Juan Pablo Fuenzalida University of Münster Department of Plant Biology and Biotechnology Nanobiotechnology Group Schlossgarten 3 48149 Münster
Email: Net:	jpfuenzalidaw@gmail.com www.uni-muenster.de/Biologie.IBBP/aggoycoolea/index.html

Biopolymer-based soft nanoparticles: fundamentals of self-assembling and surface modification to confer them with targeting capacity and strategies to trace their fate in mammalian cells in vitro

Fuenzalida/Goycoolea investigate polysaccharide-protein and polysaccharide-polysaccharide interactions from a physical chemistry viewpoint and how this understanding can be exploited to design advanced drug delivery vehicles. He discovered that alginate molecular weight and, more so, block composition influence its affinity for both chitosan and lysozyme in nanocomplex systems. To this end, he has used diverse biophysical techniques, including dynamic light scattering, zeta potential analysis, ITC, fluorescence spectroscopy titration, and synchrotron SAXS (col. Swamy). In parallel, he is addressing the more fundamental question of what is the fate of chitosan-based nanoparticles after interacting with a model cell epithelium (col. Moerschbacher). To this end, chitosan affinity proteins fused to fluorescent proteins are being used to label the surface of the nanoparticles and then by FRET and CSLM to colocalize the nanoparticles after incubation with the cells.

Last update 17.04.2013

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