Academics at the Department of Biology

Wissenschaftlicher Werdegang
- 1971-1975 Studium Biologie/Chemie Ruhr-Universität Bochum
Stipendiat der Kurt-Hansen-Stiftung
- 1975/76 Staatsexamen Lehramt an Gymnasien
- 1978 Promotion (Dr. rer. nat.), Ruhr-Universität Bochum
- 1983 Habilitation (Botanik), Ruhr-Universität Bochum
- 1986/88 Professur (C3) für Mikrobiologie, Universität Düsseldorf
- seit 1988 Professur (C4) für Allg. Botanik/Mikrobiologie, Institut für Botanik, Westf. Wilhelms-Universität Münster

Lehrschwerpunkte
- Allgemeine Botanik
- Biologie der Pilze und niederen Pflanzen
- Genetik, Molekularbiologie und Biotechnologie der Pilze
- Molekulare Phytopathologie

Forschungsschwerpunkte
- Biotechnologie: Molekularbiologie der pilzlichen Sekundärstoffsynthese
- Molekulare Phytopathologie: Pathogenitätsfaktoren phytopathogener Pilze

Ausgewählte Projekte

Molecular genetics of Ergot-Alkaloidbiosynthesis in Claviceps purpurea
(DFG Tu 50/13; SPP 1152 "Evolution of metabolic diversity"; Link: http://www.ipb-halle.de/dfg-spp-1152/homepage/index.htm
Ergot alkaloids are toxic ergoline derivatives, which are synthesized by several members of the ascomycetes family Clavicipitaceae, especially by Claviceps spec. The pharmacological relevant peptide alkaloids (ergopeptides) are produced e.g. by Claviceps purpurea, whereas other species synthesize only lysergic acid (LA) and simple LA derivatives (C. paspali) or only precursors of LA, ergoline alkaloids (C. fusiformis). We have recently discribed a cluster of genes involved in peptide alkaloid biosynthesis in C. purpurea; we could identify the genes coding for the non-ribosomal-peptide synthetases (NRPS) involved in the peptide moiety in an ergotamine producing strain. In this project the analysis of cluster genes will be continued . We will focus on functional analysis of NRPS genes, in comparison between C. purpurea strains with different alkaloid spectra, to investigate the basis for diversity of peptide alkaloids within the species C. purpurea ("chemical races"). The next step -based on these informations - will be the analysis of alkaloid genes (presence and function) in different Claviceps species (C. paspali, C. fusiformis) to study the evolution of the biosynthetic pathways of ergot alkaloids within the genus Claviceps.

Early stages of pathogenicity in plant pathogenic fungi
(Trilateral DFG program: cooperation with Tel Aviv and Hebron University)
Plant pathogenic fungi devised various strategies to infect plants. Regardless of the pathogenic life style, the initial stages of infection are common to most fungi: spores germinate on the host surface, undergo a short period of polarized growth and then invade the plant tissue. These early events are crucial for the development of disease and hence are strictly regulated at several levels. We study these early processes in two fungi with different pathogenic life styles: the hemibiotroph Colletotrichum gloeosporioides and the necrotroph Botrytis cinerea. We generated and characterized mutants with defects in various stages of early pathogenic development and use such mutants to determine the role of signal pathways in regulation of early pathogenic development. The results will provide better understanding of these complex interaction systems, and could constitute a basis for the development of alternative disease control systems (definition of targets). We use an integrated approach combining classical (physiology, cytology) and molecular techniques (including array and imaging technology). The 3 partner labs contribute to this research specific expertise and work in close collaboration, including intensive transfer of knowledge and data between the partners, leading to a substantial broadening of the expertise of all partners with special focus on the Hebron lab.

Role of reactive oxygen species (ROS) in the interaction of phytopathogenic fungi and their hosts (DFG project)
ROS play a major role in defense reactions of plants and animals against pathogens. Phytopathogenic fungi face considerable oxidative stress by the so-called "oxidative burst", an early plant defense reaction. We could show that phytopathogenic fungi not only possess effective ROS-detoxification systems, but that they can produce ROS themselves and thus contribute to the ROS status during the interaction. We study two interaction systems showing highly different strategies: Claviceps purpurea, a biotrophic pathogen of rye, obviously has adapted to his host at a high degree, since formation of plant-derived and self-produced ROS is minimized. The necrotrophic grey mould Botrytis cinerea, on the other hand, stimulates the "oxidative burst" and contributes significant amounts of ROS, causing death of the infected tissue. We are analysing in parallel ROS production and detoxification in both systems, focusing on signal chains and ROS-generating systems. We want to test the hypothesis that both fungi use the same system compounds to achieve these different strategies, and we want to prove that the differences in strategy can be recognized mainly at the level of ROS generating and detoxifying systems.

