In-vivo label free video microscopy

Many biological questions can only properly be answered in vivo with high temporal resolution. State-of-the-art observation technologies such as spinning-disk microscopy and other fluorescence-based imaging technologies suffer from poor temporal resolution with less than 1000 samples per second. By employing high speed video microscopy and laser deflection we are able to resolve these processes. In contrast to fluorescence, no labelling of the investigated objects, such as red blood cells in blood flow, is required.

The in-vivo investigation of blood flow and shear stress is a standard parameter in biological research on vasculatory systems. We combine state-of-the-art videomicroscopy with high speed cameras. By using correlative image evaluation with background correction we are able to raise the signal-to-noise ratio to levels that do not require an additional labelling, facilitating sample preparation.