Dr. Michael Sperling (Dipl. Chem.)
© Michael Sperling
Dr. Michael Sperling (Dipl. Chem.)
European Virtual Institute for Speciation Analysis (EVISA)
Room 118
Mendelstraße 11
48149 Münster
Germany
Tel: +49 (251) 83-36489
Fax: +49 (251) 980-2681
michael.sperling@uni-muenster.de

Personal

Chief Managing Director of the European Virtual Institute for Speciation Analysis (EVISA) at the University of Muenster

Research interests

Development of robust methods for speciation analysis

Development of binary speciation techniques for species screening

Hyphenated techniques using atomic spectrometric detection

Automation of analytical methods by the use of flow techniques

Development of plasma sources for atomic and mass spectrometry

Bioimaging by mass spectrometry

Imaging by µXRF (instrumental and synchrotron-based)

ResearcherID: C-2584-2008

Publications

  • Fingerhut S., Niehoff A., Sperling M., Jeibmann A., Paulus W., Niederstadt T., Allkemper T., Heindel W., Holling M., Karst U. . ‘Spatially resolved quantification of gadolinium deposited in the brain of a patient treated with gadolinium-based contrast agents.’ Journal of Trace Elements in Medicine and Biology 45, No. null: 125-130. doi: 10.1016/j.jtemb.2017.10.004.
  • Clases D., Sperling M., Karst U. . ‘Analysis of metal-based contrast agents in medicine and the environment.’ TrAC - Trends in Analytical Chemistry null, No. null. doi: 10.1016/j.trac.2017.12.011. [In Press]
  • Thyssen G., Keil C., Wolff M., Sperling M., Kadow D., Haase H., Karst U. . ‘Bioimaging of the elemental distribution in cocoa beans by means of LA-ICP-TQMS.’ Journal of Analytical Atomic Spectrometry 33, No. 2: 187-194. doi: 10.1039/c7ja00354d.

  • Thyssen GM, Holtkamp M, Kaulfürst-Soboll H, Wehe CA, Sperling M, von Schaewen A, Karst U. . ‘Elemental bioimaging by means of LA-ICP-OES: investigation of the calcium, sodium and potassium distribution in tobacco plant stems and leaf petioles.’ Metallomics 9, No. 6: 676-684. doi: 10.1039/c7mt00003k.
  • Wiemann M., Vennemann A., Blaske F., Sperling M., Karst U. . ‘Silver nanoparticles in the lung: Toxic effects and focal accumulation of silver in remote organs.’ Nanomaterials 7, No. 12. doi: 10.3390/nano7120441.
  • Aschner M., Palinski C., Sperling M., Karst U., Schwerdtle T., Bornhorst J. . ‘Imaging metals in: Caenorhabditis elegans.’ Metallomics 9, No. 4: 357-364. doi: 10.1039/c6mt00265j.
  • Clases D., Birka M., Sperling M., Faust A., Karst U. . ‘Isobaric dilution analysis as a calibration tool for long lived radionuclides in ICP-MS.’ Journal of Trace Elements in Medicine and Biology 40, No. null: 97-103. doi: 10.1016/j.jtemb.2017.01.002.
  • de Vega R., Sanchez M., Eiro N., Vizoso F., Sperling M., Karst U., Medel A. . ‘Multimodal laser ablation/desorption imaging analysis of Zn and MMP-11 in breast tissues.’ Analytical and Bioanalytical Chemistry null, No. null: 1-10. doi: 10.1007/s00216-017-0537-x. [In Press]
  • Hachmöller O., Aichler M., Schwamborn K., Lutz L., Werner M., Sperling M., Walch A., Karst U. . ‘Investigating the influence of standard staining procedures on the copper distribution and concentration in Wilson's disease liver samples by laser ablation-inductively coupled plasma-mass spectrometry.’ Journal of Trace Elements in Medicine and Biology 44, No. null: 71-75. doi: 10.1016/j.jtemb.2017.06.002.
  • Hachmöller O., Zibert A., Zischka H., Sperling M., Groba S., Grünewald I., Wardelmann E., Schmidt H., Hartmut H. . ‘Spatial investigation of the elemental distribution in Wilson's disease liver after D-penicillamine treatment by LA-ICP-MS.’ Journal of Trace Elements in Medicine and Biology 44, No. null: 26-31. doi: 10.1016/j.jtemb.2017.05.008.