Molecular Interactions of Pathogens with Biotic and Abiotic Surfaces (International Graduate School, GRK 1409; http://zmbe.uni-muenster.de/GRK1409/
Perception and transduction of signals is essential for early infection stages of phytopathogenic fungi. Genes expressed in the early stages (germination of spores on the plant surface, penetration of cuticle, start of colonization) will be identified e.g. by array analyses, using defined mutants of essential signalling pathways. Candidate genes will be functionally analysed by gene replacement and reporter gene analyses. The studies will be performed in close context with the major projects of the group, within one of the two major systems: Claviceps purpurea, the Ergot fungus, on rye, or Botrytis cinerea, the grey mould, on bean. Beyond the whole set of classical molecular genetic techniques, the project will include fungal transformation (gene replacement, reporter gene arrays), protein techniques (western, phosphorylation analyses), array technology and bioinformatics (for B. cinerea a genome sequence is available).

Ausgewählte Kooperationen
- Dr. Marc-Henri Lebrun, Bayer Crop Science, Lyon, Frankreich
- Dr. Jan van Kan, Wageningen University, Niederlande
- Prof. Dr. Christopher L. Schardl, University of Lexington, USA
- Prof. Dr. Ullrich Keller, TU Berlin, Deutschland (alkaloid biosynthesis, C. purpurea)
- Dr. Amir Sharon, Tel Aviv University, Israel (early infection stages, B. cinerea)
- Dr. Ben Horwitz, Technion, Haifa, Israel (reactive oxygen species, B. cinerea)
- Prof. Dr. Radwan Barakat, Hebron University, Palästina (pathogenicity B. cinerea)
- Dr. Sabine Fillinger, INRA Versailles, Frankreich.

Publications

  • Schürmann J, Buttermann D, Herrmann A, Giesbert S, Tudzynski P. . ‘Molecular characterization of the NADPH oxidase complex in the Ergot fungus Claviceps purpurea: CpNox2 and CpPls1 are important for a balanced host-pathogen interaction.Molecular plant-microbe interactions : MPMI . doi: 10.1094/MPMI-03-13-0064-R.
  • Schumacher J, Tudzynski P. . ‘Morphogenesis and infection in Botrytis cinerea.’ In Topics in Current Genetics, Vol. Morphogenesis and Pathogenicity in Fungi, edited by Pérez-Martín J, Di Pietro A (Eds.): , 225-241. doi: 10.1007/978-3-642-22916-9_11.
  • Giesbert S, Schumacher J, Kupas V, Espino J, Segmüller N, Haeuser-Hahn I, Schreier PH, Tudzynski P. . ‘Identification of pathogenesis-associated genes by T-DNA-mediated insertional mutagenesis in Botrytis cinerea: A type 2A phosphoprotein phosphatase and an SPT3 transcription factor have significant impact on virulence.’ Molecular Plant-Microbe Interactions 25, No. 4: 481-495. doi: 10.1094/MPMI-07-11-0199.
  • Roca MG, Weichert M, Siegmund U, Tudzynski P, Fleißner A. . ‘Germling fusion via conidial anastomosis tubes in the grey mould Botrytis cinerea requires NADPH oxidase activity.’ Fungal Biology 116, No. 3: 379-387. doi: 10.1016/j.funbio.2011.12.007.
  • Heller J, Ruhnke N, Espino J, Massaroli M, Collado IG, Tudzynski P. . ‘The MAP kinase BcSak1 of Botrytis cinerea is required for pathogenic development and has broad regulatory functions beyond stress response.Molecular plant-microbe interactions : MPMI 25. doi: 10.1094/MPMI-11-11-0299.
  • Temme N, Oeser B, Massaroli M, Heller J, Simon A, González Collado I, Viaud M, Tudzynski P. . ‘BcAtf1, a global regulator, controls various differentiation processes and phytotoxin production in Botrytis cinerea.Molecular plant pathology 13. doi: 10.1111/j.1364-3703.2011.00778.x.
