Institute for Evolution and Biodiversity
D-48149 Münster, Germany
Tel.: +49 251/83-21016
Map NTSR: Maping non-targeted-site resistance to herbicides in Spirodela polyrhiza
My PhD project is focused on the identification of genes involved in conferring non-targeted-site resistance in plants. To answer this question, I am applying a forward genetics approach on the duckweed Spirodela polyrhiza. The first step of my project includes an herbicide toxicity assay. Here, I cultivate 200 genotypes of Spirodela polyrhiza under herbicide stress conditions with the aim to identify the most herbicide resistant genotypes. In the second phase of my thesis, I am going to identify gene loci that are associated with this herbicide tolerance. This will be done in correlation-based computational approaches like genome wide association studies (GWAS) or coexpression networks. Finally, the involvement of the identified gene loci in conferring herbicide resistance will be examined in a reverse genetics approach. This will involve gene overexpression studies or gene knock-outs via the CRISPR/Cas9 platform.