Wissenschaftlicher Werdegang

  • Studium der Chemie und Biochemie, Ludwig-Maximilians-Universität München (2002 – 2005)
  • Promotion am Magdalen College und am Department of Plant Sciences der Universität Oxford (2005 – 2009)
  • Junior Research Fellow am New College und am Department of Plant Sciences der Universität Oxford (2008 – 2011)
  • Wissenschaftlicher Mitarbeiter am Institut für Nutzpflanzenwissenschaften und Ressourcenschutz der Universität Bonn (2011 – 2013)
  • Leitung einer unabhängigen Emmy Noether Nachwuchsgruppe am Institut für Nutzpflanzenwissenschaften und Ressourcenschutz der Universität Bonn, und am Institut für Biologie und Biotechnologie der Pflanzen an der WWU Münster (seit 2013)
  • Habilitation und venia legendi in Pflanzenphysiologie & Zellbiologie an der Landwirtschaftlichen Fakultät der Universität Bonn (2017)
  • Professor & Leiter der Arbeitsgruppe Plant Energy Biology am Institut für Biologie und Biotechnologie der Pflanzen an der WWU Münster (seit 2017)

Aktelle Forschungsschwerpunkte in der Arbeitsgruppe

  • Dynamik, Flexibilität und Umprogrammierung des Energiestoffwechsels von Pflanzen bei Umweltveränderungen und Stress
  • Besonderheiten in der Funktion und Struktur von Mitochondrien in Pflanzen
  • Entwicklung optogenetischer und biosensorischer Ansätze zur Steuerung und Echtzeiterfassung von Energiephysiologie in lebenden Zellen und ganzen Organismen

Vier Forschungsarbeiten aus der Arbeitsgruppe

De Col V, Fuchs P, Nietzel T, Elsässer M, Voon CP, Candeo A, Seeliger I, Fricker MD, Grefen C, Møller IM, Bassi A, Lim BL, Zancani M, Meyer AJ, Costa A, Wagner S, Schwarzländer M (2017)
ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology. eLIFE. e26770
https://elifesciences.org/articles/26770

Wagner S, Behera S, De Bortoli S, Logan DC, Fuchs P, Carraretto L, Teardo E, Cendron L, Nietzel T, Füßl M, Doccula FG, Navazio L, Fricker MD, Van Aken O, Finkemeier I, Meyer AJ, Szabò I, Costa A, Schwarzländer M (2015)
The EF-hand Ca2+-binding protein MICU choreographs mitochondrial Ca2+ dynamics in Arabidopsis.
Plant Cell. 27 (11): 3190-3212
http://www.plantcell.org/content/27/11/3190.long

Schwarzländer M, Wagner S, Ermakova YG, Belousov VV, Radi R, Beckman JS, Buettner GR, Demaurex N, Duchen MR, Forman HJ, Fricker MD, Gems D, Halestrap AP, Halliwell B, Jakob U, Johnston IG, Jones NS, Logan DC, Morgan B, Müller FL, Nicholls DG, Remington SJ, Schumacker PT, Winterbourn CC, Sweetlove LJ, Meyer AJ, Dick TP, Murphy MP (2014)
The ‘mitoflash’ probe cpYFP does not respond to superoxide.
Nature. 514 (7523): E12-E14
https://www.nature.com/articles/nature13858

Schwarzländer M, Logan DC, Johnston IG, Jones NS, Meyer AJ, Fricker MD, Sweetlove LJ
Pulsing of membrane potential in individual mitochondria: a stress-induced mechanism to regulate respiratory bioenergetics in Arabidopsis. (2012)
Plant Cell. 24 (3): 1188-1201
http://www.plantcell.org/content/24/3/1188

Vier Übersichtsarbeiten aus der Arbeitsgruppe

Schwarzländer M, Fuchs P (2017)
Plant mitochondrial membranes: adding structure and new functions to respiratory physiology.
Curr Opin Plant Biol. 40: 147-157
http://www.sciencedirect.com/science/article/pii/S1369526617301267

