Research Foci
- Mechanisms of exo- and endocyosis, with particular emphasis on synaptic vesicle recycling
- cell physiology of synaptic transmission using electrophysiology and high-resolution imaging techniques
Further Affiliation at the University of Münster
CV
Academic Education
- PhD in Physics (Dr. rer. nat.), University of Göttingen
- Degree Student with Prof. Erwin Neher, Max Planck Institute for Biophysical Chemistry, Göttingen
- Studies of Biology and Physics, Universities of Hamburg and Bonn
Positions
- Head of Institute of Medical Physics and Biophysics, University of Münster (WWU)
- Professor (W3) of Medical Physics and Biophysics, Medical Faculty, University of Münster (WWU)
- Independent Research Group Leader, MPI Biophysical Chemistry, Göttingen
- Postdoc with Prof. Erwin Neher, Department of Membrane Biophysics, MPI Biophysical Chemistry, Göttingen
- Guest Researcher, Department of Molecular Cellular Physiology, Stanford Medical School, California, USA
Honors
- PhD Fellowship – Boehringer Ingelheim Fonds (BIF)
- Grant from the Human Frontier Science Program Organisation – International Human Frontier Science Program Organization (HFSP)
External Functions
- Member of the Neuroscience Graduate Study Programme, Max Planck Institute and the University, Göttingen
- Member of the Society for Neuroscience, Washington D.C., USA
- Member of the American Biophysical Society, Bethesda, MD, USA
- Member of the German Society of Physiology, Rostock
Appointments
- Offer, Professorship at the Medical School, Yale University, New Haven, CT, USA (declined)
Yale University – rejected - Offer, Professorship, Cluster of Excellence Macromolecular Complexes, University of Frankfurt (declined)
Goethe University Frankfurt am Main, Cluster of Excellence Macromolecular Complexes – rejected
Projects
- CRC 1348: Dynamic cellular interfaces - formation and function ( – )
Main DFG-Project Hosted at the University of Münster: DFG - Collaborative Research Centre | Project Number: SFB 1348/2 - CRC 1348 A02 - Local signaling for tight coupling of synaptic exo- and endocytosis ( – )
Subproject in DFG-Joint Project Hosted at the University of Münster: DFG - Collaborative Research Centre | Project Number: SFB 1348/2 A02 - CRC 1348 - Dynamic cellular interfaces: formation and function ( – )
Main DFG-Project Hosted at the University of Münster: DFG - Collaborative Research Centre | Project Number: SFB 1348/1 - CRC 1348 A02 - Local signaling for tight coupling of synaptic exo- and endocytosis ( – )
Subproject in DFG-Joint Project Hosted at the University of Münster: DFG - Collaborative Research Centre | Project Number: SFB 1348/1 A02 - EVO-CELL – KMU-innovativ-21: EVO-CELL - Entwicklung einer multiparametrischen Zellanalysetechnologie für die Erforschung und Entwicklung zellbasierter Therapien ( – )
Participation in Federally Funded Joint Project: Federal Ministry of Research, Technology and Space | Project Number: 031B0654B - EXC 1003 A3 - Functional membrane domains in neuronal and non-neuronal compartmentalization ( – )
Subproject in DFG-Joint Project Hosted at the University of Münster: DFG - Cluster of Excellence | Project Number: EXC1003/1 - EXC 1003 FF-2017-10 - Biohybrid neurosynaptic chips interfaced with nanostructured, integrated optics ( – )
Subproject in DFG-Joint Project Hosted at the University of Münster: DFG - Cluster of Excellence | Project Number: FF-2017-10 - EXC 1003 FF-2015-05 – Direct neuronal Interfacing on functionalized microstructured coverslips for imaging synapse development and plasticity ( – )
Subproject in DFG-Joint Project Hosted at the University of Münster: DFG - Cluster of Excellence - CRC 629 A17 - Lipid- und Protein-Sortierung und Recyclierung während der synaptischen Übertragung ( – )
Subproject in DFG-Joint Project Hosted at the University of Münster: DFG - Collaborative Research Centre | Project Number: SFB629A017 - VesDyn – Synaptic vesicle acidification and refilling dynamics ( – )
EU-Project Hosted at University the of Münster: EC FP 7 - Marie Curie Actions - Intra-European Fellowships - CiM-IMPRS – Cells in Motion (CiM) Graduate School and International Max Planck Research School - Molecular Biomedicine (since )
Individual Granted Project: Federal Ministry of Culture and Science of the Federal State of North Rhine-Westphalia - EuroSynapse - Spatio-temporal organization of the synaptic membrane for synaptic vesicle protein recycling ( – )
Individual Granted Project: DFG - Individual Grants Programme | Project Number: KL 1334/2-1 - Schaltbare Nanopartikel als Wirkstoffträger ( – )
Own Resources Project - Visualization of the fate of synaptic vesicle proteins post fusion ( – )
Individual Granted Project: DFG - Individual Grants Programme | Project Number: KL 1334/1-1
- CRC 1348: Dynamic cellular interfaces - formation and function ( – )
Publications
- Schnieder, M., Niemann, A., Hüve, J., and Klingauf, J. . “Collimating three-axicon zoom system for interferometric Bessel beam side lobe cancellation.” Optical and Quantum Electronics, № 56 716. doi: 10.1007/s11082-023-06229-y.
