Allgemeines Physikalisches Kolloquium im Sommersemester 2013
Ort: 48149 Münster, Wilhelm-Klemm-Str. 10, IG I, HS 2,
Zeit: Donnerstag, 27.06.2013 16:00 Uhr c.t.
Kolloquiums-Kaffee ab 15:45 Uhr vor dem Hörsaal
Quantitative digital holographic microscopy: Methods for multifunctional biophysical analysis of living cells and tissue
Dr. Björn Kemper, Centrum für Biomedizinische Optik und Photonik, Universität Münster
Digital holographic microscopy (DHM) provides contact-less quantitative phase contrast imaging for the
detection of optical path length changes with accuracy down to several nanometers. The reconstruction of
digitally captured holograms is performed numerically based on the principles of wave-optics. Thus, multifocus imaging of specimens in different layers is achieved from a single digital hologram. The evaluation of quantitative DHM phase images allows monitoring of shape and thickness changes, 2D object tracking and
image segmentation. Furthermore, observation of dynamic processes without mechanical focus realignment and the determination of axial sample positions are enabled. In DHM, the interaction with the sample is minimized as the method is stain-free and the specimen is only exposed to low light intensities. Thus, minimally invasive quantitative imaging of biological tissue and long-term observation of living cell cultures are achieved. Moreover, the cellular refractive index, which is related to intracellular solute concentrations and tissue density, and the cellular dry mass are available. After an introduction to different DHM principles for quantitative live cell imaging, results from investigations on toxin and drug mediated cell alterations, cell swelling kinetics, cellular growth and cell motility are presented. In addition, it is shown that DHM is capable of quantifying inflammation in dissected tissue, wound healing in-vitro and cancer cell migration in 3D tissue models.
Einladende: Prof. Dr. Cornelia Denz
Im Auftrag der Hochschullehrer des Fachbereichs Physik
Prof. Dr. Nikos Doltsinis