PUBLICATIONS

  • 2022

    • Kasper K, Abreu IN, Feussner K, Zienkiewicz K, Herrfurth C, Ischebeck T, Janz D, Majcherczyk A, Schmitt K, Valerius O, Braus GH, Feussner I, Polle A. Multi-omics analysis of xylem sap uncovers dynamic modulation of poplar defenses by ammonium and nitrate. Plant J. 2022 May 10, https://doi.org/10.1111/tpj.15802

    • Guzha A, McGee R, Scholz P, Hartken D, Lüdke D, Bauer K, Wenig M, Zienkiewicz K, Herrfurth C, Feussner I, Vlot AC, Wiermer M, Haughn G, Ischebeck T. Cell wall-localized BETA-XYLOSIDASE4 contributes to immunity of Arabidopsis against Botrytis cinerea. Plant Physiol. 2022 Apr 18, https://doi.org/10.1093/plphys/kiac165

    • Krawczyk, H.E., Sun, S., Doner, N.M., Yan, Q., Lim, M.S.S., Scholz, P., Niemeyer, P.W., Schmitt, K., Valerius, O., Pleskot, R., Hillmer, S., Braus, G.H., Wiermer, M., Mullen, R.T., Ischebeck, T., (2022) SEED LIPID DROPLET PROTEIN1, SEED LIPID DROPLET PROTEIN2 and LIPID DROPLET PLASMA MEMBRANE ADAPTOR mediate lipid droplet-plasma membrane tethering. Plant Cell, https://doi.org/10.1093/plcell/koac095

    • Scholz, P., Pejchar, P., Fernkorn, M., Škrabálková, E., Pleskot, R., Blersch, K., Munnik, T., Potocký, M. and Ischebeck, T. (2022), DIACYLGLYCEROL KINASE 5 regulates polar tip growth of tobacco pollen tubes. New Phytologist. Accepted Author Manuscript; https://doi.org/10.1111/nph.17930
    • Hertel, R., Schöne, K., Mittelstädt, C., Meißner, J., Zschoche, N., Collignon, M., Kohler, C., Friedrich, I., Schneider, D., Hoppert, M., Kuhn, R., Schwedt, I., Scholz, P., Poehlein, A., Martienssen, M., Ischebeck, T., Daniel, R., Commichau, F.M. (2022) Characterization of glyphosate-resistant Burkholderia anthina and Burkholderia cenocepacia isolates from a commercial Roundup® solution. Environnebtal Microbiology Reports, Online ahead of print; https://doi.org/10.1111/1758-2229.13022
    • Krawczyk HE, Rotsch AH, Herrfurth C, Scholz P, Shomroni O, Salinas-Riester G, Feussner I, Ischebeck T. Heat stress leads to rapid lipid remodelling and transcriptional adaptations in Nicotiana tabacum pollen tubes. Plant Physiol. 2022 Mar 18:kiac127; https:// doi.org/10.1093/plphys/kiac127

    • Krüger L, Herzberg C, Wicke D, Scholz P, Schmitt K, Turdiev A, Lee VT, Ischebeck T, Stülke J. Sustained Control of Pyruvate Carboxylase by the Essential Second Messenger Cyclic di-AMP in Bacillus subtilis. mBio. 2022 Feb 8;13(1):e0360221; https://doi.org/10.1128/mbio.03602-21

