebrahimkutty MP, Grill D, Lehrich J, Hentschel A, Biasizzo M, Mengoni S, Ahrends R, Gerke V, Cojocaru V, Klingauf J, Galic M. ArhGEF37 assists dynamin 2 during clathrin-mediated endocytosis. J Cell Sci 2019;132: epub. Abstract

2018

Saha T, Galic M. Self-organization across scales: from molecules to organisms. Phil. Trans. R. Soc. B 2018;373Abstract
Schuermann S, Steffes G, Manikowski D, Kastl P, Malkus U, Bandari S, Ohlig S, Ortmann C, Rebollido-Rios R, Otto M, Nusse H, Hoffmann D, Klambt C, Galic M, Klingauf J, Grobe K. Proteolytic processing of palmitoylated Hedgehog peptides specifies the 3-4 intervein region of the Drosophila wing. Elife 2018;7: e33033. Abstract
Singh A, Saha T, Begemann I, Ricker A, Nusse H, Thorn-Seshold O, Klingauf J, Galic M, Matis M. Polarized microtubule dynamics directs cell mechanics and coordinates forces during epithelial morphogenesis. Nat Cell Biol 2018;20: 1126-1133. Abstract

2017

Goudarzi M, Tarbashevich K, Mildner K, Begemann I, Garcia J, Paksa A, Reichman-Fried M, Mahabaleshwar H, Blaser H, Hartwig J, Zeuschner D, Galic M, Bagnat M, Betz T, Raz E. Bleb Expansion in Migrating Cells Depends on Supply of Membrane from Cell Surface Invaginations. Dev Cell 2017;43: 577-587.e5. Abstract
Saha T, Rathmann I, Galic M. A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions. J Vis Exp 2017Abstract

2016

Begemann I, Galic M. Correlative Light Electron Microscopy: Connecting Synaptic Structure and Function. Front Synaptic Neurosci 2016;8: 28. Abstract
Reinhard JR, Kriz A, Galic M, Angliker N, Rajalu M, Vogt KE, Ruegg MA. The calcium sensor Copine-6 regulates spine structural plasticity and learning and memory. Nat Commun 2016;7: 11613. Abstract
Saha T, Rathmann I, Viplav A, Panzade S, Begemann I, Rasch C, Klingauf J, Matis M, Galic M. Automated analysis of filopodial length and spatially resolved protein concentration via adaptive shape tracking. Mol Biol Cell 2016;27: 3616-3626. Abstract

2015

Begemann I, Viplav A, Rasch C, Galic M. Stochastic Micro-Pattern for Automated Correlative Fluorescence - Scanning Electron Microscopy. Sci Rep 2015;5: 17973. Abstract
Galic M, Matis M. Polarized trafficking provides spatial cues for planar cell polarization within a tissue. Bioessays 2015;37: 678-686. Abstract

2014

Galic M, Begemann I, Viplav A, Matis M. Force-control at cellular membranes. Bioarchitecture 2014;4: 164-168. Abstract
Galic M, Tsai F-C, Collins SR, Matis M, Bandara S, Meyer T. Dynamic recruitment of the curvature-sensitive protein ArhGAP44 to nanoscale membrane deformations limits exploratory filopodia initiation in neurons. Elife 2014;3: e03116. Abstract
Jeong S, Galic M. Nanocones to study initial steps of endocytosis. Methods Mol Biol 2014;1174: 275-284. Abstract