- Singh, A, Thale, S, Leibner, T, Lamparter, L, Ricker, A, Nüsse, H, Klingauf, J, Galic, M, Ohlberger, M, and Matis, M. . “Dynamic interplay of microtubule and actomyosin forces drive tissue extension.” Nature Communications, № 15 (1): 3198–3198. doi: 10.1038/s41467-024-47596-8.
- Schnieder, M., Voß, F., Hüve, J., and Klingauf, J. . “Combining Dual-View Plane Illumination Microscopy with Bessel Beams.” contributed to the Focus on Microscopy, Porto
- Schnieder, M., Niemann, A., Hüve, J., and Klingauf, J. . “Power Efficient 3-Axicon System for Generation of Tuneable Bessel Beams.” contributed to the Focus on Microscopy, Online
- Goossen-Schmidt, N.C., Schnieder, M., Hüve, J., and Klingauf, J. . “Switching behaviour of dSTORM dyes in glycerol-containing buffer.” Scientific Reports, № 10 doi: 10.1038/s41598-020-70335-0.
- Kaspar, C, Lehrich, J, Ivanenko, A, Klingauf, J, and Pernie, W. . “Integrated photonics chip for neural activity investigation.” Optogenetics and Optical Manipulation, № 11227 doi: 10.1117/12.2546183.
- Dlugos, C.P., Picciotto, C., Lepa, C., Krakow, M., Stober, A., Eddy, M.L., Weide, T., Jeibmann, A., P., Krahn M., Van Marck, V., and Klingauf, J. Ricker A. Wedlich-Soldner R. Pavenstadt H. Klambt C. George B. . “Nephrin Signaling Results in Integrin beta1 Activation.” Journal of the American Society of Nephrology, № 30 (6): 1006–1019. doi: 10.1681/ASN.2018040362.
- Schmidt, N.C., Schnieder, M., Hüve, J., and Klingauf, J. . “Intrinsic Refractive Index Matched 3D dSTORM with Two Objectives.” contribution to the Focus On Microscopy, London, Vereinigtes Königreich
- Bergemann, I, Saha, T, Lamparter, L, Rathmann, I, Grill, D, Golbach, L, Rasch, C, Keller, U.Trappmann B, Matis, M, Gerke, V, Klingauf, J, and Galic, M. . “Mechanochemical self-organization determines search pattern in migratory cells.” Nature Physics, № 15 doi: 10.1038/s41567-019-0505-9.
- Schmidt, Nora C Kahms Martin, and Hüve, Jana Klingauf Jürgen. . “Intrinsic refractive index matched 3D dSTORM with two objectives: Comparison of detection techniques.” Scientific Reports, № 8 doi: 10.1038/s41598-018-31595-z.
- Rajappa, R., Gauthier-Kemper, A., Böning, D., Hüve, J., and Klingauf, J. . “Synaptophysin 1 Clears Synaptobrevin 2 from the Presynaptic Active Zone to Prevent Short-Term Depression.” Cell Reports, № 14 (6): 1369–1381. doi: 10.1016/j.celrep.2016.01.031.
- Schmidt, NC, Hüve, J, and Klingauf, J. . “3D Pointillism Microscopy setup with two objectives.” in Verhandlungen der Deutschen Physikalischen Gesellschaft, Vol. 51 of 4, edited by DrBernhard Nunner.
- Schmidt, NC, Hüve, J, and Klingauf, J. . “Dual-Objective Pointillism Microscopy Setup with Interferometric and Astigmatic Detection.” in Biophysical Journal, Vol. 108 of 2 Elsevier.
