RESEARCH - Nicolas Cornelissen

© IBC

 

Starting my independent research, I am interested in combining organic chemistry with enzymes to obtain nucleoside-5′-triphosphates (NTPs). These NTPs can serve as cofactors in enzymatic reactions to either elucidate biological function or to generate nucleic acids with improved properties for medical applications.

© IBC

 

 

 

 

 

 

 

 

 

If you are interested in a research internship please contact me!

 

  • CV Nicolas Cornelissen

    since 2022 Senior scientist, Institute of Biochemistry, University of Münster
    2022 Dr. rer. nat., University of Münster
    2015-2018 MSc Chemistry, University of Münster
    2011-2015 BSc Chemistry, University of Münster
    2010-2011 Civil service
    2010 Abitur, Meerbusch Gymnasium

  • Publikationen

    ORCID: 0000-0002-3625-7612

    18. Mitton-Fry, R. M.; Rasche, R.; Lawrence-Dörner, A.-M.; Eschenbach, J.; Tekath, A.; Rentmeister, A.; Kümmel, D.; Cornelissen, N. V., Structure-guided engineering of a polyphosphate kinase 2 class III from an Erysipelotrichaceae bacterium to produce base-modified purine nucleotides. RSC Chem. Biol. 2025. DOI: 10.1039/D5CB00108K

    17. Cornelissen, N.V.*, Hoffmann, A.*, Ghosh, P., Pignot, Y.L., Erguven, M., and Rentmeister, A. Chimeric cofactors enable methyltransferase-catalyzed prenylation. Chem 2025 (online ahead of print). DOI: 10.1016/j.chempr.2025.102538

    16. Schöning, J., Tekath, A., Cornelissen, N.V., Hoffmann, A., and Rentmeister, A. SAM-III aptamer enables detection of enzymatic SAM analogue generation. Chem. Commun. 2024, 61, 370-373. DOI: 10.1039/d4cc04860a.

    15. Mitton-Fry, R.M., Eschenbach, J.,  Schepers, H., Rasche, R., Erguven, M., Kümmel, D., Rentmeister, A., and Cornelissen, N.V.
    Chemo-enzymatic production of base-modified ATP analogues for polyadenylation of RNA.
    Chem. Sci. 2024, 15, 13068-13073. DOI:10.1039/D4SC03769C

    14. Hoffmann, A.*, Schülke, K.H.*, Hammer, S.C., Rentmeister, A., Cornelissen, N.V. Comparative S-adenosyl-l-methionine analogue generation for selective biocatalytic Friedel-Crafts alkylation. Chem. Commun. 2023,59, 5463-5466. DOI: 10.1039/D3CC01036H

    13. Cornelissen, N.V.*, Mineikaitė, R.*, Erguven, M., Muthmann, N., Peters, A., Bartels, A., and Rentmeister, A.
    Post-synthetic benzylation of the mRNA 5′ cap via enzymatic cascade reactions.
    Chem. Sci. 2023, 14, 10962-10970. DOI: https://doi.org/10.1039/D3SC03822J

    12. Cornelissen, N.V., Rentmeister, A.
    Ribozyme for stabilized SAM analogue modifies RNA in cells.
    Nat. Chem. 2023, 15, 1486–1487. DOI: https://doi.org/10.1038/s41557-023-01354-3

    11. Hartstock, K., Kueck, N.A., Spacek, P., Ovcharenko, A., Hüwel, S., Cornelissen, N.V., Bollu, A. Dieterich, C., Rentmeister, A.
    MePMe-seq: antibody-free simultaneous m6A and m5C mapping in mRNA by metabolic propargyl labeling and sequencing.
    Nat. Commun. 2023, 14, 7154. DOI: https://doi.org/10.1038/s41467-023-42832-z