  • 2021

    • Berghoff, S.A., Spieth, L., Sun, T., Hosang, L., Depp, C., Sasmita, A.O., Vasileva, M.H., Scholz, P., Zhao, Y., Krueger-Burg, D., Wichert, S., Brown, E.R., Michail, K., Nave, K.A., Bonn, S., Odoardi, F., Rossner, M., Ischebeck, T., Edgar, J.M., Saher, G. (2021) Neuronal cholesterol synthesis is essential for repair of chronically demyelinated lesions in mice. Cell Rep. 2021 Oct 26;37(4):109889; https://doi.org/10.1016/j.celrep.2021.109889
    • Pyc, M., Gidda, S.K., Seay, D., Esnay, N., Kretzschmar, F.K., Cai, Y., Doner, N.M., Greer, M.S., Hull, J.J., Coulon, D., Bréhélin, C., Yurchenko, O., de Vries, J., Valerius, O., Braus, G.H., Ischebeck, T., Chapman, K.D., Dyer, J.M., Mullen, R.T. (2021) LDIP Cooperates with SEIPIN and LDAP to Facilitate Lipid Droplet Biogenesis in Arabidopsis. Plant Cell, 33, 3076-3103; https://doi.org/10.1093/plcell/koab179
    • de Vries, S., Fürst-Jansen, J.M.R., Irisarri, I., Dhabalia Ashok, A., Ischebeck T., Feussner, K., Abreu, I.N., Petersen, M., Feussner, I., de Vries, J. (2021) The evolution of the phenylpropanoid pathway entailed pronounced radiations and divergences of enzyme families. Plant Journal, 107, 975-1002; https://doi.org/10.1101/2021.05.27.445924
    • Isolation of Lipid Droplets for Protein and Lipid Analysis. Horn, P.J., Chapman, K.D., Ischebeck T. (2021) Methods in Molecular Biology, 2295, 295-320; https://doi.org/10.1007/978-1-0716-1362-7_16
    • Berghoff, S.A., Spieth, L., Sun, T., Hosang, L., Schlaphoff, L., Depp, C., Düking, T., Winchenbach, J., Neuber, J., Ewers, D., Scholz, P., van der Meer, F., Cantuti-Castelvetri, L., Sasmita, A.O., Meschkat, M., Ruhwedel, T., Möbius, W., Sankowski, R., Prinz, M., Huitinga, I., Sereda, M.W., Odoardi, F., Ischebeck, T., Simons, M., Stadelmann-Nessler, C., Edgar, J.M., Nave, K.-A. and Saher, G. (2021) Microglia facilitate repair of demyelinated lesions via post-squalene sterol synthesis. Nature Neuroscience, 24, 47-60; https://doi.org/10.1038/s41593-020-00757-6
    • Doner, N., Seay, D., Mehling, M., Sun, S., Gidda, S., Schmitt, K., Braus, G.H., Ischebeck, T., Chapman, K.D., Dyer, J.M., Mullen, R.T. (2021) Arabidopsis thaliana EARLY RESPONSIVE TO DEHYDRATION 7 Localizes to Lipid Droplets via its Senescence Domain. Frontiers in Plant Science, 12, 658961; https://doi.org/10.3389/fpls.2021.658961
    • Krüger, L., Herzberg, C., Rath, H., Pedreira, T., Ischebeck, T., Poehlein, A., Gundlach, J., Daniel, R.,Völker, U., Mäder, U., Stülke, J (2021) Essentiality of c-di-AMP in Bacillus subtilis: Bypassing mutations converge in potassium and glutamate homeostasis. PLOS Genetics, 7, e1009092; https://doi.org/10.1371/journal.pgen.1009092
    • Goemann, J., Herrfurth, C., Zienkiewicz, A., Ischebeck, T., Haslam, T., Hornung, E., Feussner, I. (2021) Sphingolipid long-chain base hydroxylation influences plant growth and callose deposition in Physcomitrium patens. New Phytologist, 231, 297-314; https://doi.org/10.1111/nph.17345
    • Vollheyde K., Hornung, E., Herrfurth, C., Ischebeck, T., Feussner, I. (2021) Plastidial wax ester biosynthesis as a tool to synthesize shorter and more saturated wax esters. Biotechnol Biofuels, 14, 238; https://doi.org/10.1186/s13068-021-02062-1

     