- Gauthier-Kemper, A, Rajappa, R, Wiemhöfer, M, Thiel, CS, Hüve, J, and Klingauf, J. . “Syp1 Acts as a Clearance Factor for Syb2 at the Presynapse.” contribution to the Biophysical Society 58th annual meeting, San Francisco Amsterdam: Elsevier.
- Trahe, J, Hüve, J, Selenschik, P, Glyvuk, N, Gauthier-Kemper, A, Piehler, J, and Klingauf, J. . “Guided Growth of Neurons on Micro-Structured Surfaces.” contribution to the Biophysical Society 58th annual meeting, San Francisco Amsterdam: Elsevier.
- Sinha, R, Ahmed, S, Jahn, R, and Klingauf, J. . “Two synaptobrevin molecules are sufficient for vesicle fusion in central nervous system synapses.” Proceedings of the National Academy of Sciences of the United States of America, № 108 (34): 14318–14323. doi: 10.1073/pnas.1101818108.
- Hua, YF, Sinha, R, Thiel, CS, Schmidt, R, Huve, J, Martens, H, Hell, SW, Egner, A, and Klingauf, J. . “A readily retrievable pool of synaptic vesicles.” Nature Neuroscience, № 14 (7): 833. doi: 10.1038/nn.2838.
- Sinha, R, Ahmed, S, Jahn, R, and Klingauf, J. . “Two synaptobrevin molecules are sufficient for vesicle fusion in central nervous system synapses.” Proceedings of the National Academy of Sciences of the United States of America, № 108 (34): 14318–14323.
- Groemer, TW, Thiel, CS, Holt, M, Riedel, D, Hua, Y, Hüve, J, Wilhelm, BG, and Klingauf, J. . “Amyloid precursor protein is trafficked and secreted via synaptic vesicles.” PloS one, № 6 (4): e18754.
- Hua, Y, Sinha, R, Thiel, CS, Schmidt, R, Hüve, J, Martens, H, Hell, SW, Egner, A, and Klingauf, J. . “A readily retrievable pool of synaptic vesicles.” Nature Neuroscience, № 14 (7): 833–835.
- Mothet, JP, Shi, T, Puyal, J, Klingauf, J, Sweedler, J, Jahn, R, and Martineau, M. . “VESICULAR TRANSPORT OF D-SERINE IN ASTROCYTES.” Glia, № 59 1: S34–S35.
- Boening, D, Groemer, TW, and Klingauf, J. . “Applicability of an EM-CCD for spatially resolved TIR-ICS.” Optics Express, № 18 (13): 13516–13528. doi: 10.1364/OE.18.013516.
- Glyvuk, N, Tsytsyura, Y, Geumann, C, D'Hooge, R, Huve, J, Kratzke, M, Baltes, J, Boening, D, Klingauf, J, and Schu, P. . “AP-1/sigma 1B-adaptin mediates endosomal synaptic vesicle recycling, learning and memory.” The EMBO Journal, № 29 (11): 1941–1941. doi: 10.1038/emboj.2010.96.
- Glyvuk, N, Tsytsyura, Y, Geumann, C, D'Hooge, R, Huve, J, Kratzke, M, Baltes, J, Boning, D, Klingauf, J, and Schu, P. . “AP-1/sigma 1B-adaptin mediates endosomal synaptic vesicle recycling, learning and memory.” The EMBO Journal, № 29 (8): 1318–1330. doi: 10.1038/emboj.2010.15.
- Glyvuk, N, Tsytsyura, Y, Geumann, C, D'Hooge, R, Huve, J, Kratzke, M, Baltes, J, Boening, D, Klingauf, J, and Schu, P. . “AP-1/sigma 1B-adaptin mediates endosomal synaptic vesicle recycling, learning and memory (vol 29, pg 1318, 2010).” The EMBO Journal, № 29 (11): 1941–1941.
- Boening, D, Groemer, TW, and Klingauf, J. . “Applicability of an EM-CCD for spatially resolved TIR-ICS.” Plant Methods, № 18 (13): 13516–13528. doi: 10.1364/OE.18.013516.
- Hua, Y, Sinha, R, Martineau, M, Kahms, M, and Klingauf, J. . “A common origin of synaptic vesicles undergoing evoked and spontaneous fusion.” Nature Neuroscience, № 13 (12): 1451–1453. doi: 10.1038/nn.2695.
- Glyvuk, N, Tsytsyura, Y, Geumann, C, D'Hooge, R, Hüve, J, Kratzke, M, Baltes, J, Boening, D, Böning, D, Klingauf, J, and Schu, P. . “AP-1/sigma1B-adaptin mediates endosomal synaptic vesicle recycling, learning and memory.” The EMBO Journal, № 29 (8): 1318–1330. doi: 10.1038/emboj.2010.15.