  • Niehoff AC , Schulz J , Soltwisch J , Meyer S, Kettling H , Sperling M, Jeibmann A , Dreisewerd K, Francesconi KA , Schwerdtle T , Karst U. . ‘Imaging by Elemental and Molecular Mass Spectrometry Reveals the Uptake of an Arsenolipid in the Brain of Drosophila melanogaster.’ ANALYTICAL CHEMISTRY 88, No. 10: 5258-5263. doi: 10.1021/acs.analchem.6b00333.
  • Birka, M , Wehe, CA, Hachmöller, O , Sperling M , Karst U. . ‘Tracing gadolinium-based contrast agents from surface water to drinking water by means of speciation analysis.’ JOURNAL OF CHROMATOGRAPHY A 1440: 105-111. doi: 10.1016/j.chroma.2016.02.050.
  • Niehoff AC , Wachsmuth L , Schmid F , Sperling M, Faber C , Karst U. . ‘Quantification of Manganese Enhanced Magnetic Resonance Imaging based on Spatially Resolved Elemental Mass Spectrometry.’ CHEMISTRYSELECT 1, No. 2: 264-266. doi: 10.1002/slct.201600058.
  • Hogeback J, Schwarzer M , Wehe CA , Sperling M, Karst U. . ‘Investigating the adduct formation of organic mercury species with carbonic anhydrase and hemoglobin from human red blood cell hemolysate by means of LC/ESI-TOF-MS and LC/ICP-MS.’ METALLOMICS 8, No. 1: 101-107. doi: 10.1039/c5mt00186b.
  • Hachmöller O, Aichler M, Schwamborn K , Lutz L, Werner M, Sperling M , Walch A , Karst U. . ‘Element bioimaging of liver needle biopsy specimens from patients with Wilson's disease by laser ablation-inductively coupled plasma-mass spectrometry.’ JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY 35: 97-102. doi: 10.1016/j.jtemb.2016.02.001.
  • Hachmöller O , Buzanich AG , Aichler M , Radtke M , Dietrich D , Schwamborn K , Lutz L , Werner M , Sperling M , Walch A , Karst U. . ‘Elemental bioimaging and speciation analysis for the investigation of Wilson's disease using mu XRF and XANES.’ METALLOMICS 8, No. 7: 648-653. doi: 10.1039/c6mt00001k.
  • Niehoff A., Grünebaum J., Moosmann A., Mulac D., Söbbing J., Niehaus R., Buchholz R., Kröger S., Wiehe A., Wagner S., Sperling M., von Briesen H., Langer K., Karst U. . ‘Quantitative bioimaging of platinum group elements in tumor spheroids.’ Analytica Chimica Acta 938, No. null: 106-113. doi: 10.1016/j.aca.2016.07.021.
  • Birka M., Roscher J., Holtkamp M., Sperling M., Karst U. . ‘Investigating the stability of gadolinium based contrast agents towards UV radiation.’ Water Research 91, No. null: 244-250. doi: 10.1016/j.watres.2016.01.012.
  • Birka M., Wehe C., Hachmöller O., Sperling M., Karst U. . ‘Tracing gadolinium-based contrast agents from surface water to drinking water by means of speciation analysis.’ Journal of Chromatography A 1440, No. null: 105-111. doi: 10.1016/j.chroma.2016.02.050.
  • Hogeback J., Schwarzer M., Wehe C., Sperling M., Karst U. . ‘Investigating the adduct formation of organic mercury species with carbonic anhydrase and hemoglobin from human red blood cell hemolysate by means of LC/ESI-TOF-MS and LC/ICP-MS.’ Metallomics 8, No. 1: 101-107. doi: 10.1039/c5mt00186b.
  • Birka M., Wehe C., Telgmann L., Sperling M., Karst U. . ‘ICPMS for environmental and bioimaging research.’ Spectroscopy (Santa Monica) 31, No. 1: 36-37.
  • Hachmöller O., Aichler M., Schwamborn K., Lutz L., Werner M., Sperling M., Walch A., Karst U. . ‘Element bioimaging of liver needle biopsy specimens from patients with Wilson's disease by laser ablation-inductively coupled plasma-mass spectrometry.’ Journal of Trace Elements in Medicine and Biology 35, No. null: 97-102. doi: 10.1016/j.jtemb.2016.02.001.
  • Hachmöller O., Buzanich A., Aichler M., Radtke M., Dietrich D., Schwamborn K., Lutz L., Werner M., Sperling M., Walch A., Karst U. . ‘Elemental bioimaging and speciation analysis for the investigation of Wilson's disease using μxRF and XANES.’ Metallomics 8, No. 7: 648-653. doi: 10.1039/c6mt00001k.
  • Niehoff A., Schulz J., Soltwisch J., Meyer S., Kettling H., Sperling M., Jeibmann A., Dreisewerd K., Francesconi K., Schwerdtle T., Karst U. . ‘Imaging by Elemental and Molecular Mass Spectrometry Reveals the Uptake of an Arsenolipid in the Brain of Drosophila melanogaster.’ Analytical Chemistry 88, No. 10: 5258-5263. doi: 10.1021/acs.analchem.6b00333.

