© EIMI

Intravitale molekulare Bildgebung (Prof. Friedemann Kiefer)

Uns faszinieren die gestalt-gebenden oder morphogenetischen Prozesse, welche während der Bildung der Gefäßsysteme, aber auch ihrer Aufrechterhaltung oder während Krankheitsprozessen am Werk sind. Ein besonderer Schwerpunkt liegt für uns auf den Gefäßen des lymphatischen Systems. Ein zentrales Element ist dabei das Wechselspiel aus Blutgefäßen, Gewebesauerstoffversorgung (oder Unterversorgung, auch als Hypoxie bezeichnet) und weißen Blutkörperchen und seine Bedeutung für die Organfunktion in verschiedenen physiologischen und pathologischen Situationen. Unsere Arbeit ruht in ganz entscheidendem Maße auf dem intensiven Einsatz hochmoderner mikroskopischer Techniken aber auch von Reporter-Modellsystemen, die es uns erlauben, diese Dreierbeziehung im Zusammenhang des intakten Gewebes oder sogar zeitlich aufgelöst im lebenden Organismus zu verfolgen.

  • News & Themen aus der Forschung

  • Projekte

  • Team

  • Publikationen

    2019

    Ghaffari A, Hoskin V, Turashvili G, Varma S, Mewburn J, Mullins G, Greer PA, Kiefer F, Day AG, Madarnas Y, SenGupta S, Elliott BE. Intravital imaging reveals systemic ezrin inhibition impedes cancer cell migration and lymph node metastasis in breast cancer. Breast Cancer Res 2019;21: 12. Abstract
    Hunter MC, Teijeira A, Montecchi R, Russo E, Runge P, Kiefer F, Halin C. Dendritic Cells and T Cells Interact Within Murine Afferent Lymphatic Capillaries. Front. Immunol. 2019;10: 11 pp. Abstract

    2018

    Bovay E, Sabine A, Prat-Luri B, Kim S, Son K, Willrodt A-H, Olsson C, Halin C, Kiefer F, Betsholtz C, Jeon NL, Luther SA, Petrova TV. Multiple roles of lymphatic vessels in peripheral lymph node development. J Exp Med 2018;215: 2760-2777. Abstract
    Dierkes C, Scherzinger A, Kiefer F. Three-Dimensional Visualization of the Lymphatic Vasculature. Methods Mol Biol 2018;1846: 1-18. Abstract
    Frye M, Taddei A, Dierkes C, Martinez-Corral I, Fielden M, Ortsater H, Kazenwadel J, Calado DP, Ostergaard P, Salminen M, He L, Harvey NL, Kiefer F, Makinen T. Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program. Nat Commun 2018;9: 1511. Abstract
    Hägerling R, Hoppe E, Dierkes C, Stehling M, Makinen T, Butz S, Vestweber D, Kiefer F. Distinct roles of VE‐cadherin for development and maintenance of specific lymph vessel beds. EMBO J 2018: e98271. Abstract
    Orlich M, Kiefer F. A qualitative comparison of ten tissue clearing techniques. Histol Histopathol 2018;33: 181-199. Abstract
    Seifert R, Kuhlmann MT, Eligehausen S, Kiefer F, Hermann S, Schafers M. Molecular imaging of MMP activity discriminates unstable from stable plaque phenotypes in shear-stress induced murine atherosclerosis. PLoS One 2018;13: e0204305. Abstract

    2017

    Hagerling R, Drees D, Scherzinger A, Dierkes C, Martin-Almedina S, Butz S, Gordon K, Schafers M, Hinrichs K, Ostergaard P, Vestweber D, Goerge T, Mansour S, Jiang X, Mortimer PS, Kiefer F. VIPAR, a quantitative approach to 3D histopathology applied to lymphatic malformations. JCI Insight 2017;2Abstract
    Karpanen T, Padberg Y, van de Pavert SA, Dierkes C, Morooka N, Peterson-Maduro J, van de Hoek G, Adrian M, Mochizuki N, Sekiguchi K, Kiefer F, Schulte D, Schulte-Merker S. An Evolutionarily Conserved Role for Polydom/Svep1 During Lymphatic Vessel Formation. Circ Res 2017;120: 1263-1275. Abstract
    Seifert R, Scherzinger A, Kiefer F, Hermann S, Jiang X, Schafers MA. Statistical Permutation-based Artery Mapping (SPAM): a novel approach to evaluate imaging signals in the vessel wall. BMC Med Imaging 2017;17: 36. Abstract
    Weis V, Konigsberger S, Amler S, Wienands J, Kiefer F. Unperturbed Immune Function despite Mutation of C-Terminal Tyrosines in Syk Previously Implicated in Signaling and Activity Regulation. Mol Cell Biol 2017;37Abstract

    2016

    Erapaneedi R, Belousov VV, Schafers M, Kiefer F. A novel family of fluorescent hypoxia sensors reveal strong heterogeneity in tumor hypoxia at the cellular level. Embo J 2016;35: 102-113. Abstract
    Scherzinger A, Kleene F, Dierkes C, Kiefer F, Hinrichs K, Jiang X. Automated Segmentation of Immunostained Cell Nuclei in 3D Ultramicroscopy Images. In: Pattern Recognition - 38th German Conference, GCPR 2016, Hannover, Germany, Sept. 12-15, 2016;Springer: 105-116. Abstract