- Welzel, O, Boening, D, Stroebel, A, Reulbach, U, Klingauf, J, Kornhuber, J, and Groemer, TW. . “Determination of axonal transport velocities via image cross- and autocorrelation.” European Biophysics Journal, № 38 (7): 883–889. doi: 10.1007/s00249-009-0458-5.
- Medigeshi, GR, Krikunova, M, Radhakrishnan, K, Wenzel, D, Klingauf, J, and Schu, P. . “AP-1 membrane-cytoplasm recycling regulated by mu 1A-adaptin.” Traffic, № 9 (1): 121–132. doi: 10.1111/j.1600-0854.2007.00672.x.
- Jung, N, Wienisch, M, Gu, M, Rand, JB, Muller, SL, Krause, G, Jorgensen, EM, Klingauf, J, and Haucke, V. . “Molecular basis of synaptic vesicle cargo recognition by the endocytic sorting adaptor stonin 2.” Journal of Cell Biology, № 179 (7): 1497–1510. doi: 10.1083/jcb.200708107.
- Gulyas-Kovacs, A, de Wit, H, Milosevic, I, Kochubey, O, Toonen, R, Klingauf, J, Verhage, M, and Sorensen, JB. . “Munc18-1: Sequential interactions with the fusion machinery stimulate vesicle docking and priming.” Journal of Neuroscience, № 27 (32): 8676–8686.
- Tsytsyura, Y, Glyvuk, N, Riel, C, Baltes, J, Schu, P, and Klingauf, J. . “A role for the endosomal adaptor protein AP1 in synaptic vesicle cycling.” Journal of General Physiology, № 130 (1): 15a16a.
- Thiel, CS, Groemer, TW, Wienands, J, and Klingauf, J. . “A role for the F-actin binding protein mAbp1 in clathrin-mediated endocytosis.” Journal of General Physiology, № 130 (1): 15a15a.
- Klingauf, J. . “Coupling of exo- and endocytosis at CNS synapses.” Journal of General Physiology, № 130 (1): 4a4a.
- Henkel, AW, Dittrich, PS, Groemer, TW, Lemke, EA, Klingauf, J, Klafki, HW, Lewczuk, P, Esselmann, H, Schwille, P, Kornhuber, J, and Wiltfang, J. . “Immune complexes of auto-antibodies against A beta 1-42 peptides patrol cerebrospinal fluid of non-Alzheimer's patients.” Molecular Psychiatry, № 12 (6): 601–610. doi: 10.1038/sj.mp.4001947.
- Klingauf, J. . “Synaptic vesicle dynamics sans dynamin.” Neuron, № 54 (6): 857–858.
- Vanden, Berghe P, and Klingauf, J. . “Spatial organization and dynamic properties of neurotransmitter release sites in the enteric nervous system.” Neuroscience, № 145 (1): 88–99.
- Groemer, TW, and Klingauf, J. . “Synaptic vesicles recycling spontaneously and during activity belong to the same vesicle pool.” Nature Neuroscience, № 10 (2): 145–147. doi: 10.1038/nn1831.
- Kochubey, O, Majumdar, A, and Klingauf, J. . “Imaging clathrin dynamics in Drosophila melanogaster hemocytes reveals a role for actin in vesicle fission.” Traffic, № 7 (12): 1614–1627. doi: 10.1111/j.1600-0854.2006.00492.x.
- Takamori, S, Holt, M, Stenius, K, Lemke, EA, Gronborg, M, Riedel, D, Urlaub, H, Schenck, S, Brugger, B, Ringler, P, Muller, SA, Rammner, B, Grater, F, Hub, JS, De Groot, BL, Mieskes, G, Moriyama, Y, Klingauf, J, Grubmuller, H, Heuser, J, Wieland, F, and Jahn, R. . “Molecular anatomy of a trafficking organelle.” Cell, № 127 (4): 831–846. doi: 10.1016/j.cell.2006.10.030.
- Wienisch, M, and Klingauf, J. . “Vesicular proteins exocytosed and subsequently retrieved by compensatory endocytosis are nonidentical.” Nature Neuroscience, № 9 (8): 1019–1027. doi: 10.1038/nn1739.
