Localize via spectral counts

Description

Localize proteins or peptides according to their spectral counts in measurements coming from two different isolations, eg. chloroplast vs. non-chloroplast. Items are localized by spectral count ratio if possible, if no ratio can be determined because there are no identifications in isolation B, the localization is determined via the spectral counts in isolation A.

Input files

• at least 1 A isolation PSM list file (.csv)
• at least 1 B isolation PSM list file (.csv)

Output files

• Localization results (localization-results.csv)

Context

Parameters

Scope

Choices: Protein level (default), Peptide level

Minimal A/B ratio

Default: 5.0

Minimal A scan count

Default: 5

Source code

localize-by-spectral-counts.rb, localize-by-spectral-counts.yaml (GitHub)