Nietzel T, Hochgräfe F, Mostertz J, Schwarzländer M (2017)
Redox regulation of mitochondrial proteins and proteomes by cysteine thiol switches.
Mitochondrion. 33: 72-83
https://www.sciencedirect.com/science/article/pii/S1567724916301106

Wagner S, De Bortoli S, Schwarzländer M, Szabò I (2016)
Mitochondrial Ca2+ regulation in plants versus animals.
J Exp Bot. 67 (13): 3809-3829
https://academic.oup.com/jxb/article/67/13/3809/1749283

Morgan B, Schwarzländer M (2016)
Fluoreszierende Proteinsensoren für die Redoxregulation in lebenden Zellen.
BIOspektrum. 22 (3): 260-263
https://link.springer.com/article/10.1007/s12268-016-0683-2

Research
All energy that we draw from our food originates from the sun. Plants provide the vital link by acting as energy converters.
Similar to man-made power stations, the energy conversion that occurs in the organelles of plant cells requires tight surveillance and dynamic adjustment to meet demands, maintain efficiency and avoid dysfunction. The frequent and often dramatic changes in the environment of plants, such as the day-night transitions or temperature fluctuations makes tailored control mechanisms particularly important. Nevertheless it is poorly understood how regulation of energy conversion works in plants.
We address this question through our research. We focus on mitochondria, which supply a bulk amount of usable cellular energy by respiration and determine photosynthetic efficiency. To dissect the dynamics of mitochondrial function, physiology and signalling in the living plant we develop the use protein sensors and functional imaging techniques that we integrate into hypothesis-driven genetic, biochemical and cell biological approaches.
Our aim is to understand and engineer subcellular energy control mechanisms determining plant performance, stress tolerance and food security.