  • Köppen C., Reifschneider O., Castanheira I., Sperling M., Karst U., Ciarimboli G. . ‘Quantitative imaging of platinum based on laser ablation-inductively coupled plasma-mass spectrometry to investigate toxic side effects of cisplatin.’ Metallomics 7, No. 12: 1595-1603. doi: 10.1039/c5mt00226e.
  • Niehaus R, Sperling M, Karst U. . ‘Study on aerosol characteristics and fractionation effects of organic standard materials for bioimaging by means of LA-ICP-MS.’ Journal of Analytical Atomic Spectrometry 30, No. 10: 2056-2065. doi: 10.1039/C5JA00221D.
  • Niehoff A., Bauer O., Kröger S., Fingerhut S., Schulz J., Meyer S., Sperling M., Jeibmann A., Schwerdtle T., Karst U. . ‘Quantitative Bioimaging to Investigate the Uptake of Mercury Species in Drosophila melanogaster.’ Analytical Chemistry 87, No. 20: 10392-10396. doi: 10.1021/acs.analchem.5b02500.
  • Mak C., Wehe C., Sperling M., Karst U. . ‘Identification and quantification of electrochemically generated metabolites of thyroxine by means of liquid chromatography/electrospray-mass spectrometry and countergradient liquid chromatography/inductively coupled plasma-mass spectrometry.’ Journal of Chromatography A 1419, No. null: 81-88. doi: 10.1016/j.chroma.2015.09.076.
  • Wehe C., Thyssen G., Herdering C., Raj I., Ciarimboli G., Sperling M., Karst U. . ‘Elemental Bioimaging by Means of Fast Scanning Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry.’ Journal of the American Society for Mass Spectrometry 26, No. 8: 1274-1282. doi: 10.1007/s13361-015-1141-y.
  • Birka M., Wentker K., Lusmöller E., Arheilger B., Wehe C., Sperling M., Stadler R., Karst U. . ‘Diagnosis of Nephrogenic Systemic Fibrosis by means of Elemental Bioimaging and Speciation Analysis.’ Analytical Chemistry 87, No. 6: 3321-3328. doi: 10.1021/ac504488k.
  • Karst U., Sperling M. . ‘2015 European Winter Conference on Plasma Spectrochemistry.’ Journal of Analytical Atomic Spectrometry 30, No. 10: 2035-2037. doi: 10.1039/c5ja90044a.

