Hospital and Environmental Hygiene
Institute of Hygiene
D-48149 Münster, Germany
Surveillance and characterisation of antimicrobial resistance harbouring plasmids
Gram-negative multidrug resistant bacteria (MDRB) are a global health problem and economic burden. Until recently research has focussed on antimicrobial resistance (AMR) spread through whole bacteria transmission while the role of AMR plasmids has rarely been addressed yet.
The aim of this study is to analyse clinical relevant AMR genes on plasmids and monitor AMR transmission via horizontal gene transfer (HGT). We will monitor stability and movement activity of AMR genes in clinical isolates with follow-up samples using long-read whole genome sequencing (lrWGS). In addition, in vitro experiments will be performed with clinical isolates to test stability of selected AMR harbouring plasmids under different culture conditions. These include sub inhibitory antibiotic concentrations and different pH, for example, to mimic
gastro-intestinal conditions and to induce stress. In another in vitro experiment, it is planned to recover the AMR of a MDRB with a CRISPR-Cas based approach and perform co-cultivation experiments afterwards to monitor possible HGT of AMR genes. The evaluation of in vitro experiments will primarily be done using lrWGS.
The project results will enhance the knowledge about AMR transmission and should generate new ideas for strategies to prevent spread of AMR in the hospital setting.