  • 2020

    • Kretzschmar, F.K., Doner, N., Krawczyk, H.E., Scholz, P., Schmitt, K., Valerius, O., Braus, G., Mullen, R.T., Ischebeck, T. (2020) Identification of low-abundant lipid droplet proteins in seeds and seedlings. Plant Physiology, 182, 1326-1345; https://doi.org/10.1104/pp.19.01255
    • Ischebeck, T.#, Krawczyk, H.E., Mullen, R.T., Dyer, J.M., Chapman, K.D. (2020) Lipid droplets in plants and algae: Distribution, formation, turnover and function. Seminars in Cell & Developmental Biology, epub ahead of print; https://doi.org/10.1016/j.semcdb.2020.02.014
    • De Vries, J. # and Ischebeck, T. # (2020) Ties between stress and lipid droplets predate seeds. Trends in Plant Science, 25:1203-1214; https://doi.org/10.1016/j.tplants.2020.07.017
    • Greer, M.S., Cai, Y., Gidda, S.K., Esnay, N., Kretzschmar, F.K., Seay, D., McClinchie, E., Ischebeck, T., Mullen, R.T., Dyer, J.M., Chapman, K.D. (2020) SEIPIN Isoforms Interact with the Membrane-tethering Protein VAP27-1 for Lipid Droplet Formation. The Plant Cell; https://doi.org/10.1105/tpc.19.00771
    • Stenzel, I., Ischebeck, T., Vu-Becker, L. H., Riechmann, M., Krishnamoorthy, P., Fratini, M., eilmann, I. (2020). Coordinated localization and antagonistic function of NtPLC3 and PI4P 5-kinases in the subapical plasma membrane of tobacco pollen tubes. Plants, 9, E452; https://doi.org/10.3390/plants9040452
    • Scholz, P.; Anstatt, J.; Krawczyk, H.E.; Ischebeck, T. (2020) Signalling Pinpointed to the Tip: The Complex Regulatory Network That Allows Pollen Tube Growth. Plants, 9, 1098; https://doi.org/10.3390/plants9091098

     

     

     

  • 2019

    • Alevra, M., Mandad, S., Ischebeck, T., Urlaub, H., Rizzoli, S.O., Fornasiero, E.F. (2019) A mass spectrometry workflow for measuring protein turnover rates in vivo. Nature Protocols, 14, 3333–3365; https://doi.org/10.1038/s41596-019-0222-y
    • Touraine, B., Vignols, F., Przybyla-Toscano, J., Ischebeck, T., Dhalleine, T., Wu, H.C., Magno, C., Berger, N., Couturier, J., Dubos, C., Feussner, I., Caffarri, S., Havaux, M., Rouhier, N. and Gaymard, F. (2019) The iron-sulfur protein NFU2 plays a predominant role in branched-chain amino acid synthesis in Arabidopsis roots. Journal of Experimental Botany, 70, 1875-1889; https:doi.org/10.1093/jxb/erz050
    • Djian, B., Hornung, E., Ischebeck, T. and Feussner, I. (2019) The green microalga Lobosphaera incisa harbors an arachidonate 15S‐lipoxygenase. Plant Biology, 21, 131-142; https://doi.org/10.1111/plb.12920
    • Dormeyer, M., Lentes, S., Richts, B., Heermann, R., Ischebeck, T., and Commichau, F. M. (2019) Variants of the Bacillus subtilis LysR-type regulator GltC with altered activator and repressor function. Frontiers in Microbiology, 10; https://doi.org/10.3389/fmicb.2019.02321
    • Wicke, D., Schulz, L.M., Lentes, S., Scholz, P., Poehlein, A., Gibhardt, J., Daniel, R., Ischebeck, T. and Commichau, F.M. (2019) Identification of the first glyphosate transporter by genomic adaptation. Environmental Microbiology, 21,1287-1305; https://doi.org/10.1111/1462-2920.14534

     

     

     