  • Wehe CA, Pieper I, Holtkamp M, Thyssen GM, Sperling M, Schwerdtle T, Karst U. . ‘On-line species-unspecific isotope dilution analysis in the picomolar range reveals the time- and species-depending mercury uptake in human astrocytes.Analytical and bioanalytical chemistry 406, No. 7: 1909-16. doi: 10.1007/s00216-013-7608-4.
  • Nowak S, Gesell M, Holtkamp M, Sperling M, Karst U, Buscher W. . ‘Low Gas Flow Inductively Coupled Plasma Optical Emission Spectrometry for the Analysis of Food Samples after Microwave Digestion.’ Talanta 129: 575-578. doi: 10.1016/j.talanta.2014.06.045.
  • Wehe C., Niehoff A., Thyssen G., Sperling M., Karst U. . ‘Rapid cell mode switching and dual laser ablation inductively coupled plasma mass spectrometry for elemental bioimaging.’ Rapid Communications in Mass Spectrometry 28, No. 23: 2627-2635. doi: 10.1002/rcm.7054.
  • Reifschneider O., Schutz C.L., Brochhausen C., Hampel G., Ross T., Sperling M., Karst U. . ‘Quantitative bioimaging of p-boronophenylalanine in thin liver tissue sections as a tool for treatment planning in boron neutron capture therapy.’ Analytical and Bioanalytical Chemistry null, No. null. doi: 10.1007/s00216-014-8012-4. [In Press]
  • Blaske F., Reifschneider O., Gosheger G., Wehe C.A., Sperling M., Karst U., Hauschild G., Holl S. . ‘Elemental bioimaging of nanosilver-coated prostheses using X-ray fluorescence spectroscopy and laser ablation-inductively coupled plasma-mass spectrometry.’ Analytical Chemistry 86, No. 1: 615-620. doi: 10.1021/ac4028577.
  • Wehe C.A., Beyer G., Sperling M., Ciarimboli G., Karst U. . ‘Assessing the intracellular concentration of platinum in medulloblastoma cell lines after Cisplatin incubation.’ Journal of Trace Elements in Medicine and Biology 28, No. 2: 166-172. doi: 10.1016/j.jtemb.2014.01.001.
  • Niehoff A.-C., Moosmann A., Sobbing J., Wiehe A., Mulac D., Wehe C.A., Reifschneider O., Blaske F., Wagner S., Sperling M., Von Briesen H., Langer K., Karst U. . ‘A palladium label to monitor nanoparticle-assisted drug delivery of a photosensitizer into tumor spheroids by elemental bioimaging.’ Metallomics 6, No. 1: 77-81. doi: 10.1039/c3mt00223c.

