Publications

Complement receptor 3 mediates both sinking phagocytosis and phagocytic cup formation via distinct mechanisms
Walbaum S , Ambrosy B, Schütz P, Bachg AC, Horsthemke M, Leusen JHW, Mócsai A, Hanley PJ.
J Biol Chem. 2021 Jan 4;jbc.RA120.015346. doi: 10.1074/jbc.RA120.015346. Online ahead of print.
Editors Pick

Class IX Myosins: Motorized RhoGAP Signaling Molecules.
Hanley PJ, Vollmer V, Bähler M.
Adv Exp Med Biol. 2020;1239:381-389. doi: 10.1007/978-3-030-38062-5_16. PMID: 32451867

Knockout mouse models reveal the contributions of G protein subunits to complement C5a receptor-mediated chemotaxis.
van den Bos E, Ambrosy B, Horsthemke M, Walbaum S, Bachg AC, Wettschureck N, Innamorati G, Wilkie TM, Hanley PJ.
J Biol Chem. 2020 Apr 24:jbc.RA119.011984. doi: 10.1074/jbc.RA119.011984. Online ahead of print.

Time-lapse Imaging of Mouse Macrophage Chemotaxis.
van den Bos E, Walbaum S, Horsthemke M, Bachg AC, Hanley PJ.
J Vis Exp. 2020 Apr 2;(158). doi: 10.3791/60750.

A novel isoform of myosin 18A (Myo18Aγ) is an essential sarcomeric protein in mouse heart.
Horsthemke M, Nutter LMJ, Bachg AC, Skryabin BV, Honnert U, Zobel T, Bogdan S, Stoll M, Seidl MD, Mueller FU, Ravens U, Unger A, Linke WA, van Gorp PRR, de Vries AAF, Bähler M, Hanley PJ.
J Biol Chem. 2019 Feb 8. pii: jbc.RA118.004560. doi: 10.1074/jbc.RA118.004560. [Epub ahead of print] Editors Pick

Phenotypic analysis of Myo10 knockout (Myo10tm2/tm2) mice lacking full-length (motorized) but not brain-specific headless myosin X.
Bachg AC, Horsthemke M, Skryabin BV, Klasen T, Nagelmann N, Faber C, Woodham E, Machesky LM, Bachg S, Stange R, Jeong HW, Adams RH, Bähler M, Hanley PJ.
Sci Rep. 2019 Jan 24;9(1):597. doi: 10.1038/s41598-018-37160-y

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages.
Horsthemke M, Wilden J, Bachg AC, Hanley PJ.
J Vis Exp. 2018 (140), e57566.

Low density lipoprotein interferes with intracellular signaling of monocytes resulting in impaired chemotaxis and enhanced chemokinesis.
Tjaden K, Adam C, Godfrey R, Hanley PJ, Pardali E, Waltenberger J.
Int J Cardiol. 2018 Mar 15;255:160-165.

Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion.
Horsthemke M, Bachg AC, Groll K, Moyzio S, Müther B, Hemkemeyer SA, Wedlich-Söldner R, Sixt M, Tacke S, Bähler M, Hanley PJ.
J Biol Chem. 2017 Apr 28;292(17):7258-7273.

Real-time two- and three-dimensional imaging of monocyte motility and navigation on planar surfaces and in collagen matrices: roles of Rho.
Bzymek R, Horsthemke M, Isfort K, Mohr S, Tjaden K, Müller-Tidow C, Thomann M, Schwerdtle T, Bähler M, Schwab A, Hanley PJ.
Sci Rep. 2016 Apr 28;6:25016.

Mouse macrophages completely lacking Rho subfamily GTPases (RhoA, RhoB, and RhoC) have severe lamellipodial retraction defects, but robust chemotactic navigation and altered motility.
Königs V, Jennings R, Vogl T, Horsthemke M, Bachg AC, Xu Y, Grobe K, Brakebusch C, Schwab A, Bähler M, Knaus UG, Hanley PJ.
J Biol Chem. 2014 Oct 31;289(44):30772-84.

Dendritic cell motility and T cell activation requires regulation of Rho-cofilin signaling by the Rho-GTPase activating protein myosin IXb.
Xu Y, Pektor S, Balkow S, Hemkemeyer SA, Liu Z, Grobe K, Hanley PJ, Shen L, Bros M, Schmidt T, Bähler M, Grabbe S.
J Immunol. 2014 Apr 15;192(8):3559-68.

TRPC6 regulates CXCR2-mediated chemotaxis of murine neutrophils.
Lindemann O, Umlauf D, Frank S, Schimmelpfennig S, Bertrand J, Pap T, Hanley PJ, Fabian A, Dietrich A, Schwab A.
J Immunol. 2013 Jun 1;190(11):5496-505.