Publications

  • Nietzel T, Elsässer M, Ruberti C, Steinbeck MJ, Ugalde JM, Fuchs P, Wagner S, Ostermann L, Moseler A, Lemke P, Fricker MD, Müller-Schüssele SJ, Moerschbacher BM, Costa A, Meyer AJ, Schwarzländer M. . ‘The fluorescent protein sensor roGFP2-Orp1 monitors in vivo H2O2 and thiol redox integration and elucidates intracellular H2O2 dynamics during elicitor-induced oxidative burst in Arabidopsis.’ New Phytologist 221. doi: 10.1111/nph.15550.
  • Scuffi D, Nietzel T, Di Fino LM, Meyer AJ, Lamattina L, Schwarzländer M, Laxalt AM, García-Mata C. . ‘Hydrogen Sulfide Increases Production of NADPH Oxidase-Dependent Hydrogen Peroxide and Phospholipase D-Derived Phosphatidic Acid in Guard Cell Signaling.’ Plant Physiology 3. doi: 10.1104/pp.17.01636.
  • Voon CP, Guan X, Sun Y, Sahu A, Chan MN, Gardeström P, Wagner S, Fuchs P, Nietzel T, Versaw WK, Schwarzländer M, Lim BL. . ‘ATP compartmentation in plastids and cytosol of Arabidopsis thaliana revealed by fluorescent protein sensing.’ Proceedings of the National Academy of Sciences of the United States of America 115. doi: 10.1073/pnas.1711497115.
  • Finkemeier I, Schwarzländer M. . ‘Mitochondrial regulation in the photosynthetic cell: principles and concepts.’ In Annual Plant Reviews: Plant Mitochondria, edited by Logan DC, 185-226. 2nd Ed. Wiley.
  • Hartmann SK, Stockdreher Y, Wandrey G, Tehrani HH, Zambanini T, Meyer AJ, Büchs J, Blank LM, Schwarzländer M, Wierckx N. . ‘Online in vivo monitoring of cytosolic NAD redox dynamics in Ustilago maydis.’ Biochim Biophys Acta 1859. doi: 10.1016/j.bbabio.2018.05.012.
  • Wagner S, Van Aken O, Elsässer M, Schwarzländer M. . ‘Mitochondrial energy signaling and its role in the low-oxygen stress response of plants.’ Plant Physiology 176, No. 2: 1156-1170. doi: 10.1104/pp.17.01387.
  • Behera S, Xu Z, Luoni L, Bonza C, Doccula FG, DeMichelis MI, Morris RJ, Schwarzländer M, Costa A. . ‘Cellular Ca2+ signals generate defined pH signatures in plants.’ Plant Cell 30. doi: 10.1105/tpc.18.00655.
  • Schwarzländer M., Fuchs P. . ‘Plant mitochondrial membranes: adding structure and new functions to respiratory physiology.’ Current Opinion in Plant Biology 40, No. null: 147-157. doi: 10.1016/j.pbi.2017.09.002.
  • De Col V., Fuchs P., Nietzel T., Elsässer M., Voon C., Candeo A., Seeliger I., Fricker M., Grefen C., Møller I., Bassi A., Lim B., Zancani M., Meyer A., Costa A., Wagner S., Schwarzländer M. . ‘ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology.’ eLife 6, No. null. doi: 10.7554/eLife.26770.
  • Xing S., Mehlhorn D., Wallmeroth N., Asseck L., Kar R., Voss A., Denninger P., Schmidt V., Schwarzländer M., Stierhof Y., Grossmann G., Grefen C. . ‘Loss of GET pathway orthologs in Arabidopsis thaliana causes root hair growth defects and affects SNARE abundance.’ Proceedings of the National Academy of Sciences of the United States of America 114, No. 8: E1544-E1553. doi: 10.1073/pnas.1619525114.
  • Attacha S., Solbach D., Bela K., Moseler A., Wagner S., Schwarzländer M., Aller I., Müller S., Meyer A. . ‘Glutathione peroxidase-like enzymes cover five distinct cell compartments and membrane surfaces in Arabidopsis thaliana.’ Plant Cell and Environment 40, No. 8: 1281-1295. doi: 10.1111/pce.12919.
  • Nietzel T., Mostertz J., Hochgräfe F., Schwarzländer M. . ‘Redox regulation of mitochondrial proteins and proteomes by cysteine thiol switches.’ Mitochondrion 33, No. null: 72-83. doi: 10.1016/j.mito.2016.07.010.
