Violette Phosphatasen und Catecholoxidasen:
Kristallisation, Röntgenstrukturanalyse und Sequenzbestimmung

Isolierung, Charakterisierung, Kristallisation und Röntgenstrukturanalyse von tierischen und pflanzlichen violetten sauren Phosphatasen, Catecholoxidasen,Tyrosinasen und Metallothioneinen. Spektroskopische und kinetische Untersuchungen zu den Reaktionsmechanismen. Molekularbiologische Arbeiten zur Erzeugung von Punktmutanten für funktionelle und mechanistische Untersuchungen.

Beteiligte Wissenschaftler:

Prof. Dr. Bernt Krebs (Leiter), Priv.-Doz. Annette Rompel, Dr. Michael Albers, Dr. Atila Durmus, Dipl.-Chem. Carsten Gerdemann, Dipl.-Chem. Jens Greving, Dipl.-Chem. Joseph Itor Naseri, Dipl.-Chem. Thanh Truong , Dipl.-Chem. Andreas Vogel, Dagmar König, Beate Jasper

Veröffentlichungen:

A. Rompel, H. Fischer, D. Meiwes, K. Büldt-Karentzopoulos, R. Dillinger, F. Tuczek, H. Witzel u. B. Krebs: Purification and spectroscopic studies on catechol oxidases from Lycopus europaeus and Populus nigra: Evidences for a dinuclear copper center of type 3 and spectroscopic similarities to tyrosinase and hemocyanin, JBIC 4, 56 (1999)

A. Durmus, C. Eicken, B. Sift, A. Kratel, R. Kappl, J. Hüttermann u. B. Krebs: The active site of purple acid phosphatase from sweet potatoes (Ipomoea batatas): Metal content and spectroscopic characterization, Eur. J. Biochem. 260, 709 (1999)

B. H. Sift, A. Durmus, W. Meyer-Klaucke u. B. Krebs: EXAFS Studies on the Active Site of Purple Acid Phosphatase from Sweet Potato Ipomoea batatas, J. Synchr. Rad. 6, 421 (1999)

A. Rompel, H. Fischer, D. Meiwes, K. Büldt-Karentzopoulos, A. Magrini, C. Eicken, C. Gerdemann u. B. Krebs: Substrate specificity of catechol oxidase from Lycopus europaeus and characterization of the bioproducts of enzymic caffeic acid oxidation, FEBS Letters 445, 103 (1999)

A. Durmus, C. Eicken, F. Spener u. B. Krebs: Cloning and comparative protein modeling of two purple acid phosphatase isozymes from sweet potatoes (Ipomoea batatas), BBA - Protein Struct. & Mol. Enzymol. 1434, 202 (1999)

A. Rompel, R. Locke, M. Pascaly, H. Witzel u. B. Krebs: Purple acid phosphatase from red kidney beans (KBPAP): catalytic reduction and oxidation of the chromophoric Fe(III) site, Biochim. Biophys. Acta (in preparation)

C. Gerdemann, C. Eicken, A. Magrini, H. E. Meyer, A. Rompel, F. Spener u. B. Krebs: Isozymes of Ipomea batatas catechol oxidase differ in catalase-activity, Biochem. Biophys. Acta (submitted)

C. Gerdemann, C. Eicken u. B. Krebs: The crystal structure of catechol oxidase: New insight into the function of copper type-3 proteins, Acc. Chem. Res. (submitted)

A. Vogel, P. Schnittger, T. Börchers, B. Krebs u. F. Spener: Cloning of a cDNA encoding purple acid phosphatase from red kidney bean, Biochim. Biophys. Acta (in preparation)

B. H. Sift, A. Durmus, W. Meyer-Klaucke u. B. Krebs: Photoreduction of the active sites of purple acid phosphatases by high-energy synchrotron radiation, EMBL Annual Report 411 (1998)

C. Eicken, B. Krebs u. J. C. Sacchettini: Catechol Oxidase - Structure and Activity, Curr. Opin. in Struct. Biol. 9, 677 (1999)

B. Krebs, C. Eicken, C. Gerdemann, K. Büldt-Karentzopoulos, A. Magrini, T. Klabunde u. J. C. Sacchettini: Crystal structure, sequence, and mechanism of catechol oxidase, J. Inorg. Biochem. 74, 35 (1999)

A. Vogel, F. Spener u. B. Krebs: Purple Acid Phosphatase, Buchbeitrag in: "Handbook of Metalloproteins" (accepted)

A. Rompel, C. Gerdemann, A. Vogel u. B. Krebs: Enzyme structures: Active site structural and functional aspects of purple acid phosphatase and catechol oxidase, Buchbeitrag in: "Inorganic Chemistry Highlights" (accepted)

C. Eicken, C. Gerdemann u. B. Krebs: Catechol Oxidase, Buchbeitrag in: "Handbook of Metalloproteins" (accepted)

A. Vogel, P. Schnittger, T. Börchers, K. Markus, B. Krebs u. F. Spener: Heterologous expression and characterisation of a seed specific purple acid phosphatase from red kidney bean (in preperation)