    10. Mohr, M. K. F., Saleem-Batcha, R., Cornelissen, N. V., Andexer, J. N.
    Enzymatic Synthesis of l-Methionine Analogues and Application in a Methyltransferase Catalysed Alkylation Cascade.
    Chem. Eur. J. 2023, 29, e202301503. DOI: https://doi.org/10.1002/chem.202301503

    9. Gericke, L., Mhaindarkar, D., Karst, L. C., Jahn, S., Kuge, M., Mohr, M. K. F., Gagsteiger, J., Cornelissen, N. V., Wen, X., Mordhorst, S., Jessen, H. J., Rentmeister, A., Seebeck, F. P., Layer, G., Loenarz, C., Andexer, J. N.
    Biomimetic S-Adenosylmethionine Regeneration Starting from Multiple Byproducts Enables Biocatalytic Alkylation with Radical SAM Enzymes.
    ChemBioChem 2023, 24, e202300133. DOI: https://doi.org/10.1002/cbic.202300133

    8. Cornelissen, N. V.; Hoffmann, A.; Rentmeister, A., DNA-Methyltransferasen und AdoMet-Analoga als Werkzeuge für die Molekularbiologie und Biotechnologie. Chemie Ingenieur Technik 2023, 95 (4), 518-530. DOI: 10.1002/cite.202200174

    7. Ergüven, M.; Cornelissen, N. V.; Peters, A.; Karaca, E.; Rentmeister, A., Enzymatic generation of double modified AdoMet analogues and their application in cascade reactions with different methyltransferases. Chembiochem 2022, 23, e202200511. DOI: 10.1002/cbic.202200511

    6. Peters, A.; Herrmann, E.; Cornelissen, N. V.; Klöcker, N.; Kümmel, D.; Rentmeister, A.
    Visible-Light Removable Photocaging Groups Accepted by MjMAT Variant: Structural Basis and Compatibility with DNA and RNA Methyltransferases.
    ChemBioChem 2022, 23 (1), e202100437. DOI: 10.1002/cbic.202100437

    5. Kueck, N. A.; Ovcharenko, A.; Hartstock, K.; Cornelissen, N. V.; Rentmeister, A., Chemoenzymatic labeling of RNA to enrich, detect and identify methyltransferase-target sites. Methods Enzymol. 2021, 658, 161-190. DOI: 10.1016/bs.mie.2021.06.006

    4. Rickhoff, J.*; Cornelissen, N. V.*; Beuse, T.; Rentmeister, A.; Jan Ravoo, B.
    Multiresponsive hydrogels and organogels based on photocaged cysteine.
    Chem. Commun. 2021, 57 (48), 5913-5916. DOI: https://doi.org/10.1039/D1CC01363G

    3. Michailidou, F.*; Klöcker, N.*; Cornelissen, N. V.*; Singh, R. K.; Peters, A.; Ovcharenko, A.; Kümmel, D.; Rentmeister, A.
    Engineered SAM Synthetases for Enzymatic Generation of AdoMet Analogs with Photocaging Groups and Reversible DNA Modification in Cascade Reactions.
    Angew. Chem. Int. Ed. 2021, 60 (1), 480-485. DOI: https://doi.org/10.1002/anie.202012623

    2. Cornelissen, N. V.; Michailidou, F.; Muttach, F.; Rau, K.; Rentmeister, A.
    Nucleoside-modified AdoMet analogues for differential methyltransferase targeting.
    Chem. Commun. 2020, 56 (14), 2115-2118. DOI: https://doi.org/10.1039/C9CC07807J

    1. Hartstock, K.; Nilges, B. S.; Ovcharenko, A.; Cornelissen, N. V.; Püllen, N.; Lawrence-Dörner, A. M.; Leidel, S. A.; Rentmeister, A.
    Enzymatic or In Vivo Installation of Propargyl Groups in Combination with Click Chemistry for the Enrichment and Detection of Methyltransferase Target Sites in RNA.
    Angew. Chem. Int. Ed. 2018, 57 (21), 6342-6346. DOI: 10.1002/anie.201800188


    *The authors contributed equally.