  • Heller J, Tudzynski P. . ‘Reactive oxygen species in phytopathogenic fungi: signaling, development, and disease.Annual review of phytopathology 49: 369-90. doi: 10.1146/annurev-phyto-072910-095355.
  • Nesher I, Minz A, Kokkelink L, Tudzynski P, Sharon A. . ‘Regulation of pathogenic spore germination by cgrac1 in the fungal plant pathogen colletotrichum gloeosporioides.’ Eukaryotic Cell 10, No. 8: 1122-1130. doi: 10.1128/EC.00321-10.
  • Jonkers W, Van Kan JAL, Tijm P, Lee Y-W, Tudzynski P, Rep M, Michielse CB. . ‘The FRP1 F-box gene has different functions in sexuality, pathogenicity and metabolism in three fungal pathogens.’ Molecular Plant Pathology 12, No. 6: 548-563. doi: 10.1111/j.1364-3703.2010.00689.x.
  • Kokkelink L, Minz A, Al-Masri M, Giesbert S, Barakat R, Sharon A, Tudzynski P. . ‘The small GTPase BcCdc42 affects nuclear division, germination and virulence of the gray mold fungus Botrytis cinerea.’ Fungal Genetics and Biology 48, No. 11: 1012-1019. doi: 10.1016/j.fgb.2011.07.007.
  • Amselem J, Cuomo CA, van Kan JAL, Viaud M, Benito EP, Couloux A, Coutinho PM, de Vries RP, Dyer PS, Fillinger S, Fournier E, Gout L, Hahn M, Kohn L, Lapalu N, Plummer KM, Pradier J-M, Quévillon E, Sharon A, Simon A, Have A, Tudzynski B, Tudzynski P, Wincker P, Andrew M, Anthouard V, Beever RE, Beffa R, Benoit I, Bouzid O, Brault B, Chen Z, Choquer M, Collémare J, Cotton P, Danchin EG, Da Silva C, Gautier A, Giraud C, Giraud T, Gonzalez C, Grossetete S, Güldener U, Henrissat B, Howlett BJ, Kodira C, Kretschmer M, Lappartient A, Leroch M, Levis C, Mauceli E, Neuvéglise C, Oeser B, Pearson M, Poulain J, Poussereau N, Quesneville H, Rascle C, Schumacher J, Ségurens B, Sexton A, Silva E, Sirven C, Soanes DM, Talbot NJ, Templeton M, Yandava C, Yarden O, Zeng Q, Rollins JA, Lebrun M-H, Dickman M. . ‘Genomic analysis of the necrotrophic fungal pathogens sclerotinia sclerotiorum and botrytis cinerea.’ PLoS Genetics 7, No. 8. doi: 10.1371/journal.pgen.1002230.
  • Giesbert S, Schumacher J, Kupas V, Espino J, Segmueller N, Haeuser-Hahn I, Schreier P, Tudzynski P. . ‘Identification of pathogenesis associated genes by T-DNA-mediated insertional mutagenesis in Botrytis cinerea: a type 2A phosphoprotein phosphatase and a SPT3 transcription factor have significant impact on virulence.Molecular plant-microbe interactions : MPMI . doi: 10.1094/MPMI-07-11-0199.
  • Michielse CB, Becker M, Heller J, Moraga J, Collado IG, Tudzynski P. . ‘The Botrytis cinerea Reg1 protein, a putative transcriptional regulator, is required for pathogenicity, conidiogenesis, and the production of secondary metabolites.Molecular plant-microbe interactions : MPMI 24, No. 9: 1074-85. doi: 10.1094/MPMI-01-11-0007.
  • Lorenz N, Olsovska J, Sulc M, Tudzynski P. . ‘Alkaloid Cluster Gene ccsA of the Ergot Fungus Claviceps purpurea Encodes Chanoclavine I Synthase, a Flavin Adenine Dinucleotide-Containing Oxidoreductase Mediating the Transformation of N-Methyl-Dimethylallyltryptophan to Chanoclavine I.’ APPLIED AND ENVIRONMENTAL MICROBIOLOGY 76, No. 6: 1822-1830. doi: 10.1128/AEM.00737-09.
  • Bormann J, Tudzynski P. . ‘Deletion of Mid1, a putative stretch-activated calcium channel in Claviceps purpurea, affects vegetative growth, cell wall synthesis and virulence.’ MICROBIOLOGY-SGM 155: 3922-3933. doi: 10.1099/mic.0.030825-0.