  • Zambanini T, Hartmann SK, Schmitz LM, Büttner L, Tehrani HH, Geiser E, Beudels M, Venc D, Wandrey G, Büchs J, Schwarzländer M, Blank LM, Wierckx N. . ‘Promoters from the itaconate cluster of Ustilago maydis are induced by nitrogen depletion.’ Fungal Biology and Biotechnology 4. doi: 10.1186/s40694-017-0040-3.
  • Teardo E., Carraretto L., Wagner S., Formentin E., Behera S., De Bortoli S., Larosa V., Fuchs P., Lo Schiavo F., Raffaello A., Rizzuto R., Costa A., Schwarzländer M., Szabò I. . ‘Physiological characterization of a plant mitochondrial calcium uniporter in vitro and in vivo.’ Plant Physiology 173, No. 2: 1355-1370. doi: 10.1104/pp.16.01359.
  • Senkler J., Senkler M., Eubel H., Hildebrandt T., Lengwenus C., Schertl P., Schwarzländer M., Wagner S., Wittig I., Braun H. . ‘The mitochondrial complexome of Arabidopsis thaliana.’ Plant Journal 89, No. 6: 1079-1092. doi: 10.1111/tpj.13448.
  • Loro G., Wagner S., Doccula F., Behera S., Weinl S., Kudla J., Schwarzländer M., Costa A., Zottini M. . ‘Chloroplast-specific in vivo Ca2+ imaging using yellow Cameleon fluorescent protein sensors reveals organelle-autonomous Ca2+ signatures in the stroma.’ Plant Physiology 171, No. 4: 2317-2330. doi: 10.1104/pp.16.00652.
  • Welchen E., Schmitz J., Fuchs P., García L., Wagner S., Wienstroer J., Schertl P., Braun H., Schwarzländer M., Gonzalez D., Maurino V. . ‘D-lactate dehydrogenase links methylglyoxal degradation and electron transport through cytochrome.’ Plant Physiology 172, No. 2: 901-912. doi: 10.1104/pp.16.01174.
  • Demaurex N., Schwarzländer M. . ‘Mitochondrial Flashes: Dump Superoxide and Dance with Protons Now.’ Antioxidants and Redox Signaling 25, No. 9: 550-551. doi: 10.1089/ars.2016.6819.
  • Fuchs R., Kopischke M., Klapprodt C., Hause G., Meyer A., Schwarzländer M., Fricker M., Lipka V. . ‘Immobilized subpopulations of leaf epidermal mitochondria mediate PENETRATION2-dependent pathogen entry control in arabidopsis.’ Plant Cell 28, No. 1: 130-145. doi: 10.1105/tpc.15.00887.
  • Wagner S., De Bortoli S., Schwarzländer M., Szabò I. . ‘Regulation of mitochondrial calcium in plants versus animals.’ Journal of Experimental Botany 67, No. 13: 3809-3829. doi: 10.1093/jxb/erw100.
  • Morgan B., Schwarzländer M. . „Fluoreszierende Proteinsensoren für die Redoxregulation in lebenden Zellen.“ BioSpektrum 22, No. 3: 260-263. doi: 10.1007/s12268-016-0683-2.
  • Huang S., Van Aken O., Schwarzländer M., Belt K., Millar A. . ‘The roles of mitochondrial reactive oxygen species in cellular signaling and stress response in plants.’ Plant Physiology 171, No. 3: 1551-1559. doi: 10.1104/pp.16.00166.
  • Breckwoldt M., Armoundas A., Aon M., Bendszus M., O'Rourke B., Schwarzländer M., Dick T., Kurz F. . ‘Mitochondrial redox and pH signaling occurs in axonal and synaptic organelle clusters.’ Scientific Reports 6, No. null. doi: 10.1038/srep23251.
  • Schwarzländer M., Dick T., Meyer A., Morgan B. . ‘Dissecting redox biology using fluorescent protein sensors.’ Antioxidants and Redox Signaling 24, No. 13: 680-712. doi: 10.1089/ars.2015.6266.
  • Wagner S., Nietzel T., Aller I., Costa A., Fricker M., Meyer A., Schwarzländer M. . ‘Analysis of plant mitochondrial function using fluorescent protein sensors.’ Methods in Molecular Biology 2015: 241-252. doi: 10.1007/978-1-4939-2639-8_17.
  • Riemer J., Schwarzländer M., Conrad M., Herrmann J. . ‘Thiol switches in mitochondria: Operation and physiological relevance.’ Biological Chemistry 396, No. 5: 465-482. doi: 10.1515/hsz-2014-0293.