  • 2014-2018

    2018

    • Kretzschmar, F.K., Mengel, L.F., Müller, A., Schmitt, K., Blersch, K.F., Valerius, O., Braus, G., and Ischebeck, T. (2018) PUX10 is a lipid droplet-localized scaffold protein that interacts with CDC48 and is involved in the degradation of lipid droplet proteins. The Plant Cell. 30, 2137-2160; https://doi.org/10.1105/tpc.18.00276
    • Müller, A.O. and Ischebeck, T. (2018) Characterization of the enzymatic activity and physiological function of the lipid droplet-associated triacylglycerol lipase AtOBL1. New Phytologist, 217, 1062-1076; https://doi.org/10.1111/nph.14902
    • Hafidh, S.S., Potesil, D., Muller, K., Fila, J., Michailidis, C., Herrmannova, A., Fecikova, J., Ischebeck, T., Valasek, L.S., Zdrahal, Z., and Honys, D. (2018) Dynamics of Nicotiana tabacum pollen sequestrome defined by subcellular coupled omics. Plant Physiology 178, 258-282; https://doi.org/10.1104/pp.18.00648
    • Mandad, S., Rahman, R.U., Centeno, T.P., Vidal, R.O., Wildhagen, H., Rammner, B., Keihani, S., Opazo, F., Urban, I., Ischebeck, T., Kirli, K., Benito, E., Fischer, A., Yousefi, R.Y., Dennerlein, S., Rehling, P., Feussner, I., Urlaub, H., Bonn, S., Rizzoli, S.O. and Fornasiero, E.F. (2018) The codon sequences predict protein lifetimes and other parameters of the protein life cycle in the mouse brain. Sci Rep, 8, 16913; https://doi.org/10.1038/s41598-018-35277-8
    • Fornasiero, E.F., Mandad, S., Wildhagen, H., Alevra, M., Rammner, B., Keihani, S., Opazo, F., Urban, I., Ischebeck, T., Sakib, M.S., Fard, M.K., Kirli, K., Centeno, T.P., Vidal, R.O., Rahman, R.-U., Benito, E., Fischer, A., Dennerlein, S., Rehling, P., Feussner, I., Bonn, S., Simons, M., Urlaub, H. and Rizzoli, S.O. (2018) Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions. Nature Communications, 9, 4230; https://doi.org/10.1038/s41467-018-06519-0

     

    2017

    • Pyc, M., Cai, Y., Gidda, S.K., Yurchenko, O., Park, S., Kretzschmar, F.K., Ischebeck, T., Valerius, O., Braus, G.H., Chapman, K.D., Dyer, J.M. and Mullen, R.T. (2017) Arabidopsis lipid drop-associated protein (LDAP) - interacting protein (LDIP) influences lipid droplet size and neutral lipid homeostasis in both leaves and seeds. The Plant Journal, 92, 1182-1201; https://doi.org/10.1111/tpj.13754
    • Rotsch, A.H., Kopka, J., Feussner, I. and Ischebeck, T. (2017) Central metabolite and sterol profiling divides tobacco male gametophyte development and pollen tube growth into eight metabolic phases. The Plant Journal, 92, 129-146; https://doi.org/10.1111/tpj.13633
    • Siegler, H., Valerius, O., Ischebeck, T., Popko, J., Tourasse, N.J., Vallon, O., Khozin-Goldberg, I., Braus, G.H. and Feussner, I. (2017) Analysis of the lipid body proteome of the oleaginous alga Lobosphaera incisa. BMC Plant Biology, 17, 98; https://doi.org/10.1186/s12870-017-1042-2
    • Rosenberg, J., Ischebeck, T., and Commichau, F. M. (2017) Vitamin B6 metabolism in microbes and approaches for fermentative production. Biotechnol Advances 35, 31-40; https://doi.org/10.1016/j.biotechadv.2016.11.004
    • Reuss, D. R., Altenbuchner, J., Mader, U., Rath, H., Ischebeck, T., Sappa, P. K., Thurmer, A., Guerin, C., Nicolas, P., Steil, L., Zhu, B., Feussner, I., Klumpp, S., Daniel, R., Commichau, F. M., Volker, U., and Stulke, J. (2017) Large-scale reduction of the Bacillus subtilis genome: Consequences for the transcriptional network, resource allocation, and metabolism. Genome Research 27, 289-299; https://doi.org/10.1101/gr.215293.116
    • Müller, A. O., Blersch, K. F., Gippert, A. L., and Ischebeck, T. (2017) Tobacco pollen tubes - a fast and easy tool to study lipid droplet association of plant proteins. The Plant Journal 89, 1055-1064; https://doi.org/10.1111/tpj.13441

     