  • Lorenz N, Haarmann T, Pazoutova S, Jung M, Tudzynski P. . ‘The ergot alkaloid gene cluster: Functional analyses and evolutionary aspects.’ PHYTOCHEMISTRY 70, No. 15-16: 1822-1832. doi: 10.1016/j.phytochem.2009.05.023.
  • Lorenz N, Haarmann T, Pazoutová S, Jung M, Tudzynski P. . ‘The ergot alkaloid gene cluster: functional analyses and evolutionary aspects.Phytochemistry 70, No. 15-16: 1822-32. doi: 10.1016/j.phytochem.2009.05.023.
  • Oeser B, Beaussart F, Haarmann T, Lorenz N, Nathues E, Rolke Y, Scheffer J, Weiner J, Tudzynski P. . ‘Expressed sequence tags from the flower pathogen Claviceps purpurea.’ MOLECULAR PLANT PATHOLOGY 10, No. 5: 665-684. doi: 10.1111/j.1364-3703.2009.00560.x.
  • Temme N, Tudzynski P. . ‘Does Botrytis cinerea Ignore H2O2-Induced Oxidative Stress During Infection? Characterization of Botrytis Activator Protein 1.’ MOLECULAR PLANT-MICROBE INTERACTIONS 22, No. 8: 987-998. doi: 10.1094/MPMI-22-8-0987.
  • Haarmann T, Rolke Y, Giesbert S, Tudzynski P. . ‘Ergot: from witchcraft to biotechnology.Molecular plant pathology 10, No. 4: 563-77. doi: 10.1111/j.1364-3703.2009.00548.x.
  • Haarmann T, Rolke Y, Giesbert S, Tudzynski P. . ‘Ergot: from witchcraft to biotechnology.’ MOLECULAR PLANT PATHOLOGY 10, No. 4: 563-577. doi: 10.1111/j.1364-3703.2009.00548.x.
  • Tudzynski P, Kokkelink L. . ‘Botrytis cinerea: molecular aspects of a necrotrophic life-style.Springer 5, No. 1: 22. doi: 10.1007/978-3-540-87407-2_2.
  • Schumacher J, Kokkelink L, Huesmann C, Jimenez-Teja D, Collado IG, Barakat R, Tudzynski P, Tudzynski B. . ‘The cAMP-Dependent Signaling Pathway and Its Role in Conidial Germination, Growth, and Virulence of the Gray Mold Botrytis cinerea.’ MOLECULAR PLANT-MICROBE INTERACTIONS 21, No. 11: 1443-1459. doi: 10.1094/MPMI-21-11-1443.
  • Segmuller N, Kokkelink L, Giesbert S, Odinius D, van Kan J, Tudzynski P. . ‘NADPH Oxidases are involved in differentiation and pathogenicity in Botrytis cinerea.’ MOLECULAR PLANT-MICROBE INTERACTIONS 21, No. 6: 808-819. doi: 10.1094/MPMI-21-6-0808.
  • Giesbert S, Schurg T, Scheele S, Tudzynski P. . ‘The NADPH oxidase Cpnox1 is required for full pathogenicity of the ergot fungus Claviceps purpurea.’ MOLECULAR PLANT PATHOLOGY 9, No. 3: 317-327. doi: 10.1111/j.1364-3703.2008.00466.x.
  • Rolke Y, Tudzynski P. . ‘The small GTPase Rac and the p21-activated kinase Cla4 in Claviceps purpurea: interaction and impact on polarity, development and pathogenicity.’ MOLECULAR MICROBIOLOGY 68, No. 2: 405-423. doi: 10.1111/j.1365-2958.2008.06159.x.
  • Haarmann T, Lorenz N, Tudzynski P. . ‘Use of a nonhomologous end joining deficient strain (Delta ku70) of the ergot fungus Claviceps purpurea for identification of a nonribosomal peptide synthetase gene involved in ergotamine biosynthesis.’ FUNGAL GENETICS AND BIOLOGY 45, No. 1: 35-44. doi: 10.1016/j.fgb.2007.04.008.
