Dr. Andreas Gratz

Wissenschaftlicher Mitarbeiter

andreas.gratz@uni-muenster.de

Research

• Head of the Autodisplay screening subgroup
• Bioaffinity experiments using Autodisplay, flow cytometry and bacterial cell sorting
• Development of peptide inhibitors of human protein kinase CK2
• Autodisplay of human protein kinase CK2
• Development and application of assays for protein kinase CK2 (i.e. capillary electrophoresis)
• Directed Evolution and characterization of esterase EstE (finished)

Teaching

• Head of Biochemistry course for pharmacy students

Publications

Original research

• Götz C, Gratz A, Kucklaender U, Jose J (2012) TF – a novel cell-permeable and selective inhibitor of human protein kinase CK2 induces apoptosis in the prostate cancer cell line LNCaP. Biochim Biophys Acta - General subjects. DOI:10.1016/j.bbagen.2012.02.009
• Gratz A, Bollig R, Kuckländer U, Götz C, Jose J (2011): Identification of novel CK2 inhibitors with a benzofuran scaffold by novel non-radiometric in vitro assays. Molecular and Cellular Biochemistry, online first, DOI: 10.1107/s11010-011-0957-4.
• Kucklaender U, Bollig R, Frank W, Gratz A, Jose J (2011); A novel application of DDQ as electrophile in the Nenitzescu reaction. Bioorganic & Medicinal Chemistry, 19:2666-74
• Bouaziz Z , Issa S, Le Borgne M, Jose J, Gratz A, Götz C, Di Pietro A, Falson P, Arnaud O (2010); Targeting casein kinase 2 in drug discovery: identification of new chemical entities. Bulletin du Cancer, 97:68-9
• Gratz A, Götz,C, Jose J (2010); A CE-based assay for human protein kinase CK2 activity measurement and inhibitor screening. Electrophoresis, 31:634-40
• Gratz A, Götz C, Jose J (2010); A FRET-based microplate assay for human protein kinase CK2, a target in neoplastic disease. 
Journal of Enzyme Inhibition and Medicinal Chemistry, 25:234-9
• Gratz A, Jose J (2008); Protein Domain Library Generation by Overlap extension (PDLGO): a tool for enzyme engineering. Analytical Biochemistry, 378:171-6

Patent applications

• Gratz A, Kuckländer U, Götz C, Jose J (2010) Verwendung von Benzofuranonderivaten zur Inhibierung von Kinasen. DE102010025173.9.

Books and Chapters

• Gratz A, Jose J; Focusing mutations within random libraries to distinct areas: Protein domain library generation by overlap extension (PDLGO). In Methods in Molecular Biology Vol. 729: cDNA Libraries: Methods and Applications, Browse, J., Lu, C. and Wallis, J.G., Eds., Humana Press, 2011, ISBN: 978-1-61779-064-5
• Gratz, A (2010); Autodisplay der humanen Proteinkinase CK2 und Entwicklung eines Verfahrens zur Inhibitionstestung, Logos Verlag, Berlin, ISBN: 978-3-8325-2573-6

Oral Presentations

• Jose J, Gratz A, Götz C (2010); Capillary electrophoresis (CE) and FRET as tools for testing inhibitors of human protein kinase CK2. DPhG joint meeting, 4th-7th october 2010, Braunschweig (F2-5)
• Gratz A, Götz C, Jose J (2010); Towards the development of non-radiometric in vitro assays for protein kinase CK2. 6th International Conference on Protein Kinase CK2, 7th-10th september, Köln.
• Gratz A, Götz C, Jose J (2009); Development of a CE based assay for evaluation of human protein kinase CK2 inhibitors. DPhG Doktorandentagung 18th-21th november, Pichlarn-Aigen
• Gratz A, Götz C, Jose J (2009); A novel in vitro assay for human protein kinase CK2 inhibitors based on capillary electrophoresis with UV detection. XVIIIth European Conference of GP2A, 3rd-4th september Trinity College Dublin (R5).
• Gratz A, Jose J (2006); A new method for directed evolution: Protein domain library generation by overlap extension (PDLGO). DPhG Doktorandentagung, 6th-8th september, Nürnberg-Heroldsberg (V 2.7).