  • Moseler A., Aller I., Wagner S., Nietzel T., Przybyla-Toscano J., Mühlenhoff U., Lill R., Berndt C., Rouhier N., Schwarzländer M., Meyer A. . ‘The mitochondrial monothiol glutaredoxin S15 is essential for iron-sulfur protein maturation in Arabidopsis thaliana.’ Proceedings of the National Academy of Sciences of the United States of America 112, No. 44: 13735-13740. doi: 10.1073/pnas.1510835112.
  • Kosmacz M., Parlanti S., Schwarzländer M., Kragler F., Licausi F., Van Dongen J. . ‘The stability and nuclear localization of the transcription factor RAP2.12 are dynamically regulated by oxygen concentration.’ Plant, Cell and Environment 38, No. 6: 1094-1103. doi: 10.1111/pce.12493.
  • Wagner S., Behera S., De Bortoli S., Logan D., Fuchs P., Carraretto L., Teardo E., Cendron L., Nietzel T., Füßl M., Doccula F., Navazio L., Fricker M., Van Aken O., Finkemeier I., Meyer A., Szabò I., Costa A., Schwarzländer M. . ‘The EF-hand Ca2+ binding protein MICU choreographs mitochondrial Ca2+ dynamics in arabidopsis.’ Plant Cell 27, No. 11: 3190-3212. doi: 10.1105/tpc.15.00509.
  • Breckwoldt M., Pfister F., Bradley P., Marinković P., Williams P., Brill M., Plomer B., Schmalz A., St Clair D., Naumann R., Griesbeck O., Schwarzländer M., Godinho L., Bareyre F., Dick T., Kerschensteiner M., Misgeld T. . ‘Multiparametric optical analysis of mitochondrial redox signals during neuronal physiology and pathology in vivo.’ Nature Medicine 20, No. 5: 555-560. doi: 10.1038/nm.3520.
  • Schaedler T., Thornton J., Kruse I., Schwarzländer M., Meyer A., Van Veen H., Balk J. . ‘A conserved mitochondrial ATP-binding cassette transporter exports glutathione polysulfide for cytosolic metal cofactor assembly.’ Journal of Biological Chemistry 289, No. 34: 23264-23274. doi: 10.1074/jbc.M114.553438.
  • Braun H., Binder S., Brennicke A., Eubel H., Fernie A., Finkemeier I., Klodmann J., König A., Kühn K., Meyer E., Obata T., Schwarzländer M., Takenaka M., Zehrmann A. . ‘The life of plant mitochondrial complex I.’ Mitochondrion 19, No. null: 295-313. doi: 10.1016/j.mito.2014.02.006.
  • El Zawily A., Schwarzländer M., Finkemeier I., Johnston I., Benamar A., Cao Y., Gissot C., Meyer A., Wilson K., Datla R., Macherel D., Jones N., Logan D. . ‘FRIENDLY regulates mitochondrial distribution, fusion, and quality control in Arabidopsis.’ Plant Physiology 166, No. 2: 808-828. doi: 10.1104/pp.114.243824.
  • Littlejohn G., Meckel T., Schwarzländer M., Costa A. . ‘Functional imaging in living plants—cell biology meets physiology.’ Frontiers in Plant Science 5, No. null. doi: 10.3389/fpls.2014.00740.
  • Schwarzländer M., Wagner S., Ermakova Y., Belousov V., Radi R., Beckman J., Buettner G., Demaurex N., Duchen M., Forman H., Fricker M., Gems D., Halestrap A., Halliwell B., Jakob U., Johnston I., Jones N., Logan D., Morgan B., Müller F., Nicholls D., Remington S., Schumacker P., Winterbourn C., Sweetlove L., Meyer A., Dick T., Murphy M. . ‘The 'mitoflash' probe cpYFP does not respond to superoxide.’ Nature 514, No. 7523: E12-E14. doi: 10.1038/nature13858.
  • Schwarzländer M., Finkemeier I. . ‘Mitochondrial energy and redox signaling in plants.’ Antioxidants and Redox Signaling 18, No. 16: 2122-2144. doi: 10.1089/ars.2012.5104.
  • Schwarzländer M., Logan D., Johnston I., Jones N., Meyer A., Fricker M., Sweetlove L. . ‘Pulsing of membrane potential in individual mitochondria: A stress-induced mechanism to regulate respiratory bioenergetics in Arabidopsis.’ Plant Cell 24, No. 3: 1188-1201. doi: 10.1105/tpc.112.096438.