  • Lorenz N, Wilson EV, Machado C, Schardl CL, Tudzynski P. . ‘Comparison of ergot alkaloid biosynthesis gene clusters in Claviceps species indicates loss of late pathway steps in evolution of C-fusiformis.’ APPLIED AND ENVIRONMENTAL MICROBIOLOGY 73, No. 22: 7185-7191. doi: 10.1128/AEM.01040-07.
  • Nathues E, Jorgens C, Lorenz N, Tudzynski P. . ‘The histidine kinase CpHK2 has impact on spore germination, oxidative stress and fungicide resistance, and virulence of the ergot fungus Claviceps purpurea.’ MOLECULAR PLANT PATHOLOGY 8, No. 5: 653-665. doi: 10.1111/j.1364-3703.2007.00421.x.
  • Williamson B, Tudzynsk B, Tudzynski P, van Kan JAL. . ‘Botrytis cinerea: the cause of grey mould disease.’ MOLECULAR PLANT PATHOLOGY 8, No. 5: 561-580. doi: 10.1111/j.1364-3703.2007.00417.x.
  • Segmuller N, Ellendorf U, Tudzynski B, Tudzynski P. . ‘BcSAK1, a stress-activated mitogen-activated protein kinase, is involved in vegetative differentiation and pathogenicity in Botrytis cinerea.’ EUKARYOTIC CELL 6, No. 2: 211-221. doi: 10.1128/EC.00153-06.
  • Williamson, B., Tudzynski, B., Tudzynski, P., and van Kan, J.A.L. . ‘Pathogen profile – Botrytis cinerea: the cause of grey mould disease.Molecular Plant Pathology 8, No. 5: 20. doi: 10.1111/j.1364-3703.2007.00417.x.
  • Siewers V, Kokkelink L, Smedsgaard J, Tudzynski P. . ‘Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea.’ APPLIED AND ENVIRONMENTAL MICROBIOLOGY 72, No. 7: 4619-4626. doi: 10.1128/AEM.02919-05.
  • Scheffer J, Tudzynski P. . ‘In vitro pathogenicity assay for the ergot fungus Claviceps purpurea.’ MYCOLOGICAL RESEARCH 110: 465-470. doi: 10.1016/j.mycres.2005.11.011.
  • Haarmann T, Ortel I, Tudzynski P, Keller U. . ‘Identification of the cytochrome P450 monooxygenase that bridges the clavine and ergoline alkaloid pathways.’ CHEMBIOCHEM 7, No. 4: 645-652. doi: 10.1002/cbic.200500487.
  • Chague V, Danit L, Siewers V, Gronover C, Tudzynski P, Tudzynski B, Sharon A. . ‘Ethylene sensing and gene activation in Botrytis cinerea: A missing link in ethylene regulation of fungus-plant interactions?MOLECULAR PLANT-MICROBE INTERACTIONS 19, No. 1: 19. doi: 10.1094/MPMI-19-0033.
  • Haarmann T, Ortel I, Tudzynski P, Keller U. . ‘The cytochrome P450 monooxygenase CpP450-1 is the bridge between clavine alkaloid and D-lysergic acid amid formation in the ergot alkaloid pathway in Claviceps purpurea.Chem. Biochem. 7, No. 4: 8. doi: 10.1002/cbic.200500487.
  • Schardl CL, Panaccione DG, Tudzynski P. . ‘Ergot alkaloids – biology and molecular biology.Alkaloids Chem. Biol. 63: 42. doi: 10.1016/S1099-4831(06)63002-2.
  • Scheffer J, Chen C, Heidrich P, Dickman MB, Tudzynski P. . ‘A CDC42 homologue in Claviceps purpurea is involved in vegetative differentiation and is essential for pathogenicity.Eukaryotic cell 4, No. 7: 1228-38. doi: 10.1128/EC.4.7.1228-1238.2005.
  • Siewers V, Viaud M, Jimenez-Teja D, Collado IG, Gronover CS, Pradier JM, Tudzynski B, Tudzynski P. . ‘Functional analysis of the cytochrome P450 monooxygenase gene bcbot1 of Botrytis cinerea indicates that botrydial is a strain-specific virulence factor.’ MOLECULAR PLANT-MICROBE INTERACTIONS 18, No. 6: 602-612. doi: 10.1094/MPMI-18-0602.
  • Haarmann T, Machado C, Lübbe Y, Correia T, Schardl CL, Panaccione DG, Tudzynski P. . ‘The ergot alkaloid gene cluster in Claviceps purpurea: extension of the cluster sequence and intra species evolution.Phytochemistry 66, No. 11: 1312-20. doi: 10.1016/j.phytochem.2005.04.011.
