Beteiligte Wissenschaftler: Dr. A. Rompel, Prof. Dr. Bernt Krebs

Isolierung, Charakterisierung, Kristallisation und Röntgenstrukturanalyse von tierischen und pflanzlichen violetten sauren Phosphatasen, Catecholoxidasen, Tyrosinasen und Metallothioneinen.
Spektroskopische und kinetische Untersuchungen zu den Reaktionsmechanismen.
Molekularbiologische Arbeiten zur Erzeugung von Punktmutanten für funktionelle und mechanistische Untersuchungen.

Veröffentlichungen

• N. Sträter, B. Jasper, M. Scholte, B. Krebs, A. P. Duff, D. B. Langley, R. Han, B. A. Averill, H. C. Freeman, J. M. Guss
  "Crystal Structures of Recombinant Human Purple Acid Phosphatase in the Active Form and with an Inhibitory Conformation of the Repression Loop"
  J. Mol. Biol. 2005, 351, 233.

• J. I. Naseri, N. T. Truong, J. Hörentrup, P. Kuballa, A. Vogel, A. Rompel, F. Spener, B. Krebs
  "Porcine Purple Acid Phosphatase: Heterologous Expression, Characterization and Proteolytic Analysis "
  Archives of Biochem. and Biophys. 2004, 432, 25.

• B. Jasper, N. Sträter, B. Krebs
  "Crystal Structure of the recombinant human purple acid phosphatase"
  Z. Kristallogr. (Suppl.) 2004, 21, 18

• A. Vogel, T. Böttchers, K. Markus, H. E. Meyer, B. Krebs u. F. Spener
  "Heterologous expression and characterization of recombinanat purple acid phosphatase from red kidney bean"
  Archives of Biochemistry and biophysics 2002, 401, 164

• A. Rompel, C. Gerdemann, A. Vogel u. B. Krebs
  "Enzyme structures: Active site structural and functional aspects of purple acid phosphatase and catechol oxidase"
  Buchbeitrag in : "Inorganic Chemistry Highlights" Wiley VCH-Verlag Weinheim 155, 2002

• C. Gerdemann, C. Eicken u. B. Krebs
  "The crystal structure of catechol oxidase: New insight into the function of copper type-3 proteins"
  Acc. Chem. Res. 2002, 35, 183

• C. Gerdemann, C. Eicken, H. - J. Galla u. B. Krebs
  "Comparative modelling of the latent form of a plant catechol oxidase using a molluscan hemocyanin structure"
  J. Inorg. Biochem. 2002, 89, 155

• C. Eicken, C. Gerdemann u. B. Krebs
  "Catechol Oxidase"
  in: Handbook of Metalloproteins, John Wiley & Sons, Ltd. 2001, Chichester, Vol. 2, 1319

• A. Vogel, F. Spener u. B. Krebs
  "Purple Acid Phosphatase"
  in: Handbook of Metalloproteins, John Wiley & Sons, Ltd. 2001, Chichester, Vol. 2, 752

• C. Gerdemann, C. Eicken, A. Magrini, H. E. Meyer, A. Rompel, F. Spener u. B. Krebs
  "Isozymes of Ipomea batatas catechol oxidase differ in catalase-activity"
  Biochem. Biophys. Acta 2001, 1548, 94

• B. Krebs, C. Eicken, C. Gerdemann, K. Büldt-Karentzopoulos, A. Magrini, T. Klabunde u. J. C. Sacchettini
  "Crystal structure, sequence, and mechanism of catechol oxidase"
  J. Inorg. Biochem. 1999, 74, 35

• C. Eicken, B. Krebs u. J. C. Sacchettini
  "Catechol Oxidase - Structure and Activity"
  Curr. Opin. in Struct. Biol. 1999, 9, 677

• A. Durmus, C. Eicken, F. Spener u. B. Krebs
  "Cloning and comparative protein modeling of two purple acid phosphatase isozymes from sweet potatoes (Ipomoea batatas) "
  BBA - Protein Struct. & Mol. Enzymol. 1999, 1434, 202

• B. H. Sift, A. Durmus, W. Meyer-Klaucke u. B. Krebs
  "EXAFS Studies on the Active Site of Purple Acid Phosphatase from Sweet Potato Ipomoea batatas"
  J. Synchr. Rad. 1999, 6, 421

