Institut für Infektiologie	Tel. (0251) 83-5 64 66 Fax: (0251) 83-5 64 67 e-mail: ifi@uni-muenster.de www: http://zmbe.uni-muenster.de/
Von-Esmarch-Str. 56 48149 Münster Direktor: Prof. Dr. rer. nat. M. Alexander Schmidt
Forschungsschwerpunkte 2003 - 2004    zurück    weiter

Neue Strategien zur Entwicklung einer Meningitis Vakzine   Bakterielle Meningitiden sind für eine hohe Morbidität und Mortalität in den frühen Lebensjahren (mit)verantwortlich. In diesem Forschungsprojekt untersuchen wir Möglichkeiten, aus pathogenen N. meningitidis und E. coli isolierte und mit geeigneten T Zell Epitopen konjugierte Kohlenhydrate zur transdermalen Immunisierung einzusetzen. Ausgewählte Publikationen: Ide, T., Michgehl, S., Knappstein, S., Heusipp, G., and Schmidt, M. A. (2003) Differential modulation by Ca²+ of type III secretion of diffuse-adhering enteropathogenic Escherichia coli (DA-EPEC). Infect. Immun., 71, 1725-1732. Brückener, K. E., el Bayâ, A., Galla, H.-J., and Schmidt, M.A. (2003). Permeabilization of cerebral endothelial barriers by pertussis toxin involves the PKC effector pathway and does not depend on elevated levels of cAMP. J. Cell Sci. 116, 1837-1846. Lügering, A., Benz, I., Knochenhauer, S., Ruffing, M., and Schmidt, M.A. (2003) The Pix pilus adhesin of the uropathogenic Escherichia coli strain X2194 (O2:K-:H6) is related to Pap pili but exhibits a truncated regulatory region. Microbiology, 149(6), 1387-1397. Laarmann, S., and Schmidt, M. A. (2003) Identification of an integral membrane glyco-protein as receptor for the Escherichia coli AIDA autotransporter adhesin. Microbiology, 149, 1871-1882. Janka, A., Bielaszewska, M., Dobridt, U., Greune, L., Schmidt, M. A. and Karch, H. (2003) The cytolethal distending toxin (cdt) gene cluster in enterohemorrhagic Escherichia coli O157:H- and O157:H7: characterization and evolutionary considerations. Infect. Immun., 71, 3634-3638. Meckelein, B., Externest D., Schmidt, M. A., and Frey, A. (2003) Contribution of serum immunoglobulin transudate to the antibody immune status of intestinal secretions: influence of different sampling procedures. Clin. Diagn. Lab. Inv., 10, 831-834. Heusipp, G., Schmidt, M. A., and Miller, V. L. (2003) Identification of rpoE and nadB as host responsive elements of Yersinia enterocolitica. FEMS Microbiol. Lett. 226, 291-298. Benz, I., Tapadar, D., Buddenborg, C., Voß, T., and Schmidt, M. A. (2003) The AIDA system – The bacterial AIDA autotransporter as a presentation module for the development of live oral vaccines. BIOforum Internatl. 7(6), 335-337. Olivier, V., Meisen, I., Meckelein, B., Hirst. T. R., Peter-Katalinic, J., Schmidt, M. A., and Frey A. (2003) Influence of targeting ligand flexibility on receptor binding of particulate drug delivery ystems. Bioconjugate Chem., 14, 1203-1208. Schmidt, M. A., Riley, L. W., and Benz, I. (2003) Sweet new world – glycoproteins in bacterial pathogens. Trends Microbiol., 11(12), 554-561. Bielaszewska, M., Fell, M., Greune, L., Prager, R., Fruth, A., Tschäpe, H., Schmidt, M. A., and Karch, H. (2004) Characterization of the cytolethal distending toxin genes and expression in Shiga toxin-producing Escherichia coli strains of non-O157 serogroups. Infect. Immun. 72,1812-1816. Heusipp, G., Nelson, K. M., Schmidt, M. A. , and Miller, V. L. (2004) Regulation of htrA expression in Yersinia enterocolitica. FEMS Microbiology Letters, 231, 227-235. Lügering, A., Floer, M., Lügering, N., Cichon, C., Schmidt, M. A., Domschke, W., and Kucharzik, T. (2004) Characterization of M cell formation and associated mononuclear cells during indomethacin-induced intestinal inflammation. Clin. Exp. Immunol., 136, 232-238. Knappstein, S., Ide, T., Schmidt, M. A., and Heusipp, G. (2004) A novel role for α1-antitrypsin in host defense against infection with atypical enteropathogenic Escherichia coli. Infect. Immun. 72(8), 4344-4350. Gärtner, J. F., and Schmidt, M. A. (2004) LEE pathogenicity islands of atypical enteropathogenic Escherichia coli (ATEC): Comparative Analysis and Transfer of Function. Infect. Immun. 72 (11), 6722-6728. Westendorf, A. M., Gunzer, F., Schmidt, M. A., Buer, J., and Bruder, D. (2005) Intestinal immunity of E. coli NISSLE 1917: a safe carrier for therapeutic molecules. FEMS Immunology & Medical Microbiology, 43, 373-384. Büning, J. Schmitz, M., Repenning, B., Ludwig D., Kaiserlian, D., Schmidt, M. A., Strobel, S., and Zimmer, K.-P. (2005) Interferon-gamma mediates antigen trafficking to MHC class II positive late endosomes of enterocytes. Eur. J. Immunol., 35,831-842. Mogensen, J. E., Tapadar, D., Schmidt, M. A., and Otzen, D. E. (2005) Biophysical Characterisation of the Transmembrane Domain of the Escherichia coli Autotransporter AIDA. Extreme Stability of the β-Domain. Biochemistry, 44, 4533-4545. Lügering, A., Floer, M., Westphal, S., Maaser, C., Spahn, T. W., Schmidt, M. A., Domschke, W., and Kucharzik, T. (2005) Absence of CCR6 inhibits CD4+ regulatory T cell development and M cell formation inside Peyer's patches. Am. J. Pathol. , 166(6), 1647-1654. Müller, D., Benz, I., Tapadar, D., Buddenborg, C., Greune, L., and Schmidt, M. A. (2005) Homologous interactions of the AIDA autotransporter on the bacterial surface preferably result in dimer formation. Infect. Immun., 73(7), 3851-3859. Fälker, S., Schmidt, M. A., and Heusipp, G. (2005) DNA methylation in Yersinia enterocolitica: Role of the DNA adenine methyltransferase in mismatch repair and regulation of virulence factors. Microbiology, 151, 2291-2299.