  • Birka M, Wehe CA, Telgmann L, Sperling M, Karst U. . ‘Sensitive quantification of gadolinium-based magnetic resonance imaging contrast agents in surface waters using hydrophilic interaction liquid chromatography and inductively coupled plasma sector field mass spectrometry.Journal of chromatography. A 1308: 125-31. doi: 10.1016/j.chroma.2013.08.017.
  • Telgmann L, Sperling M, Karst U. . ‘Determination of gadolinium-based MRI contrast agents in biological and environmental samples: a review.Analytica chimica acta 764: 1-16. doi: 10.1016/j.aca.2012.12.007.
  • Sperling M., Karst U. . ‘Metallomics: An emerging interdisciplinary science.’ Analytical and Bioanalytical Chemistry 405, No. 6: 1289-1290. doi: 10.1007/s00216-012-6619-x.
  • Brauckmann C., Faber H., Lanvers-Kaminsky C., Sperling M., Karst U. . ‘Influence of cimetidine and its metabolites on Cisplatin-Investigation of adduct formation by means of electrochemistry/liquid chromatography/electrospray mass spectrometry.’ Journal of Chromatography A 1279, No. null: 49-57. doi: 10.1016/j.chroma.2012.12.069.
  • Reifschneider O., Wehe C.A., Diebold K., Becker C., Sperling M., Karst U. . ‘Elemental bioimaging of haematoxylin and eosin-stained tissues by laser ablation ICP-MS.’ Journal of Analytical Atomic Spectrometry 28, No. 7: 989-993. doi: 10.1039/c3ja50046b.
  • Blaske F., Stork L., Sperling M., Karst U. . ‘Adduct formation of ionic and nanoparticular silver with amino acids and glutathione.’ Journal of Nanoparticle Research 15, No. 9. doi: 10.1007/s11051-013-1928-3.
  • Reifschneider O., Wehe C.A., Raj I., Ehmcke J., Ciarimboli G., Sperling M., Karst U. . ‘Quantitative bioimaging of platinum in polymer embedded mouse organs using laser ablation ICP-MS.’ Metallomics 5, No. 10: 1440-1447. doi: 10.1039/c3mt00147d.
  • Herdering C., Wehe C.A., Reifschneider O., Raj I., Ciarimboli G., Diebold K., Becker C., Sperling M., Karst U. . ‘Laser ablation based bioimaging with simultaneous elemental and molecular mass spectrometry: Towards spatially resolved speciation analysis.’ Rapid Communications in Mass Spectrometry 27, No. 23: 2588-2594. doi: 10.1002/rcm.6726.
  • Herdering C., Reifschneider O., Wehe C.A., Sperling M., Karst U. . ‘Ambient molecular imaging by laser ablation atmospheric pressure chemical ionization mass spectrometry.’ Rapid Communications in Mass Spectrometry 27, No. 23: 2595-2600. doi: 10.1002/rcm.6727.
  • Brauckmann C, Wehe CA, Kieshauer M, Lanvers-Kaminsky C, Sperling M, Karst U. . ‘The interaction of platinum-based drugs with native biologically relevant proteins.Anal Bioanal Chem 405, No. 6: 1855-64. doi: 10.1007/s00216-012-6410-z.

  • Meermann B, Sperling M. . ‘Hyphenated techniques as tools for speciation analysis of metal-based pharmaceuticals: developments and applications.Analytical and bioanalytical chemistry 403, No. 6: 1501-1522. doi: 10.1007/s00216-012-5915-9.
  • Holtkamp M, Wehe CA, Blaske F, Holtschulte C, Sperling M, Karst U. . ‘Nitrogen purged TXRF for the quantification of silver and palladium.’ JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 27: 1799-1802. doi: 10.1039/c2ja30117b.
  • Pfeifer T, Janzen R, Steingrobe T, Sperling M, Franze B, Engelhard C, Buscher W. . ‘Development of a novel low-flow ion source/sampling cone geometry for inductively coupled plasma mass spectrometry and application in hyphenated techniques.’ Spectrochimica Acta Part B 76: 48-55. doi: 10.1016/j.sab.2012.06.053.
  • Telgmann L, Wehe CA, Birka M, Kunnemeyer J, Nowak S, Sperling M, Karst U. . ‘Speciation and isotope dilution analysis of gadolinium-based contrast agents in wastewater.’ Environmental Science and Technology 46, No. 21: 11929-11936. doi: 10.1021/es301981z.

  • Wehe CA, Bornhorst J, Holtkamp M, Sperling M, Galla H-J, Schwerdtle T, Karst U. . ‘Fast and low sample consuming quantification of manganese in cell nutrient solutions by flow injection ICP-QMS.’ Metallomics 3, No. 12: 1291-1296. doi: 10.1039/c1mt00170a.
  • Janzen R, Schwarzer M, Sperling M, Vogel M, Schwerdtle T, Karst U. . ‘Adduct formation of Thimerosal with human and rat hemoglobin: a study using liquid chromatography coupled to electrospray time-of-flight mass spectrometry (LC/ESI-TOF-MS).Metallomics : integrated biometal science 3, No. 8: 847-52. doi: 10.1039/c1mt00043h.
  • Telgmann L, Holtkamp M, Künnemeyer J, Gelhard C, Hartmann M, Klose A, Sperling M, Karst U. . ‘Simple and rapid quantification of gadolinium in urine and blood plasma samples by means of total reflection X-ray fluorescence (TXRF).’ Metallomics 30: 1766-1773.
  • Sperling M., Szpunar J. . ‘Editorial: Speciation issue.’ Journal of Analytical Atomic Spectrometry 26, No. 1: 22. doi: 10.1039/c0ja90031a.

