RESEARCH - Nicolas Cornelissen



Starting my independent research, I am interested in combining organic chemistry with enzymes to obtain nucleoside-5′-triphosphates (NTPs). These NTPs can serve as cofactors in enzymatic reactions to either elucidate biological function or to generate nucleic acids with improved properties for medical applications.











If you are interested in a research internship please contact me!


  • CV Nicolas Cornelissen

    since 2022 Senior scientist, Institute of Biochemistry, University of Münster
    2022 Dr. rer. nat., University of Münster
    2015-2018 MSc Chemistry, University of Münster
    2011-2015 BSc Chemistry, University of Münster
    2010-2011 Civil service
    2010 Abitur, Meerbusch Gymnasium
  • Publikationen

    ORCID: 0000-0002-3625-7612

    Hartstock, K., Kueck, N.A., Spacek, P., Ovcharenko, A., Hüwel, S., Cornelissen, N.V., Bollu, A. Dieterich, C., Rentmeister, A.
    MePMe-seq: antibody-free simultaneous m6A and m5C mapping in mRNA by metabolic propargyl labeling and sequencing.
    Nat. Commun. 2023, 14, 7154. DOI:

    Cornelissen, N.V.*, Mineikaitė, R.*, Erguven, M., Muthmann, N., Peters, A., Bartels, A., and Rentmeister, A.
    Post-synthetic benzylation of the mRNA 5′ cap via enzymatic cascade reactions.
    Chem. Sci. 2023, 14, 10962-10970. DOI:

    Hoffmann, A.*, Schülke, K.H.*, Hammer, S.C., Rentmeister, A., Cornelissen, N.V. Comparative S-adenosyl-l-methionine analogue generation for selective biocatalytic Friedel-Crafts alkylation.
    Chem. Commun. 2023, 59, 5463-5466. DOI:

    Cornelissen, N.V., Rentmeister, A.
    Ribozyme for stabilized SAM analogue modifies RNA in cells.
    Nat. Chem. 2023, 15, 1486–1487. DOI:

    Mohr, M. K. F., Saleem-Batcha, R., Cornelissen, N. V., Andexer, J. N.
    Enzymatic Synthesis of l-Methionine Analogues and Application in a Methyltransferase Catalysed Alkylation Cascade.
    Chem. Eur. J. 2023, 29, e202301503. DOI:

    Gericke, L., Mhaindarkar, D., Karst, L. C., Jahn, S., Kuge, M., Mohr, M. K. F., Gagsteiger, J., Cornelissen, N. V., Wen, X., Mordhorst, S., Jessen, H. J., Rentmeister, A., Seebeck, F. P., Layer, G., Loenarz, C., Andexer, J. N.
    Biomimetic S-Adenosylmethionine Regeneration Starting from Multiple Byproducts Enables Biocatalytic Alkylation with Radical SAM Enzymes.
    ChemBioChem 2023, 24, e202300133. DOI:

    Peters, A.; Herrmann, E.; Cornelissen, N. V.; Klöcker, N.; Kümmel, D.; Rentmeister, A.
    Visible-Light Removable Photocaging Groups Accepted by MjMAT Variant: Structural Basis and Compatibility with DNA and RNA Methyltransferases.
    ChemBioChem 2022, 23 (1), e202100437. DOI:

    Rickhoff, J.*; Cornelissen, N. V.*; Beuse, T.; Rentmeister, A.; Jan Ravoo, B.
    Multiresponsive hydrogels and organogels based on photocaged cysteine.
    Chem. Commun. 2021, 57 (48), 5913-5916. DOI:

    Michailidou, F.*; Klöcker, N.*; Cornelissen, N. V.*; Singh, R. K.; Peters, A.; Ovcharenko, A.; Kümmel, D.; Rentmeister, A.
    Engineered SAM Synthetases for Enzymatic Generation of AdoMet Analogs with Photocaging Groups and Reversible DNA Modification in Cascade Reactions.
    Angew. Chem. Int. Ed. 2021, 60 (1), 480-485. DOI:

    Cornelissen, N. V.; Michailidou, F.; Muttach, F.; Rau, K.; Rentmeister, A.
    Nucleoside-modified AdoMet analogues for differential methyltransferase targeting.
    Chem. Commun. 2020, 56 (14), 2115-2118. DOI:

    Hartstock, K.; Nilges, B. S.; Ovcharenko, A.; Cornelissen, N. V.; Püllen, N.; Lawrence-Dörner, A. M.; Leidel, S. A.; Rentmeister, A.
    Enzymatic or In Vivo Installation of Propargyl Groups in Combination with Click Chemistry for the Enrichment and Detection of Methyltransferase Target Sites in RNA.
    Angew. Chem. Int. Ed. 2018, 57 (21), 6342-6346. DOI: 10.1002/anie.201800188

    *The authors contributed equally.