Cells‘ ability to sense mechanical information is, to a large degree, mediated by macromolecular structures which mediate the adhesion of cells to the extracellular matrix (ECM) or to each other. The structures which mediate cell-ECM adhesion are based on integrin receptors and are called focal adhesions (FAs); the interaction between cells is realized by cadherin-based structures including adherens junctions (AJ) and desmosomes (DES). Even though the central role of these complexes for many mechanical processes during development and adult life has been appreciated for long time, it is still unknown how mechanical force propagate across cell-matrix and cell-cell adhesion on the molecular level. The combination of single-molecule calibrated biosensors with adanced microscopy allows us to adress these questions with unprecedented sensitivity and resolution.
Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA. Nature. 2010 Jul 8; 466:263-266. >>
Hoffman BD, Grashoff C, Schwartz MA. Nature. 2011 Jul 20; 475:316-323. >>
Austen K, Ringer P, Mehlich A, Chrostek-Grashoff A, Kluger C, Klingner C, Sabass B, Zent R, Rief M, Grashoff C. Nat Cell Biol. 2015 Dec; 17(12):1597-1606. [Epub 2015 Nov 2] >>
Ringer P, Weißl A, Cost AL, Freikamp A, Sabass B, Mehlich A, Tramier M, Rief M, Grashoff C. Nature Methods 2017 Nov; 14(11):1090-1096. [Epub 2017 Sep 18] >>