    2015

    Bălan M, Kiefer F. A novel model for ectopic, chronic, intravital multiphoton imaging of bone marrow vasculature and architecture in split femurs. IntraVital 2015;4: e1066949. Abstract
    Helker CSM, Schuermann A, Pollmann C, Chng SC, Kiefer F, Reversade B, Herzog W. The hormonal peptide Elabela guides angioblasts to the midline during vasculogenesis. Elife 2015;4: e06726. Abstract

    2014

    Born G, Breuer D, Wang S, Rohlmann A, Coulon P, Vakili P, Reissner C, Kiefer F, Heine M, Pape H-C, Missler M. Modulation of synaptic function through the alpha-neurexin-specific ligand neurexophilin-1. Proc Natl Acad Sci U S A 2014;111: E1274-83. Abstract
    D'Amico G, Korhonen EA, Anisimov A, Zarkada G, Holopainen T, Hagerling R, Kiefer F, Eklund L, Sormunen R, Elamaa H, Brekken RA, Adams RH, Koh GY, Saharinen P, Alitalo K. Tie1 deletion inhibits tumor growth and improves angiopoietin antagonist therapy. J Clin Invest 2014;124: 824-834. Abstract
    Dartsch N, Schulte D, Hagerling R, Kiefer F, Vestweber D. Fusing VE-Cadherin to alpha-Catenin Impairs Fetal Liver Hematopoiesis and Lymph but Not Blood Vessel Formation. Mol Cell Biol 2014;34: 1634-1648. Abstract
    Pollmann C, Hägerling R, Kiefer F. Visualization of lymphatic vessel development, growth, and function. Adv Anat Embryol Cell Biol 2014;214: 167-186. Abstract

    2013

    Hägerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams RH, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F. A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy. EMBO J 2013;32: 629-644. Abstract
    Pollmann C, Hägerling R, Andreas M, Kiefer F. Ultramikroskopische Darstellung von Lymphgefäßen während der Fötalentwicklung und im Erwachsenenstadium der Maus. LymphForsch 2013;17: 6-12.
    Wang L, Benedito R, Bixel MG, Zeuschner D, Stehling M, Savendahl L, Haigh JJ, Snippert H, Clevers H, Breier G, Kiefer F, Adams RH. Identification of a clonally expanding haematopoietic compartment in bone marrow. EMBO J 2013;32: 219-230. Abstract

    2012

    Königsberger S, Prodöhl J, Stegner D, Weis V, Andreas M, Stehling M, Schumacher T, Böhmer R, Thielmann I, van Eeuwijk JMM, Nieswandt B, Kiefer F. Altered BCR signalling quality predisposes to autoimmune disease and a pre-diabetic state. EMBO J 2012;31: 3363-3374. Abstract
    Sabine A, Agalarov Y, Maby-El Hajjami H, Jaquet M, Hägerling R, Pollmann C,Bebber D, Pfenniger A, Miura N, Dormond O, Calmes JM, Adams RH, Mäkinen T, Kiefer F, Kwak BR, Petrova TV. Mechanotransduction, PROX1, and FOXC2 Cooperate to Control Connexin37 and Calcineurin during Lymphatic-Valve Formation. Dev Cell 2012: 430-45. Abstract

    2011

    Hägerling R, Pollmann C, Kremer L, Andresen V, Kiefer F. Intravital two-photon microscopy of lymphatic vessel development and function using a transgenic Prox1 promoter-directed mOrange2 reporter mouse. Biochem Soc Trans 2011;39: 1674-1681. Abstract
    Kiefer F, Siekmann AF. The role of chemokines and their receptors in angiogenesis. Cell Mol Life Sci 2011;68: 2811-2830. Abstract
    Neuhaus B, Buhren S, Bock B, Alves F, Vogel WF, Kiefer F. Migration inhibition of mammary epithelial cells by Syk is blocked in the presence of DDR1 receptors. Cell Mol Life Sci 2011;68: 3757-70. Abstract
    Schulte D, Kuppers V, Dartsch N, Broermann A, Li H, Zarbock A, Kamenyeva O, Kiefer F, Khandoga A, Massberg S, Vestweber D. Stabilizing the VE-cadherin-catenin complex blocks leukocyte extravasation and vascular permeability. EMBO J 2011: 4157-70. Abstract

    2010

    Böhmer R, Neuhaus B, Bühren S, Zhang D, Stehling M, Böck B, Kiefer F. Regulation of developmental lymphangiogenesis by Syk(+) leukocytes. Dev Cell 2010;18: 437-449. Abstract
    Patzak IM, Königsberger S, Suzuki A, Mak TW, Kiefer F. HPK1 competes with ADAP for SLP-76 binding and via Rap1 negatively affects T-cell adhesion. Eur J Immunol 2010;40: 3220-3225. Abstract

    2009

    Winderlich M, Keller L, Cagna G, Broermann A, Kamenyeva O, Kiefer F, Deutsch U, Nottebaum AF, Vestweber D. VE-PTP controls blood vessel development by balancing Tie-2 activity. J Cell Biol 2009;185: 657-671. Abstract

    2007

    Arnold R, Frey CR, Muller W, Brenner D, Krammer PH, Kiefer F. Sustained JNK signaling by proteolytically processed HPK1 mediates IL-3 independent survival during monocytic differentiation. Cell Death Differ 2007;14: 568-575. Abstract

    2005

    Arnold R, Patzak IM, Neuhaus B, Vancauwenbergh S, Veillette A, Van Lint J, Kiefer F. Activation of hematopoietic progenitor kinase 1 involves relocation, autophosphorylation, and transphosphorylation by protein kinase D1. Mol Cell Biol 2005;25: 2364-2383. Abstract