© AG Wedlich-Söldner

Light Microscopy

  • © AG Wedlich-Söldner

    FEI_1

    Location: Institute of Cell Dynamics and Imaging
    Group: AG Wedlich-Söldner
    Contact Person: Christian Schuberth
    Tel.: 0251 83-59053, cschuber@uni-muenster.de

    Applications & Info:

    • Epifluorescence, TIRF, circular TIRF, FRAP
    • rapid switch between modes (in ms via Galvos), hardware auto-focus, 405/491/561 nm lasers, automated c-y-z, complex protocols, climate chamber, EMCCD with up to 30 f/s
  • © AG Wedlich-Söldner

    FEI_2

    Location: Institute of Cell Dynamics and Imaging
    Group: AG Wedlich-Söldner
    Contact Person: Christian Schuberth
    Tel.: 0251 83-59053, cschuber@uni-muenster.de

    Applications & Info:

    • Epifluorescence, TIRF, circular TIRF, FRAP, laser ablation
    • rapid switch between modes (in ms via Galvos), hardware auto-focus, 405/488/pulsed 355 nm lasers, automated x-y-z, complex protocols, climate chamber, CCD camera
  • © AG Wedlich-Söldner
    CiM-funded

    FEI_3

    Location: Institute of Cell Dynamics and Imaging
    Group: AG Wedlich-Söldner
    Contact Person: Christian Schuberth
    Tel.: 0251 83-59053, cschuber@uni-muenster.de

    Applications & Info:

    • Epifluorescence, TIRF, circular TIRF, spinning disk, FRAP
    • rapid switch between modes, hardware auto-focus, 405/488/561/640 nm lasers, automated x-y-z, complex protocols, climate chamber, EMCCD with up to 30 f/s (512x512 px, 2x magnification)
  • CiM-funded

    ImageStreamX mkII

    Location: Institute of Immunology, Röntgenstraße 21
    Group: AG Roth
    Contact Person: Achmet Imam Chasa
    Tel.: 0251 83-52942, aimam@uni-muenster.de

    Applications & Info:

    • The ImageStreamX is a functional combination of a flow cytometer and a fluorescence microscope.
    • It unites the possibility of high throughput data acquisition of flow cytometry with morphological information obtained by microscopy.
    • Therefore it is possible to even analyse rare subpopulations of cells for certain expression patterns of target proteins and perform statistical data analyses.
    • Examples of possible assays: Transcription factor translocation into nucleus upon cell stimulation; Phagocytosis/endocytosis assay with analysis of subcellular localisation; Protein-protein interaction/co-localisation studies; Cell-cell binding/interactions (APC/T-Cell)
  • © EIMI

    Nikon Eclipse Ni-E & OptiGrid structured light illumination system

    Location: European Institute for Molecular Imaging, Waldeyerstr. 15
    Contact Person: Michael Kuhlmann, Tel.: 0251 83- 49312, kuhlmam@uni-muenster.de

    Applications & Info:

    • Nikon Eclipse Ni-E Microscope for brightfield and fluorescent microscopy
    • Motorized desk
    • Fully controllable by Nikon NIS Elements AR software package
    • 2 camera heads (B/W & RGB)
    • Fluorescent filter blocks for DAPI, TRITC, FITC, Cy 5 and Cy 5.5
    • automatic scanning of slides in up to 6 dimensions (X,Y,Z, Lambda (wavelength), T, multipoint) possible
    • The (optional) OptiGrid converts the illumination system of a conventional widefield microscope to a structured light illumination system and allows for the computer-assisted generation of images of nearly confocal quality.

