© Budde

Topics of research

We analyze the function and dysfunction of ion channels in the thalamocortical system by performing electrophysiological recordings in single neurons, neuronal networks and behaving animals.
Techniques: Patch-clamp recordings; field potential recordings; single unit recordings; EEG; Ca2+ imaging; voltage-sensitive dye imaging; PCR; Western blot; immunohistochemistry; computer modeling using NEURON.
Ion channels of interest: BKCa; CaV1-2; HCN1-4; K2P; KCa3; Kir; KV1, KV4, KV7; NaV.

ChemBIon project description:

Hyperpolarization-activated cyclic nucleotide-gated cation (HCN) channels, the molecular correlate of the hyperpolarization-activated inward current (Ih), have important functions in controlling neuronal excitability and generating rhythmic oscillatory activity. The four subtypes (HCN1-4) all differ in their biophysical and modulatory properties and show differential distribution in the brain. Direct sensitivity to cAMP originating from a C-terminal cyclic nucleotide binding domain (CNBD) is the central modulatory motif of HCN channels. More recently the tetratricopeptide repeat-containing Rab8b-interacting protein (TRIP8b), a brain-specific accessory subunit which binds to the C-terminus, was found to be important for HCN channel regulation. This project aims to achieve a better understanding of the cell type-specific function of HCN channel isoforms and their interaction with auxiliary subunits under normal and inflammatory conditions. In addition, the restorative potential of HCN channel activators under conditions of reduced channel function will be assessed in brain slices. As a necessary bridge for the future use of channel modulators in disease models, their effects in the living brain will be proven by electrophysiological recordings in vivo combined with intrathalamic substance application.

  • Curriculum vitae

    1981 - 1982

    Civil engineering, Ruhr-Universität Bochum

    1982 - 1990

    Diploma, Biology, Ruhr-Universität Bochum

    1990 - 1993

    Ph.D., Natural sciences, Ruhr-Universität Bochum

    1993 - 1995

    Department for Biological Sciences, University of Iowa, USA, Postdoctoral research associate

    1995 - 1997

    Department for Physiology, Otto-von-Guericke-Universität Magdeburg, Helmholtz - fellow of the BMBF

    1997 - 2005

    Department for Physiology, Otto-von-Guericke-Universität Magdeburg, Scientific staff

    2005 - present

    Institute for Physiology I, Westfälische Wilhelms-Universität Münster, Scientific staff


    Habilitation and venia legendi in Physiology, Otto-von-Guericke-Universität Magdeburg


    Nomination as non-ordinary (Apl.) professor by the dean of the Medical Faculty of the Westfälische Wilhelms-Universität Münster