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WWU-iGEM Team 2022


The iGEM team Münster is one of the winners of the Impact Grant 2022. Out of 250 submissions of the worldwide iGEM teams we are one of the 90 winners of the Impact Grant. This reflects the importance of our project and the grant bring us closer to the Grand Jamboree.

Manuskript published: "Insights in the Complex DegU, DegS, and Spo0A Regulation System of Paenibacillus polymyxa by CRISPR-Cas9-Based Targeted Point Mutations"

19th of May 2022 

Today, the article by Meliawati et al. was published in the Journal Applied and Environmental Microbiology.
In this manuscript, we have again further developed our existing CRISPR-Cas tool for Paenibacillus polymyxa. With this further development, it is now possible to introduce targeted point mutations into specific target genes. Using these point mutations, we have for the first time investigated the complex regulation by DegS, DegU and Spo0A by analyzing the mutants for swarming behavior, sporulation and exopolysaccharide production as well as expression of hyfrolyzing enzymes. This is a first step towards a better understanding of the overall regulation in Paenibacillus polymyxa. Congratulations to Meliawati Meliawati for the outstanding work done.

16nd of December 2021

Manuscript published: "CRISPR-Cas9-mediated Large Cluster Deletion and Multiplex Genome Editing in Paenibacillus polymyxa"

Today the article by Meliawati et al. was published in ACS synthetic Biology.  In this manuscript, we have further developed our existing CRISPR-Cas tool for P. polymyxa. With this further development, entire gene clusters can now be deleted using only one gRNA, and we were able to demonstrate for the first time a systematic approach in the selection criteria required for the localization of the gRNA. Furthermore, this system now allows multiple gene knock-outs, gene replacements, as well as multiple deletions of whole gene clusters and the simultaneous replacement of gene clusters with reporter genes with an impressive efficiency. This opens up completely new possibilities for genome editing in P. polymyxa. Congratulations to Meliawati Meliawati for the outstanding work done.

2nd of December 2021

Manuscript published: "Structural elucidation of the fucose containing polysaccharides of Paenibacillus polymyxa DSM 365."

Today the article by Schilling et al. was published in Carbohydrate Polymers. In this manuscript, we identified the chemical structure of one of the polysaccharides of P. polymyxa using CRISPR-Cas9 based gene knock-outs. Here we show how well synthetic biology can be combined with structural analysis of polysaccharides, and how in vivo biopolymer engineering can be used in different ways. Congratulations to Christoph Schilling for the great work done.

17th of September 2021

Joint working group on synthetic biology

Synthetic biology encompasses various disciplines in the natural and life sciences and includes the design of biological networks, systems or even entire cells that do not occur in nature.

In January 2020, the DECHEMA and the Society for Biochemistry and Molecular Biology (GBM) agreed to merge the activities of the Division for Systems Biology and Synthetic biology and the GBM study group Synthetic biology in the Joint Synthetic Biology Working Group. In February 2021, the German Chemical Society (GDCh) joined the agreement; The German Botanical Society (DBG) was added in June 2021.

On September 17th, during the German Conference on Synthetic Biology 2021, the general assembly elected the steering committee 2021-24.

2nd of September 2021

Paper accepted: "Exploiting unconventional prokaryotic hosts for industrial biotechnology"

Today the opinion article by Prof. Schmid and colleagues was accepted in Trends in Biotechnology. In this manuscript the potential of unconventional host is depicted and compared to established model prokaryotic organims. One of these is Paenibacillus polymyxa, by others the main work horses of the AG Schmid with a very high potential for biotechnological applications. In this opinion article main factors such as multi-factorial traits like tolerance, growth efficiency, or handling which cannot always be engineered into existing model organisms are highlighted. We hope you enjoy the article and are looking forward to the feedback of the scientific community.