• A. Rompel, H. Fischer, D. Meiwes, K. Büldt-Karentzopoulos, A. Magrini, C. Eicken, C. Gerdemann u. B. Krebs
  "Substrate specificity of catechol oxidase from Lycopus europaeus and characterization of the bioproducts of enzymic caffeic acid oxidation"
  FEBS Letters 1999, 445, 103

• A. Durmus, C. Eicken, B. Sift, A. Kratel, R. Kappl, J. Hüttermann u. B. Krebs
  "The active site of purple acid phosphatase from sweet potatoes (Ipomoea batatas): Metal content and spectroscopic characterization"
  Eur. J. Biochem. 1999, 260, 709

• A. Rompel, H. Fischer, D. Meiwes, K. Büldt-Karentzopoulos, R. Dillinger, F. Tuczek, H. Witzel u. B. Krebs
  "Purification and spectroscopic studies on catechol oxidases from Lycopus europaeus and Populus nigra: Evidences for a dinuclear copper center of type 3 and spectroscopic similarities to tyrosinase and hemocyanin"
  JBIC 1999, 4, 56

• B. H. Sift, A. Durmus, W. Meyer-Klaucke u. B. Krebs
  "Photoreduction of the active sites of purple acid phosphatases by high-energy synchrotron radiation"
  EMBL Annual Report 1998, 411

• C. Eicken, F. Zippel, K. Büldt-Karentzopoulos and B. Krebs
  "Biochemical and spectroscopic characterization of catechol oxidase from sweet potatoes (Ipomoea batatas) containing a type-3 dicopper center"
  FEBS letters 1998, 486, 293

• T. Klabunde, C. Eicken, J. C. Sacchettini and B. Krebs
  "Crystal Structure of a Plant Catechol Oxidase - A Dicopper Center for Activation of Dioxygen"
  Nature Struct. Biol. 1998, 5, 1084

• H. Witzel, B. Krebs, A. Bruch, K. Büldt-Karentzopoulos, M. Dietrich, A. Durmus, C. Eicken, H. Fischer, T. Klabunde, M. Körner, D. Meiwes, D. Münstermann, R. Löcke, A. Rompel, B. Stahl, N. Sträter, H. Suerbaum
  "Phosphatase Activity and Oxygen Activation in the Active Site of the Fe(III)-Fe(II) Purple Acid Phosphatases" in: "Bioinorganic Chemistry" 1997, 385

• T. Klabunde, N. Sträter, H. Witzel u. B. Krebs
  "Violette Phosphatase: Kristallstruktur und Mechanismus"
  Z. Kristallogr. 1997, 12, 122

• T. Klabunde u. B. Krebs
  "The Dimetal Center in Purple Acid Phosphatases"
  in: " Structure and Bonding " 1997, 89, 177

• N. Sträter, W. N. Lipscomb, T. Klabunde u. B. Krebs
  "Two-Metal Ion Catalysis in Enzymatic Acyl- and Phosphoryl-Transfer Reactions"
  Angew. Chem. 1996, 108, 2158; Angew. Chem. Int. Ed. Engl. 1996, 35, 2024

• T. Klabunde, N. Sträter, R. Fröhlich, H. Witzel u. B.Krebs
  "Mechanism of Fe(III)-Zn(II) Purple Acid Phosphatase Based on Crystal Structures"
  J. Mol. Biol. 1996, 259, 737

• T. Klabunde, N. Sträter, H. Witzel u. B. Krebs
  "Purple Acid Phosphatase Containing a Dinuclear Fe(III)-Zn(II) Active Site"
  Z. Kristallogr. (Suppl.) 1995, 10, 70

• T. Klabunde, N. Sträter, H. Witzel u. B. Krebs
  "Crystal Structure of Dinuclear Fe(III)-Zn(II) Purple Acid Phosphatase"
  J. Inorg. Biochem. 1995, 59, 363

• F. Ahlers, F. Zippel, T. Klabunde, B. Krebs, R. Löcke, H. Witzel u. H.-F. Nolting
  "X-ray absorption studies of the purple acid phosphatase from red kidney beans (native enzyme, metal exchanged form)"
  Physica B 1995, 208 & 209, 731

• T. Klabunde, N. Sträter, B. Krebs u. H. Witzel
  "Structural relationship between the mammalian Fe(III)-Fe(II) and the Fe(III)-Zn(II) plant purple acid phosphatases"
  FEBS letters 1995, 367, 56

• N. Sträter, T. Klabunde, P. Tucker, H. Witzel u. B. Krebs
  "Crystal structure of dinuclear Fe(III)-Zn(II) purple acid phosphatase"
  Science 1995, 268, 1489