  • Scheffer J, Ziv C, Yarden O, Tudzynski P. . ‘The COT1 homologue CPCOT1 regulates polar growth and branching and is essential for pathogenicity in Claviceps purpurea.Fungal genetics and biology : FG & B 42, No. 2: 107-18. doi: 10.1016/j.fgb.2004.10.005.
  • Tudzynski P, Scheffer J. . ‘Claviceps purpurea: molecular aspects of a unique pathogenic lifestyle.Molecular plant pathology 5, No. 5: 377-88. doi: 10.1111/j.1364-3703.2004.00237.x.
  • Siewers V, Smedsgaard J, Tudzynski P. . ‘The P450 monooxygenase BcABA1 is essential for abscisic acid biosynthesis in Botrytis cinerea.Applied and environmental microbiology 70, No. 7: 3868-76. doi: 10.1128/AEM.70.7.3868-3876.2004.
  • Nathues E, Joshi S, Tenberge KB, von den Driesch M, Oeser B, Bäumer N, Mihlan M, Tudzynski P. . ‘CPTF1, a CREB-like transcription factor, is involved in the oxidative stress response in the phytopathogen Claviceps purpurea and modulates ROS level in its host Secale cereale.Molecular plant-microbe interactions : MPMI 17, No. 4: 383-93. doi: 10.1094/MPMI.2004.17.4.383.
  • Rolke Y, Liu S, Quidde T, Williamson B, Schouten A, Weltring KM, Siewers V, Tenberge KB, Tudzynski B, Tudzynski P. . ‘Functional analysis of H(2)O(2)-generating systems in Botrytis cinerea: the major Cu-Zn-superoxide dismutase (BCSOD1) contributes to virulence on French bean, whereas a glucose oxidase (BCGOD1) is dispensable.Molecular plant pathology 5, No. 1: 17-27. doi: 10.1111/j.1364-3703.2004.00201.x.
  • Rolke Y., Liu S., Quidde T., Williamson B., Schouten A., Weltring K.-M., Siewers V., Tenberge K. B., Tudzynski B., Tudzynski P. . ‘Functional analysis of H2O2-generating systems in Botrytis cinerea: The major Cu-Zn-superoxide dismutase (BCSOD1) contributes to virulence on French bean, whereas a glucose oxidase (BCGOD1) is dispensable.’ Molecular Plant Pathology 5, No. 1: 17-27. doi: 10.1111/j.1364-3703.2004.00201.x.
  • Correia T, Grammel N, Ortel I, Keller U, Tudzynski P. . ‘Molecular cloning and analysis of the ergopeptine assembly system in the ergot fungus Claviceps purpurea.Chemistry & biology 10, No. 12: 1281-92. doi: 10.1016/j.chembiol.2003.11.013.
  • Mey G, Correia T, Oeser B, Kershaw MJ, Garre V, Arntz C, Talbot NJ, Tudzynski P. . ‘Structural and functional analysis of an oligomeric hydrophobin gene from Claviceps purpurea.Molecular plant pathology 4, No. 1: 31-41. doi: 10.1046/j.1364-3703.2003.00138.x.
  • Mey G, Held K, Scheffer J, Tenberge KB, Tudzynski P. . ‘CPMK2, an SLT2-homologous mitogen-activated protein (MAP) kinase, is essential for pathogenesis of Claviceps purpurea on rye: evidence for a second conserved pathogenesis-related MAP kinase cascade in phytopathogenic fungi.Molecular microbiology 46, No. 2: 305-18. doi: 10.1046/j.1365-2958.2002.03133.x.
  • Oeser B, Heidrich PM, Müller U, Tudzynski P, Tenberge KB. . ‘Polygalacturonase is a pathogenicity factor in the Claviceps purpurea/rye interaction.Fungal genetics and biology : FG & B 36, No. 3: 176-86. doi: 10.1016/S1087-1845(02)00020-8.
  • Tudzynski P, Correia T, Keller U. . ‘Biotechnology and genetics of ergot alkaloids.Applied microbiology and biotechnology 57, No. 5-6: 593-605. doi: 10.1007/s002530100801.