    2016

    • Popko, J., Herrfurth, C., Feussner, K., Ischebeck, T., Iven, T., Haslam, R., Hamilton, M., Sayanova, O., Napier, J., Khozin-Goldberg, I., and Ivo Feussner (2016) Metabolome analysis reveals betaine lipids as major source for triglyceride formation, and the accumulation of sedoheptulose during nitrogen-starvation of Phaeodactylum tricornutum. PloS one 11, e0164673; https://doi.org/10.1371/journal.pone.0164673
    • Ischebeck, T. (2016) Lipids in pollen — They are different. BBA - Molecular and Cell Biology of Lipids 1861, 1315-1328; https://doi.org/10.1016/j.bbalip.2016.03.023
    • Hofvander, P., Ischebeck, T., Turesson, H., Kushwaha, S. K., Feussner, I., Carlsson, A. S., and Andersson, M. (2016) Potato tuber expression of Arabidopsis WRINKLED1 increase triacylglycerol and membrane lipids while affecting central carbohydrate metabolism. Plant Biotechnology Journal 14, 1883-1898; https://doi.org/10.1111/pbi.12550
    • Heilmann, I., and Ischebeck, T. (2016) Male functions and malfunctions: the impact of phosphoinositides on pollen development and pollen tube growth. Plant Reproduction, 1-18; https://doi.org/10.1007/s00497-015-0270-6
    • Großhennig, S., Ischebeck, T., Gibhardt, J., Busse, J., Feussner, I., and Stülke, J. (2016) Hydrogen sulfide is a novel potential virulence factor of Mycoplasma pneumoniae: characterization of the unusual cysteine desulfurase/desulfhydrase HapE. Molecular Microbiology 100, 42-54; https://doi.org/10.1111/mmi.13300
    • Eng, F., Haroth, S., Feussner, K., Meldau, D., Rekhter, D., Ischebeck, T., Brodhun, F., and Feussner, I. (2016) Optimized jasmonic acid production by Laslodiplodia theobromae in submerged fermentation reveals formation of valuable plant specialized metabolites. PLoS ONE 11, e0167627; https://doi.org/10.1371/journal.pone.0167627

     

    2015

    • Steinhorst, L., Mähs, A., Ischebeck, T., Zhang, C., Zhang, X., Arendt, S., Schultke, S., Heilmann, I., and Kudla, J. (2015) Vacuolar CBL-CIPK12 Ca(2+)-sensor-kinase complexes are required for polarized pollen tube growth. Current Biology 25, 1475-1482; https://doi.org/10.1016/j.cub.2015.03.053
    • Stannek, L., Thiele, M. J., Ischebeck, T., Gunka, K., Hammer, E., Volker, U., and Commichau, F. M. (2015) Evidence for synergistic control of glutamate biosynthesis by glutamate dehydrogenases and glutamate in Bacillus subtilis. Environmental Microbiology 17, 3379-3390; https://doi.org/10.1111/1462-2920.12813

     

    2014

    • Ischebeck, T.#, Valledor, L., Lyon, D., Gingl, S., Nagler, M., Meijon, M., Egelhofer, V., and Weckwerth, W.# (2014) Comprehensive cell-specific protein analysis in early and late pollen development from diploid microsporocytes to pollen tube growth. Molecular & Cellular Proteomics 13, 295-310; https://doi.org/10.1074/mcp.M113.028100
    • Bellaire, A.*, Ischebeck, T.*, Staedler, Y.*, Weinhaeuser, I., Mair, A., Parameswaran, S., Ito, T., Schönenberger, J., and Weckwerth, W. (2014) Metabolism and development – integration of micro computed tomography data and metabolite profiling reveals metabolic reprogramming from floral initiation to silique development. New Phytologist 202, 322-335; https://doi.org/10.1111/nph.12631

     

     

     

     