Posters

• Goblirsch M, Gratz A, Jose J (2012) Comparing Tat- and Sec-mediated autodisplay of GFP. DPhG-Doktorandentagung, Weimar, November 14-17. P7
• Reicheneder C, Gratz A, Jose J (2012) Bacterial surface display library screening for the identification of potent non-ATP-competitive CK2 inhibitors. DPhG-Doktorandentagung, Weimar, November 14-17. P20
• Reicheneder C, Gratz A, Jose J (2012) Identification of a peptidic non-ATP competitive inhibitor of human CK2 by bacterial surface display library screening. XXIInd International Symposium on Medicinal Chemistry, Berlin, September 3-6. Poster 138
• Bollacke A, Hundsdörfer C, Gratz A, Jose J (2012) Autodisplay of human protein kinase CK2 for inhibitor testing. XXIInd International Symposium on Medicinal Chemistry, Berlin, September 3-6. Poster 148
• Gratz A, Götz C, Kuckländer U, Jose J (2012); Discovery of selective and cell-permeable inhibitos of Ck2, inducing apoptosis in cancer cells. Forschung der chemischen Institute (Symposium), 19.4.2012, WWU Münster, Poster 27
• Al Chab F, Marminon C, Bouaziz Z, Ettouati L, Gentili J, Nebois P, Gratz A, Hundsdörfer C, Jose J, Le Borgne M (2011); Optimization of biomolecules targeting Protein Kinase CK2. 16ème Journée Scientifique de l'EDISS – Ecole Doctorale Interdisciplinaire Sciences Santé, 24th march, Lyon, Poster P53
• Gratz A, Götz C, Kuckländer U, Jose J (2010); Benzofuranone TF inhibits protein kinase CK2 and shows pro-apoptotic effects in prostate cancer cells. DPhG Jahrestagung, 4th-7th october 2010, Braunschweig.
• Gratz A, Götz C, Jose J (2010); Towards the development of non-radiometric in vitro assays for protein kinase CK2. 6th International Conference on Protein Kinase CK2, 7th-10th september, Köln.
• Gratz A, Bollig R, Kuckländer U, Jose J (2010); Benzofuran derivatives as attractive CK2 inhibitors. 6th International Conference on Protein Kinase CK2, 7th-10th september, Köln.
• Gratz A, Götz C, Kuckländer U, Jose J (2010); Identification of a benzofuranone derivative as powerful inhibitor of CK2 and its effect on apoptosis. 6th International Conference on Protein Kinase CK2, 7th-10th september, Köln.
• Marminon C, Nebois P, Gratz A, Kassack M, Jose J, Le Borgne, M (2010); Exploring new scaffolds for CK2 inhibitory activity: two case studies. XIXth European Conference of GP2A, 1st-2nd september, Rouen.
• Bouaziz Z, Issa S, Jose J, Gratz A, Götz C, Di Pietro A, Falson P, Arnaud O, Le Borgne M (2010); Targeting casein kinase 2 in drug discovery: identification of new chemical entities. 5^èmes Journées Scientifiques du CLARA, 30th-31th march, Lyon, Poster A735ZB.
• Gratz A, Bollig R, Kuckländer U, Jose J (2010); Identification of novel human protein kinase CK2 inhibitors. Frontiers in Medicinal Chemistry, 14th-17th march, Münster, Poster HMC59.
• Gratz A, Götz C, Jose J (2009); A non-radiometric inhibitor test for human protein kinase CK2 based on capillary electrophoresis with UV detection. 
DPhG joint meeting, 28th september-1st october, Jena, Poster A93.
• Gratz A, Götz C, Maas R, Jose J (2008); Peptides on a bug: fluorescence activated cell screening of potential human protein kinase CK2 inhibitor libraries. DPhG Jahrestagung, 8th-11th october, Bonn, Poster A28.
• Gratz A, Jose J (2007); Ein neues Werkzeug für die gerichtete Evolution von Proteindomänen: Protein Domain Library Generation by Overlap Extension (PDLGO). Tag des wissenschaftlichen Nachwuchses, 22th june, Heinrich-Heine-Universität Düsseldorf , Konferenzband 2007, S. 144-145.
• Gratz A, Jose J (2006); PDLGO: a novel tool for directed evolution of protein domains. DPhG Jahrestagung, 4th-7th october, Marburg, Poster P6.