- Toonen, RF, Kochubey, O, de Wit, H, Gulyas-Kovacs, A, Konijnenburg, B, Sorensen, JB, Klingauf, J, and Verhage, M. . “Dissecting docking and tethering of secretory vesicles at the target membrane.” The EMBO Journal, № 25 (16): 3725–3737. doi: 10.1038/sj.emboj.7601256.
- Vanden, Berghe P, and Klingauf, J. . “Synaptic vesicles in rat hippocampal boutons recycle to different pools in a use-dependent fashion.” Journal of Physiology, № 572 (3): 707–720. doi: 10.1113/jphysiol.2005.100842.
- Connert, S, Wienand, S, Thiel, C, Krikunova, M, Glyvuk, N, Tsytsyura, Y, Hilfiker-Kleiner, D, Bartsch, JW, Klingauf, J, and Wienands, J. . “SH3P7/mAbp1 deficiency leads to tissue and behavioral abnormalities and impaired vesicle transport.” The EMBO Journal, № 25 (8): 1611–1622.
- Diril, MK, Wienisch, M, Jung, N, Klingauf, J, and Haucke, V. . “Stonin 2 is an AP-2-dependent endocytic sorting adaptor for synaptotagmin internalization and recycling.” Developmental Cell, № 10 (2): 233–244. doi: 10.1016/j.devcel.2005.12.011.
- Lemke, EA, and Klingauf, J. . “Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity.” Journal of Neuroscience, № 25 (47): 11034–11044.
- Loerke, D, Wienisch, M, Kochubey, O, and Klingauf, J. . “Differential control of clathrin subunit dynamics measured with EW-FRAP microscopy.” Traffic, № 6 (10): 918–929. doi: 10.1111/j.1600-0854.2005.00329.x.
- Jordan, R, Lemke, EA, and Klingauf, J. . “Visualization of synaptic vesicle movement in intact synaptic boutons using fluorescence fluctuation spectroscopy.” Biophysical Journal, № 89 (3): 2091–2102. doi: 10.1529/biophysj.105.061663.
- Mueller, VJ, Wienisch, M, Nehring, RB, and Klingauf, J. . “Monitoring clathrin-mediated endocytosis during synaptic activity.” Journal of Neuroscience, № 24 (8): 2004–2012. doi: 10.1523/JNEUROSCI.4080-03.2004.
- Choi, S, Klingauf, J, and Tsien, RW. . “Fusion pore modulation as a presynaptic mechanism contributing to expression of long-term potentiation.” Philosophical Transactions of the Royal Society B: Biological Sciences, № 358 (1432): 695–705. doi: 10.1098/rstb.2002.1249.
- Lemke, EA, and Klingauf, J. . “Visualization of single synaptic vesicle dynamics in hippocampal boutons by two-photon laser-scanning microscopy.” Biophysical Journal, № 84 (2): 211A211A.
- Wienisch, M, and Klingauf, J. . “A slow endocytosis pathway in hippocampal synapses.” Biophysical Journal, № 82 (1): 616A617A.
- Bundy, A, Loerke, D, and Klingauf, J. . “A synaptosomal preparation for studying vesicle cycling by evanescent wave microscopy.” Biophysical Journal, № 82 (1): 615A.
- Wienisch, M, Muller, VJ, Nehring, RB, and Klingauf, J. . “Monitoring clathrin-mediated endocytosis in hippocampal synapses.” Biophysical Journal, № 82 (1): 10A10A.
- Jordan, R, and Klingauf, J. . “Vesicle dynamics in hippocampal boutons visualised by fluorescence spectroscopy.” European Journal of Neuroscience, № 12: 367–367.
- Bruns, D, Riedel, D, Klingauf, J, and Jahn, R. . “Quantal release of serotonin.” Neuron, № 28 (1): 205–220.
- Choi, S, Klingauf, J, and Tsien, RW. . “Postfusional regulation of cleft glutamate concentration during LTP at 'silent synapses'.” Nature Neuroscience, № 3 (4): 330–336.
- Jordan, R, and Klingauf, J. . “Vesicle dynamics in hippocampal boutons in culture visualized by fluorescence correlation spectroscopy.” Biophysical Journal, № 78 (1): 260A260A.
- Klingauf, J, Kavalali, ET, and Tsien, RW. . “Kinetics and regulation of fast endocytosis at hippocampal synapses.” Nature, № 394 (6693): 581–585. doi: 10.1038/29079.
Supervised Doctoral Study
Göllner, Bernd The Role of Giant Ankyrins in the Organization and Stabilization of the Synaptic Cytoskeleton at the Drosophila Neuromuscular Junction
Prof. Dr. Jürgen Klingauf