Roles of ion transport in control of cell motility.
Stock C, Ludwig FT, Hanley PJ, Schwab A.
Compr Physiol. 2013 Jan;3(1):59-119.

Role of ion channels and transporters in cell migration.
Schwab A, Fabian A, Hanley PJ, Stock C.
Physiol Rev. 2012 Oct;92(4):1865-913.

Transient P2X7 receptor activation triggers macrophage death independent of Toll-like receptors 2 and 4, caspase-1, and pannexin-1 proteins.
Hanley PJ, Kronlage M, Kirschning C, del Rey A, Di Virgilio F, Leipziger J, Chessell IP, Sargin S, Filippov MA, Lindemann O, Mohr S, Königs V, Schillers H, Bähler M, Schwab A.
J Biol Chem. 2012 Mar 23;287(13):10650-63.

Real-time imaging reveals that P2Y2 and P2Y12 receptor agonists are not chemoattractants and macrophage chemotaxis to complement C5a is phosphatidylinositol 3-kinase (PI3K)- and p38 mitogen-activated protein kinase (MAPK)-independent.
Isfort K, Ebert F, Bornhorst J, Sargin S, Kardakaris R, Pasparakis M, Bähler M, Schwerdtle T, Schwab A, Hanley PJ.
J Biol Chem. 2011 Dec 30;286(52):44776-87.

Cellular functions of class IX myosins in epithelia and immune cells.
Bähler M, Elfrink K, Hanley PJ, Thelen S, Xu Y.
Biochem Soc Trans. 2011 Oct;39(5):1166-8.

Autocrine purinergic receptor signaling is essential for macrophage chemotaxis.
Kronlage M, Song J, Sorokin L, Isfort K, Schwerdtle T, Leipziger J, Robaye B, Conley PB, Kim HC, Sargin S, Schön P, Schwab A, Hanley PJ.
Sci Signal. 2010 Jul 27;3(132):ra55.

Motorized RhoGAP myosin IXb (Myo9b) controls cell shape and motility.
Hanley PJ, Xu Y, Kronlage M, Grobe K, Schön P, Song J, Sorokin L, Schwab A, Bähler M.
Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12145-50.

Calcium-activated K(+) channel (K(Ca)3.1) activity during Ca(2+) store depletion and store-operated Ca(2+) entry in human macrophages.
Gao YD, Hanley PJ, Rinné S, Zuzarte M, Daut J.
Cell Calcium. 2010 Jul;48(1):19-27.

5-Hydroxydecanoate and coenzyme A are inhibitors of native sarcolemmal KATP channels in inside-out patches.
Li X, Rapedius M, Baukrowitz T, Liu GX, Srivastava DK, Daut J, Hanley PJ.
Biochim Biophys Acta. 2010 Mar;1800(3):385-91.

Ambivalent effects of diazoxide on mitochondrial ROS production at respiratory chain complexes I and III.
Dröse S, Hanley PJ, Brandt U.
Biochim Biophys Acta. 2009 Jun;1790(6):558-65.

Differential effects of volatile and intravenous anesthetics on the activity of human TASK-1.
Putzke C, Hanley PJ, Schlichthörl G, Preisig-Müller R, Rinné S, Anetseder M, Eckenhoff R, Berkowitz C, Vassiliou T, Wulf H, Eberhart L.
Am J Physiol Cell Physiol. 2007 Oct;293(4):C1319-26.

Knock-out mice reveal the contributions of P2Y and P2X receptors to nucleotide-induced Ca2+ signaling in macrophages.
del Rey A, Renigunta V, Dalpke AH, Leipziger J, Matos JE, Robaye B, Zuzarte M, Kavelaars A, Hanley PJ.
J Biol Chem. 2006 Nov 17;281(46):35147-55.

K+-independent actions of diazoxide question the role of inner membrane KATP channels in mitochondrial cytoprotective signaling.
Dröse S, Brandt U, Hanley PJ.
J Biol Chem. 2006 Aug 18;281(33):23733-9.

"Host tissue damage" signal ATP promotes non-directional migration and negatively regulates toll-like receptor signaling in human monocytes.
Kaufmann A, Musset B, Limberg SH, Renigunta V, Sus R, Dalpke AH, Heeg KM, Robaye B, Hanley PJ.
J Biol Chem. 2005 Sep 16;280(37):32459-67.