  • Bomke S, Sperling M, Karst U. . ‘Organometallic derivatizing agents in bioanalysis.Analytical and bioanalytical chemistry 397, No. 8: 3483-94. doi: 10.1007/s00216-010-3611-1.
  • Ciarimboli G, Deuster D, Knief A, Sperling M, Holtkamp M, Edemir B, Pavenstädt H, Lanvers-Kaminsky C, am Zehnhoff-Dinnesen A, Schinkel AH, Koepsell H, Jürgens H, Schlatter E. . ‘Organic cation transporter 2 mediates cisplatin-induced oto- and nephrotoxicity and is a target for protective interventions.Am J Pathol 176, No. 3: 1169-80. doi: 10.2353/ajpath.2010.090610.
  • Wilken A, Janzen R, Holtkamp M, Nowak S, Sperling M, Vogel M, Karst U. . ‘Investigation of the interaction of Mercurochrome® constituents with proteins using liquid chromatography/mass spectrometry.’ Analytical and Bioanalytical Chemistry 397, No. 8: 3525-3532. doi: 10.1007/s00216-010-3842-1.

  • Trumpler S, Lohmann W, Meermann B, Buscher W, Sperling M, Karst U. . ‘Interaction of thimerosal with proteins-ethylmercury adduct formation of human serum albumin and beta-lactoglobulin A.’ METALLOMICS 1, No. 1: 87-91.
  • Nowak S, Trümpler S, Meermann B, Wiesmüller GA, Buscher W, Sperling M, Karst U.Investigations of mercury interaction with human blood components by means of Static High Sensitivity ICP (SHIP).’ contributed to the TraceSpec, Mainz, Deutschland, .
  • Trumpler S, Nowak S, Meermann B, Wiesmuller GA, Buscher W, Sperling M, Karst U. . ‘Detoxification of mercury species-an in vitro study with antidotes in human whole blood.’ Analytical and Bioanalytical Chemistry 395, No. 6: 1929-1935. doi: 10.1007/s00216-009-3105-1.

  • Scheffer A, Engelhard C, Sperling M, Buscher W. . ‘ICP-MS as a new tool for the determination of gold nanoparticles in bioanalytical applications.Analytical and Bioanalytical Chemistry 390, No. 1: 249-52. doi: 10.1007/s00216-007-1576-5.

  • Scheffer A, Engelhard C, Sperling M, Buscher W. . ‘Introducing wet aerosols into the static high sensitivity ICP (SHIP).Analytical and Bioanalytical Chemistry 388, No. 8: 1605-13. doi: 10.1007/s00216-007-1378-9.

  • Francesconi K.A., Sperling M. . ‘Speciation analysis with HPLC-mass spectrometry: Time to take stock.’ Analyst 130, No. 7: 998-1001. doi: 10.1039/b504485p.
  • Proust N., Buscher W., Sperling M. . ‘Speciation and the Emerging Legislation.’ In Handbook of Elemental Speciation II - Species in the Environment, Food, Medicine and Occupational Health, edited by Cornesis R, Caruso J, Crews H, Heumann K, 737-744. John Wiley & Sons, Ltd. doi: 10.1002/0470856009.ch4.
  • Buscher W., Sperling M. . ‘Introduction.’ In Handbook of Elemental Speciation II - Species in the Environment, Food, Medicine and Occupational Health, edited by Cornelis Rita, Caruso Joe, Crews Helen, Heumann Klaus, 1-4. John Wiley & Sons, Ltd. doi: 10.1002/0470856009.ch1.

  • Cornelis R., Buscher W., Sperling M., Donard O.F.X., Proust N., Rosenberg E., Milacic R. . ‘The EC networking on elemental speciation revisited.’ Accreditation and Quality Assurance 9, No. 4-5: 268-271. doi: 10.1007/s00769-004-0761-x.