Optical spectroscopy under the microscope: Method development and applications in colloid science

Prof. Andreas Zumbusch (Universität Konstanz)

Optical microscopy has always relied on spectroscopic techniques for contrast generation. The most straightforward approach is to combine it with linear techniques such as absorption and fluorescence spectroscopy. Over the last three decades, especially fluorescence excitation in the form of confocal fluorescence microscopy has become an important method present in all cell biology laboratories. However, fluorescence microscopy also is a valuable tool in material science. This will be exemplified by investigations of the structural dynamics of colloidal systems close to the glass transition. Yet, the great success of spectroscopic approaches in microscopy is not restricted to linear methods. Meanwhile, non-linear spectroscopy has found ample applications in modern optical microscopy with the aim of overcoming limitations encountered with linear approaches.

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