Rapid mechanical deformationof cells
2PP-DLW is used to enable a microfluidic approach in which cells are mechanically deformed as they pass through a constriction smaller than the cell diameter. The compression and shear forces result in the formation of transient holes that enable the diffusion of material from the surrounding buffer into the cell. This method is employed with the aim of delivering macromolecules of interest to almost any cell type at high throughput. We are currently working on optimizing the constriction of the microfluidic chip design, considering both delivery efficiency and cell viability. The microfluidic chips were fabricated using 2PP-DLW patterning of organic-ceramic polymer compounds and consists of cell-scattering and deformation zones, where each zone contains an array of constrictions with adapted parameters. The chip was designed by arranging areas devices side by side. Experiments are performed to achieve a better understanding of the mechanisms of membrane disruption. The method will be used to deliver materials such as proteins and RNA to a variety of cell types including embryonic stem cells and immune cells.