Analysis of new actin regulators controlling cell shape, cell dynamics and cell polarity in Drosophila hemocytes
Principal investigators: Sven Bogdan, Xiaoyi Jiang
Project time: 07/2013 - 06/2016
Project code: FF-2013-03
Drosophila blood cells (hemocytes) are an excellent model system for studying conserved gene functions in actin dynamics driven processes such as directional cell migration, phagocytosis or wound healing in a physiological environment. In order to identify new gene functions required for cell shape and cell migration we started to perform a RNAi screen in Drosophila hemocytes. High-resolution SIM and Spinning-Disc imaging techniques allows a precise phenotypic analysis of gene knockdown ex vivo and in vivo. Computational image analysis will complement this qualitative analysis and allows a classification of cellular morphologies or migration defects.
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