  • CiM-funded

    Nikon Eclipse Ti with Yokogawa X1 Spinning Disc

    Location: Institute of Medical Physics and Biophysics, Robert-Koch-Str. 31
    Group: AG Galic
    Contact Person: Milos Galic
    Tel.: 0251 83-51040, galic@uni-muenster.de

    Applications & Info:

    • Confocal Spinning Disc, FRAP/PA unit
    • EMCCD camera
  • Zeiss Axio Imager M2

    Location: Institute of Neuropathology, Pottkamp 2
    Contact Person: Volker Senner
    Tel.: 0251 83-56974, senner@uni-muenster.de

    Applications & Info:

    • “Stereo Investigator” and “Neurolucida” software (MBF bioscience)
    • Brightfield, fluorescence (DAPI, GFP, Cy3) and AxioCam MRc - camera
    • 2,5x / 5x / 10x / 20x / 40x objectives and motorized stage
  • Zeiss Vert A1 Fluorescence Cell Culture Microscope

    Location: Department of Medicine A, UKM, Building D3, Room 130.082b
    Group: AG Berdel
    Contact Person: Sebastian Bäumer
    Tel.: 0251 83-44811, baumers@uni-muenster.de

    Applications & Info:

    • Inverted cell culture microscope with red/green/Dapi filters and camera soft and hardware
    • Heated table
  • Zeiss Axiovert 200M

    Location: Institute of Physiological Chemistry and Pathobiochemistry, Waldeyerstraße 15
    Group: AG Sorokin
    Contact Person: Jana Zimmermann
    Tel.: 0251 83-55585, jzimmerm@uni-muenster.de

    Applications & Info:

    • Inverse light and fluorescence microscope with incubation system and Ibidi pump system connected
    • long term live cell imaging for e.g. cell migration and adhesion assays, wound healing/scratch assays (heating/CO2 incubation system attached)
    • In combination with pump system: cell alignment under flow, shear stress/flow experiments (Ibidi pump system for long time flow and syringe pump for short time flow/shear experiments)
    • Bright field, phase contrast and fluorescence imaging combined with time lapse and/or options for motorized stage (defining several positions per time point or area scan possible)
  • Zeiss LSM 700 (I)

    Location: Institute of Physiological Chemistry and Pathobiochemistry, Waldeyerstraße 15
    Group: AG Sorokin
    Contact Person: Eva Korpos
    Tel.: 0251 83-55585, korpos@uni-muenster.de

    Applications & Info:

    • Classical confocal microscopy
    • Uses the same Zen software and the 3D datasets can be rendered with Volocity
    • Excitation lines 405nm, 488nm, 555nm and 633nm
    • Objectives: 10x/0.3, 20X/0.8, 40X/1.3DIC, 63X/1.4 Oil DIC
  • Zeiss LSM 700 (II)

    Location: Institut für Molekulare Zellbiologie, Schlossplatz 5
    Contact Person: Joanna Chiang
    Tel.: 0251 83-21761, jchia_01@uni-muenster.de

    Applications & Info:

    • Inverse fluorescence and confocal microscope with incubation system for life cell imaging
    • Equipped with Duolink (simultaneous dual channel imaging) and Hamamatsu EM-CCD ImagEM
    • Short and long-term live cell imaging for primary neurons and slice cultures (heating/CO2 incubation system)
  • Zeiss LSM510

    Location: Institute of Infectiology (ZMBE), Von-Esmarch-Str. 56
    Contact Person: Dr. Christian Rüter
    Tel.: 0251 83-56477, rueterc@uni-muenster.de

    Applications & Info:

    • confocal laser scanning microscope LSM510
  • © ZMBE

    Zeiss Axio imager Z1 equipped with sopt monochrome camera

    Location: Institute for Cell Biology
    Group: AG Raz
    Contact Person: Katsiaryna Tarbashevich
    k.tarbashevich@wwu.de, Tel. lab: 0251 83-53018, Tel. office: 83-52183

    Applications & Info:

    • Fluorescence microscopy, FRET maeasurement
    • Upright microscope capable of Multi stage imaging, Bright field imaging, Equipped with water-immersive objectives and beam splitter for FRET measurements

  • © ZMBE
    CiM co-funded

    Zeiss Lightsheet Z1 microscope

    Location: Institute for cell biology
    Group: AG Raz
    Contact Person: Łukasz Truszkowski
    l_trus01@wwu.de, Tel. lab: 0251 83-58618, Tel. office: 0251 83-52110

    Applications & Info:

    • Fluorescence imaging, lightsheet imaging
    • Equipped with water-immersive objectives. The system is suitable for high speed imaging of submerged samples