The Institute for Physical Chemistry at the University of Münster, Germany, is seeking to fill the post of a
(salary level TV-L E13, 100%)
in biochemistry/biophysical chemistry
The position is within the group of Prof Dr Dagmar Klostermeier, and it is funded within the framework of the Collaborative Research Center 858. The fixed-term post is available immediately, and will last until 31 December 2019. Currently, the regular working time for full employment is 39 hours and 50 minutes per week. All posts can, as a rule, also be filled as part-time positions if there are no compelling work-related reasons against doing so.
The position involves investigating the mechanism of positive DNA supercoiling by reverse gyrase. Reverse gyrase is a DNA topoisomerase of thermophilic and hyperthermophilic bacteria that catalyzes the ATP-dependent introduction of negative supercoils into DNA. Reverse gyrase consists of a helicase domain and a topoisomerase domain that cooperate functionally in DNA binding and processing during the supercoiling reaction. The successful applicant will investigate the molecular basis for the functional interaction of the helicase and topoisomerase domains in reverse gyrase. To this end, he/she will analyze DNA binding to the helicase domain and the topoisomerase domain. Specifically, the successful applicant will perform single molecule FRET experiments to identify DNA-induced conformational changes and then correlate these changes with DNA binding and supercoiling activity.
The Institute for Physical Chemistry offers state-of-the-art molecular biology and biochemistry laboratories, as well as confocal single molecule microscopes with alternating laser excitation and total internal reflection molecule microscopes with fast camera detection systems.
The successful applicant must hold a PhD degree in biochemistry, chemistry, biology, biophysics or related fields and must have a genuine interest in research, combined with experimental enthusiasm. Experience with molecular biology, protein and nucleic acid preparation, and single molecule microscopy is required.
The University of Münster is an equal opportunity employer and is committed to increasing the proportion of women academics. Consequently, we actively encourage applications by women. Female candidates with equivalent qualifications and academic achievements will be preferentially considered within the framework of the legal possibilities. We also welcome applications from candidates with severe disabilities. Disabled candidates with equivalent qualifications will be preferentially considered.
If you want to work in a stimulating environment as part of an international and interdisciplinary team, please send your application including CV, list of publications, letter of motivation, and two references as a single PDF file by 31 January 2018 to:
Prof Dr Dagmar Klostermeier
University of Münster
Institute for Physical Chemistry