Imperial College of Science, London, United Kingdom

Group Description

Brief description of legal entity
Imperial College is one of the world leading academic institutions. The Neuropathology Unit is integral part of the UK Multiple Sclerosis and Parkinson’s Disease Society Tissue Banks at the Division of Brain Sciences. Based at the Hammersmith Campus, it has lab facilities including cell culture, DNA, RNA and protein analysis and tissue analysis. As part of the UK MS&PDSTB, we have considerable experience in neurodegenerative and neuroinflammatory conditions. The Groups have long experience in the field and are in a unique position to conduct this project; there are ongoing cooperative studies on the role of microglia in neuroinflammation and share studies with other institutions such as the Wolfson Molecular Imaging Centre in Manchester that is also part of this application.

Main tasks
We established techniques to analyse the inflammatory response in human tissues as well as in vitro models using the microglial cell line BV2. Knock-down models using siRNA are also available to analyse single molecules in the inflammatory response. We have recently started the optimisation of protocols for extraction and analysis of human microglia from post-mortem injured brains and laser-based microdissection techniques to analyse microglia DNA and RNA from tissue sections.
Studies using animal models will be developed by the end of 2013 in collaboration with the Wolfson Molecular Imaging Centre in Manchester.

Participation in INMiND:  WP7 / WP8 / WP12.

Previous experience relevant to INMiND tasks

F. Roncaroli has several papers on neuroinflammation and neurodegeneration; he holds an UK-MRC funded grant (see below) and he is co-principal investigator in: i) UK MS&PDS Tissue Banks at Imperial (£2,527.160 plus £467,500 via the MRC Tissue Bank Network) and ii) Assessment of the presence and extent of vascular pathology in PD (£164,680).

Imperial College London
INMiND Office
European Institute for Molecular Imaging (EIMI) Waldeyerstr. 15
· 48149 Münster
Tel: +49 251 83 49300 · Fax: +49 251 83 49313