  • Wirtz M., Beard K., Lee C., Boltz A., Schwarzländer M., Fuchs C., Meyer A., Heeg C., Sweetlove L., Ratcliffe R., Hell R. . ‘Mitochondrial cysteine synthase complex regulates O-acetylserine biosynthesis in plants.’ Journal of Biological Chemistry 287, No. 33: 27941-27947. doi: 10.1074/jbc.M112.372656.
  • Schwarzländer M., König A., Sweetlove L., Finkemeier I. . ‘The impact of impaired mitochondrial function on retrograde signalling: A meta-analysis of transcriptomic responses.’ Journal of Experimental Botany 63, No. 4: 1735-1750. doi: 10.1093/jxb/err374.
  • Schwarzländer M., Murphy M., Duchen M., Logan D., Fricker M., Halestrap A., Müller F., Rizzuto R., Dick T., Meyer A., Sweetlove L. . ‘Mitochondrial 'flashes': A radical concept repHined.’ Trends in Cell Biology 22, No. 10: 503-508. doi: 10.1016/j.tcb.2012.07.007.
  • Schwarzländer M., Logan D., Fricker M., Sweetlove L. . ‘The circularly permuted yellow fluorescent protein cpYFP that has been used as a superoxide probe is highly responsive to pH but not superoxide in mitochondria: Implications for the existence of superoxide 'flashes'.’ Biochemical Journal 437, No. 3: 381-387. doi: 10.1042/BJ20110883.
  • Williams T., Poolman M., Howden A., Schwarzlander M., Fell D., Ratcliffe R., Sweetlove L. . ‘A Genome-scale metabolic model accurately predicts fluxes in central carbon metabolism under stress conditions.’ Plant Physiology 154, No. 1: 311-323. doi: 10.1104/pp.110.158535.
  • Schwarzländer M., Fricker M., Sweetlove L. . ‘Monitoring the in vivo redox state of plant mitochondria: Effect of respiratory inhibitors, abiotic stress and assessment of recovery from oxidative challenge.’ Biochimica et Biophysica Acta - Bioenergetics 1787, No. 5: 468-475. doi: 10.1016/j.bbabio.2009.01.020.
  • Marty L., Siala W., Schwarzländer M., Fricker M., Wirtz M., Sweetlove L., Meyer Y., Meyer A., Reichheld J., Hell R. . ‘The NADPH-dependent thioredoxin system constitutes a functional backup for cytosolic glutathione reductase in Arabidopsis.’ Proceedings of the National Academy of Sciences of the United States of America 106, No. 22: 9109-9114. doi: 10.1073/pnas.0900206106.
  • Lehmann M., Schwarzländer M., Obata T., Sirikantaramas S., Burow M., Olsen C., Tohge T., Fricker M., Møller B., Fernie A., Sweetlove L., Laxa M. . ‘The metabolic response of Arabidopsis roots to oxidative stress is distinct from that of heterotrophic cells in culture and highlights a complex relationship between the levels of transcripts, metabolites, and flux.’ Molecular Plant 2, No. 3: 390-406. doi: 10.1093/mp/ssn080.
  • Forner J., Hölzle A., Jonietz C., Thuss S., Schwarzländer M., Weber B., Meyer R., Binder S. . ‘Mitochondrial mRNA polymorphisms in different Arabidopsis accessions.’ Plant Physiology 148, No. 2: 1106-1116. doi: 10.1104/pp.108.126201.
  • Morgan M., Lehmann M., Schwarzländer M., Baxter C., Sienkiewicz-Porzucek A., Williams T., Schauer N., Fernie A., Fricker M., Ratcliffe R., Sweetlove L., Finkemeier I. . ‘Decrease in manganese superoxide dismutase leads to reduced root growth and affects tricarboxylic acid cycle flux and mitochondrial redox homeostasis.’ Plant Physiology 147, No. 1: 101-114. doi: 10.1104/pp.107.113613.
  • Schwarzländer M., Fricker M., Müller C., Marty L., Brach T., Novak J., Sweetlove L., Hell R., Meyer A. . ‘Confocal imaging of glutathione redox potential in living plant cells.’ Journal of Microscopy 231, No. 2: 299-316. doi: 10.1111/j.1365-2818.2008.02030.x.