K(ATP) channels and preconditioning: a re-examination of the role of mitochondrial K(ATP) channels and an overview of alternative mechanisms.
Hanley PJ, Daut J.
J Mol Cell Cardiol. 2005 Jul;39(1):17-50.

Enantioselective actions of bupivacaine and ropivacaine on coronary vascular resistance at cardiotoxic concentrations.
Burmester MD, Schlüter KD, Daut J, Hanley PJ.
Anesth Analg. 2005 Mar;100(3):707-12.

5-Hydroxydecanoate is metabolised in mitochondria and creates a rate-limiting bottleneck for beta-oxidation of fatty acids.
Hanley PJ, Dröse S, Brandt U, Lareau RA, Banerjee AL, Srivastava DK, Banaszak LJ, Barycki JJ, Van Veldhoven PP, Daut J.
J Physiol. 2005 Jan 15;562(Pt 2):307-18.

Excitation-contraction coupling in the heart and the negative inotropic action of volatile anesthetics.
Hanley PJ, ter Keurs HE, Cannell MB.
Anesthesiology. 2004 Oct;101(4):999-1014.

Extracellular ATP induces oscillations of intracellular Ca2+ and membrane potential and promotes transcription of IL-6 in macrophages.
Hanley PJ, Musset B, Renigunta V, Limberg SH, Dalpke AH, Sus R, Heeg KM, Preisig-Müller R, Daut J.
Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9479-84.

Species-independent metabolic response to an increase of [Ca(2+)](i) in quiescent cardiac muscle.
Ward ML, Cooper PJ, Hanley PJ, Loiselle DS.
Clin Exp Pharmacol Physiol. 2003 Aug;30(8):586-9.

Beta-oxidation of 5-hydroxydecanoate, a putative blocker of mitochondrial ATP-sensitive potassium channels.
Hanley PJ, Gopalan KV, Lareau RA, Srivastava DK, von Meltzer M, Daut J.
J Physiol. 2003 Mar 1;547(Pt 2):387-93.

Extracellular Ca2+ is obligatory for ouabain-induced potentiation of cardiac basal energy expenditure.
Guild SJ, Ward ML, Cooper PJ, Hanley PJ, Loiselle DS.
Clin Exp Pharmacol Physiol. 2003 Jan-Feb;30(1-2):103-9.

Interaction with 14-3-3 proteins promotes functional expression of the potassium channels TASK-1 and TASK-3.
Rajan S, Preisig-Müller R, Wischmeyer E, Nehring R, Hanley PJ, Renigunta V, Musset B, Schlichthörl G, Derst C, Karschin A, Daut J.
J Physiol. 2002 Nov 15;545(Pt 1):13-26.

Halothane, isoflurane and sevoflurane inhibit NADH:ubiquinone oxidoreductase (complex I) of cardiac mitochondria.
Hanley PJ, Ray J, Brandt U, Daut J.
J Physiol. 2002 Nov 1;544(Pt 3):687-93.

Four novel splice variants of sulfonylurea receptor 1.
Hambrock A, Preisig-Müller R, Russ U, Piehl A, Hanley PJ, Ray J, Daut J, Quast U, Derst C.
Am J Physiol Cell Physiol. 2002 Aug;283(2):C587-98.

K(ATP) channel-independent targets of diazoxide and 5-hydroxydecanoate in the heart.
Hanley PJ, Mickel M, Löffler M, Brandt U, Daut J.
J Physiol. 2002 Aug 1;542(Pt 3):735-41.

Long-chain fatty acids increase basal metabolism and depolarize mitochondria in cardiac muscle cells.
Ray J, Noll F, Daut J, Hanley PJ.
Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1495-501.

Long-chain acyl-coenzyme A esters and fatty acids directly link metabolism to K(ATP) channels in the heart.
Liu GX, Hanley PJ, Ray J, Daut J.
Circ Res. 2001 May 11;88(9):918-24.

Metabolic consequences of a species difference in Gibbs free energy of Na+/Ca2+ exchange: rat versus guinea pig.
Cooper PJ, Ward ML, Hanley PJ, Denyer GR, Loiselle DS.
Am J Physiol Regul Integr Comp Physiol. 2001 Apr;280(4):R1221-9.

3-Dimensional configuration of perimysial collagen fibres in rat cardiac muscle at resting and extended sarcomere lengths.
Hanley PJ, Young AA, LeGrice IJ, Edgar SG, Loiselle DS.
J Physiol. 1999 Jun 15;517 ( Pt 3):831-7.

Mechanisms of force inhibition by halothane and isoflurane in intact rat cardiac muscle.
Hanley PJ, Loiselle DS.
J Physiol. 1998 Jan 1;506 ( Pt 1):231-44.