  • Gronover CS, Kasulke D, Tudzynski P, Tudzynski B. . ‘The role of G protein alpha subunits in the infection process of the gray mold fungus Botrytis cinerea.Molecular plant-microbe interactions : MPMI 14, No. 11: 1293-302. doi: 10.1094/MPMI.2001.14.11.1293.
  • Tudzynski P, Hölter K, Correia T, Arntz C, Grammel N, Keller U. . ‘Evidence for an ergot alkaloid gene cluster in Claviceps purpurea.Molecular & general genetics : MGG 261, No. 1: 133-41.
  • Tenberge K. B., Brockmann B., Tudzynski P. . ‘Immunogold localization of an extracellular β-1,3-glucanase of the ergot fungus Claviceps purpurea during infection of rye.’ Mycological Research 103, No. 9: 1103-1118. doi: 10.1017/S095375629800820X.
  • Garre V, Müller U, Tudzynski P. . ‘Cloning, characterization, and targeted disruption of cpcat1, coding for an in planta secreted catalase of Claviceps purpurea.Molecular plant-microbe interactions : MPMI 11, No. 8: 772-83. doi: 10.1094/MPMI.1998.11.8.772.
  • Giesbert S., Lepping H.-B., Tenberge K. B., Tudzynski P. . ‘The xylanolytic system of Claviceps purpurea: Cytological evidence for secretion of xylanases in infected rye tissue and molecular characterization of two xylanase genes.’ Phytopathology 88, No. 10: 1020-1030.
  • Arntz C, Tudzynski P. . ‘Identification of genes induced in alkaloid-producing cultures of Claviceps sp.Current genetics 31, No. 4: 357-60. doi: 10.1007/s002940050216.
  • Müller U., Tenberge K.B., Oeser B., Tudzynski P. . ‘Cel1, probably encoding a cellobiohydrolase lacking the substrate binding domain, is expressed in the initial infection phase of Claviceps purpurea on Secale cereale.’ Molecular Plant-Microbe Interactions 10, No. 2: 268-279.
  • Tenberge K. B., Homann V., Oeser B., Tudzynski P. . ‘Structure and expression of two polygalacturonase genes of Claviceps purpurea oriented in tandem and cytological evidence for pectinolytic enzyme activity during infection of rye.’ Phytopathology 86, No. 10: 1084-1097.
  • Tudzynski P, van den Broek H, van den Hondel CA. . ‘Molecular genetics of pathogenic fungi: new horizons.Trends in microbiology 2, No. 11: 429-30. doi: 10.1016/0966-842X(94)90799-4.
  • Jungehülsing U, Arntz C, Smit R, Tudzynski P. . ‘The Claviceps purpurea glyceraldehyde-3-phosphate dehydrogenase gene: cloning, characterization, and use for the improvement of a dominant selection system.Current genetics 25, No. 2: 101-6. doi: 10.1007/BF00309533.
  • Dohmen G, Tudzynski P. . ‘A DNA-polymerase-related reading frame (pol-r) in the mtDNA of Secale cereale.Current genetics 25, No. 1: 59-65. doi: 10.1007/BF00712969.
  • Oeser B, Rogmann-Backwinkel P, Tudzynski P. . ‘Interaction between mitochondrial DNA and mitochondrial plasmids in Claviceps purpurea: analysis of plasmid-homologous sequences upstream of the lrRNA-gene.Current genetics 23, No. 4: 315-22. doi: 10.1007/BF00310892.
  • Oeser B, Gessner-Ulrich K, Deing P, Tudzynski P. . ‘pClK1 and pClT5--two linear mitochondrial plasmids from unrelated Claviceps purpurea strains: a comparison.Plasmid 30, No. 3: 274-80. doi: 10.1006/plas.1993.1059.
  • Smit R, Tudzynski P. . ‘Efficient transformation of Claviceps purpurea using pyrimidine auxotrophic mutants: cloning of the OMP decarboxylase gene.Molecular & general genetics : MGG 234, No. 2: 297-305.
  • Gessner-Ulrich K, Tudzynski P. . ‘Transcripts and translation products of a mitochondrial plasmid of Claviceps purpurea.Current genetics 21, No. 3: 249-54. doi: 10.1007/BF00336849.
  • Rohe M, Schründer J, Tudzynski P, Meinhardt F. . ‘Phylogenetic relationships of linear, protein-primed replicating genomes.Current genetics 21, No. 2: 173-6. doi: 10.1007/BF00318478.
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