  • 2006-2013

    • Ischebeck, T.*, Werner, S.*, Krishnamoorthy, P., Lerche, J., Meijon, M., Stenzel, I., Löfke, C., Wiessner, T., Im, Y. J., Perera, I. Y., Iven, T., Feussner, I., Busch, W., Boss, W. F., Teichmann, T., Hause, B., Persson, S., and Heilmann, I. (2013) Phosphatidylinositol 4,5-bisphosphate influences PIN polarization by controlling clathrin-mediated membrane trafficking in Arabidopsis. The Plant Cell 25, 4894-4911; https://doi.org/10.1105/tpc.113.116582
    • Chaturvedi, P., Ischebeck, T., Egelhofer, V., Lichtscheidl, I., and Weckwerth, W. (2013) Cell-specific analysis of the tomato pollen proteome from pollen mother cell to mature pollen provides evidence for developmental priming. Journal of Proteome Research 12, 4892-4903; https://doi.org/10.1021/pr400197p
    • Stenzel, I., Ischebeck, T., Quint, M., and Heilmann, I. (2012) Variable regions of PI4P 5-kinases direct PtdIns(4,5)P2 towards alternative regulatory functions in tobacco pollen tubes. Frontiers in Plant Science 2; https://doi.org/10.3389/fpls.2011.00114
    • Mähs, A.*, Ischebeck, T.*, Heilig, Y.*, Stenzel, I., Hempel, F., Seiler, S., and Heilmann, I. (2012) The essential phosphoinositide kinase MSS-4 is required for polar hyphal morphogenesis, localizing to sites of growth and cell fusion in Neurospora crassa. PLoS ONE 7, e51454; https://doi.org/10.1371/journal.pone.0051454
    • Ischebeck, T., Stenzel, I., Hempel, F., Jin, X., Mosblech, A., and Heilmann, I. (2011) Phosphatidylinositol-4,5-bisphosphate influences Nt-Rac5-mediated cell expansion in pollen tubes of Nicotiana tabacum. The Plant Journal 65, 453-468; https://doi.org/10.1111/j.1365-313X.2010.04435.x
    • Ischebeck, T.*, Vu, L. H.*, Jin, X., Stenzel, I., Löfke, C., and Heilmann, I. (2010) Functional cooperativity of enzymes of phosphoinositide conversion according to synergistic effects on pectin secretion in tobacco pollen tubes. Molecular Plant 3, 870-881; https://doi.org/10.1093/mp/ssq031
    • Ischebeck, T., Seiler, S., and Heilmann, I. (2010) At the poles across kingdoms: phosphoinositides and polar tip growth. Protoplasma 240, 13-31; https://doi.org/10.1007/s00709-009-0093-0
    • Stenzel, I., Ischebeck, T., König, S., Holubowska, A., Sporysz, M., Hause, B., and Heilmann, I. (2008) The type B phosphatidylinositol-4-phosphate 5-kinase 3 is essential for root hair formation in Arabidopsis thaliana. The Plant Cell 20, 124-141; https://doi.org/10.1105/tpc.107.052852
    • Löfke, C., Ischebeck, T., König, S., Freitag, S., and Heilmann, I. (2008) Alternative metabolic fates of phosphatidylinositol produced by phosphatidylinositol synthase isoforms in Arabidopsis thaliana. Biochemical Journal 413, 115-124; https://doi.org/10.1042/BJ20071371
    • König, S., Ischebeck, T., Lerche, J., Stenzel, I., and Heilmann, I. (2008) Salt-stress-induced association of phosphatidylinositol 4,5-bisphosphate with clathrin-coated vesicles in plants. Biochemical Journal 415, 387-399; https://doi.org/10.1042/BJ20081306
    • Ischebeck, T., Stenzel, I., and Heilmann, I. (2008) Type B phosphatidylinositol-4-phosphate 5-kinases mediate Arabidopsis and Nicotiana tabacum pollen tube growth by regulating apical pectin secretion. The Plant Cell 20, 3312-3330; https://doi.org/10.1105/tpc.108.059568
    • Pidkowich, M. S., Nguyen, H. T., Heilmann, I., Ischebeck, T., and Shanklin, J. (2007) Modulating seed b-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil. PNAS 104, 4742-4747; https://doi.org/10.1073/pnas.0611141104
    • Ischebeck, T., Zbierzak, A. M., Kanwischer, M., and Dormann, P. (2006) A salvage pathway for phytol metabolism in Arabidopsis. Journal of Biological Chemistry 281, 2470-2477; https://doi.org/10.1074/jbc.M509222200