Research Interests

  • DNA with Metal-Mediated Base Pairs

Metal-mediated base pairs represent a methodology for the site-specific incorporation of metal-based functionality into nucleic acids. In metal-mediated base pairs, the metal ions are located inside the double helix. This unusual localization is accomplished by using artificial base surrogates capable of binding metal ions, hence by a formal substitution of hydrogen bonds within a base pair by coordinative bonds. We are designing artificial nucleobases to be used in is this kind of bio-inspired self-assembling array of metal ions. Moreover, we are developing applications for nucleic acids with metal-mediated base pairs.

See also:
Nucleic Acids With Metal-Mediated Base Pairs and Their Applications (Review article)
A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases
A Dinuclear Mercury(II)-Mediated Base Pair in DNA
Formation of Silver Nanoclusters from a DNA Template Containing Ag(I)-Mediated Base Pairs
Single-Molecule Force Spectroscopy of an Artificial DNA Duplex Comprising a Silver(I)-Mediated Base Pair
Cooperative formation of silver(I)-mediated base pairs
Contiguous Metal-Mediated Base Pairs Comprising Two Ag(I) Ions
Solution structure of a DNA double helix with consecutive metal-mediated base pairs
Bipyridine-modified oligonucleotides: Aggregation in the presence of metal ions
Using In Vitro Transcription to Construct Scaffolds for One-Dimensional Arrays of Mercuric Ions
An Artificial Base Pair, Mediated by Hydrogen Bonding and Metal-Ion Binding
Metals line up for DNA (News & Views article in Nature)

  • Stabilisation of parallel stranded DNA with metals and metal complexes

Parallel stranded (ps) DNA is an emerging new feature of DNA structure. It is usually characterised by the occurrence of reverse Watson Crick base pairs or homo base pairs and mostly displays a trans-orientation of the glycosidic bonds. Only few examples are known with Hoogsteen base pairing, which at the same time provide the only examples with a cisoid arrangement of the glycosidic bonds.
We are interested in factors stabilizing ps DNA, and in particular in the question whether metal-mediated base pairs are capable of stabilizing ps DNA.

See also:
Relative Strand Orientation in a DNA Duplex Controls the Nuclearity of a Metal-Mediated Base Pair
A Dinuclear Mercury(II)-Mediated Base Pair in DNA
A Highly Stabilizing Silver(I)-Mediated Base Pair in Parallel-Stranded DNA
Parallel stranded DNA with Hoogsteen base pairing: characterization and conversion into a homodimer and a triplex

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