Academics at the Department of Biology

Oppermann-Sanio, Fred Bernd, Dr. rer. nat.
Westfälische Wilhelms-Universität Münster
Institut für Molekulare Mikrobiologie und Biotechnologie
Corrensstraße 3
D-48149 Münster

Tel: + 49 - 251 - 83 3 9823
Fax: + 49 - 251 - 83 3 8388
E-mail: opperma@uni-muenster.de
net: http://mibi1.uni-muenster.de/

Lehrschwerpunkte
Mikrobiologie
- Übungen Mikrobiologie I/II (Aufbaumodule Ökologie, Evolution, Biodiversität und Genetik, Zellbiologie, Physiologie)
- Vertiefungsmodul Phylogenie und Taxonomie der Prokaryonten
- Vertiefungsmodul Mikrobielle Stoffwechselphysiologie
- Fortgeschrittenenmodul Isolierung und Charakterisierung mikrobieller Proteine
- Forschungsmodul Technisch Relevante Biopolymere durch Fermentation von Mikroorganismen

Forschungsschwerpunkte
Biochemie und Genetik der Synthese und des Abbaus von Cyanophycin
Biochemie und Genetik des mikrobiellen Katabolismus von Acetoin

Ausgewählte Projekte
Cyanophycin is a polyamide, which is synthesized by many cyanobacteria and other bacteria as a storage compound for carbon, nitrogen and energy. Biochemical and physiological investigation of the biosynthesis of cyanophycin and the genetic characterization of cyanophycin biosynthesis genes in cyanobacteria, organotrophic microorganisms und recombinant bacterial strains is one focus of investigations in our laboratory. Employing these strains, cyanophycin production is investigated in pilot-plant bioreactors with volumes of up to 500 L.
Acetoin is a product of the fermentative metabolism of many prokaryotic and eukaryotic microorganisms including species of the genus Bacillus. Detailed knowledge about the catabolism of acetoin was obtained from our studies on a diversity of acetoin-utilizing bacteria such as Pelobacter carbinolicus, Clostridium magnum, Ralstonia eutropha, Pseudomonas putida, and Bacillus subtilis.

Publications

  • Steinle A, Oppermann-Sanio FB, Reichelt R, Steinbüchel A. . ‘Synthesis and accumulation of cyanophycin in transgenic strains of Saccharomyces cerevisiae.APPLIED AND ENVIRONMENTAL MICROBIOLOGY 74, No. 11: 3410-8. doi: 10.1128/AEM.00366-08.
  • Hoppensack A, Oppermann-Sanio FB, Steinbüchel A. . ‘Conversion of the nitrogen content in liquid manure into biomass and polyglutamic acid by a newly isolated strain of Bacillus licheniformis.FEMS microbiology letters 218, No. 1: 39-45. doi: 10.1111/j.1574-6968.2003.tb11495.x.
  • Frey KM, Oppermann-Sanio FB, Schmidt H, Steinbüchel A. . ‘Technical-scale production of cyanophycin with recombinant strains of Escherichia coli.Applied and environmental microbiology 68, No. 7: 3377-84. doi: 10.1128/AEM.68.7.3377-3384.2002.
  • Obst M, Oppermann-Sanio FB, Luftmann H, Steinbüchel A. . ‘Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI. The cphE gene from P. anguilliseptica BI encodes a cyanophycinhydrolyzing enzyme.The Journal of biological chemistry 277, No. 28: 25096-105. doi: 10.1074/jbc.M112267200.
  • Krehenbrink M, Oppermann-Sanio FB, Steinbüchel A. . ‘Evaluation of non-cyanobacterial genome sequences for occurrence of genes encoding proteins homologous to cyanophycin synthetase and cloning of an active cyanophycin synthetase from Acinetobacter sp. strain DSM 587.Archives of microbiology 177, No. 5: 371-80. doi: 10.1007/s00203-001-0396-9.
  • Hai T, Oppermann-Sanio FB, Steinbüchel A. . ‘Molecular characterization of a thermostable cyanophycin synthetase from the thermophilic cyanobacterium Synechococcus sp. strain MA19 and in vitro synthesis of cyanophycin and related polyamides.Applied and environmental microbiology 68, No. 1: 93-101. doi: 10.1128/AEM.68.1.93-101.2002.
  • Oppermann-Sanio FB, Steinbüchel A. . ‘Occurrence, functions and biosynthesis of polyamides in microorganisms and biotechnological production.Die Naturwissenschaften 89, No. 1: 11-22. doi: 10.1007/s00114-001-0280-0.
  • Aboulmagd E, Voss I, Oppermann-Sanio FB, Steinbüchel A. . ‘Heterologous expression of cyanophycin synthetase and cyanophycin synthesis in the industrial relevant bacteria Corynebacterium glutamicum and Ralstonia eutropha and in Pseudomonas putida.Biomacromolecules 2, No. 4: 1338-42. doi: 10.1021/bm010075a.
  • Aboulmagd E, Oppermann-Sanio FB, Steinbüchel A. . ‘Purification of Synechocystis sp. strain PCC6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity.Applied and environmental microbiology 67, No. 5: 2176-82. doi: 10.1128/AEM.67.5.2176-2182.2001.
  • Pötter M, Oppermann-Sanio FB, Steinbüchel A. . ‘Cultivation of bacteria producing polyamino acids with liquid manure as carbon and nitrogen source.Applied and environmental microbiology 67, No. 2: 617-22. doi: 10.1128/AEM.67.2.617-622.2001.
  • Aboulmagd E, Oppermann-Sanio FB, Steinbüchel A. . ‘Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308.Archives of microbiology 174, No. 5: 297-306. doi: 10.1007/s002030000206.
  • Hai T, Oppermann-Sanio FB, Steinbüchel A. . ‘Purification and characterization of cyanophycin and cyanophycin synthetase from the thermophilic Synechococcus sp. MA19.FEMS microbiology letters 181, No. 2: 229-36. doi: 10.1111/j.1574-6968.1999.tb08849.x.
  • Huang M, Oppermann-Sanio FB, Steinbüchel A. . ‘Biochemical and molecular characterization of the Bacillus subtilis acetoin catabolic pathway.Journal of bacteriology 181, No. 12: 3837-41.
  • Huang M, Oppermann FB, Steinbüchel A. . ‘Molecular characterization of the Pseudomonas putida 2,3-butanediol catabolic pathway.FEMS microbiology letters 124, No. 2: 141-50.
  • Krüger N, Oppermann FB, Lorenzl H, Steinbüchel A. . ‘Biochemical and molecular characterization of the Clostridium magnum acetoin dehydrogenase enzyme system.Journal of bacteriology 176, No. 12: 3614-30.
  • Oppermann FB, Steinbüchel A. . ‘Identification and molecular characterization of the aco genes encoding the Pelobacter carbinolicus acetoin dehydrogenase enzyme system.Journal of bacteriology 176, No. 2: 469-85.
  • Lorenzl H, Oppermann FB, Schmidt B, Steinbüchel A. . ‘Purification and characterization of the E1 component of the Clostridium magnum acetoin dehydrogenase enzyme system.Antonie van Leeuwenhoek 64, No. 1: 9-15. doi: 10.1007/BF00870916.
  • Oppermann FB, Schmidt B, Steinbüchel A. . ‘Purification and characterization of acetoin:2,6-dichlorophenolindophenol oxidoreductase, dihydrolipoamide dehydrogenase, and dihydrolipoamide acetyltransferase of the Pelobacter carbinolicus acetoin dehydrogenase enzyme system.Journal of bacteriology 173, No. 2: 757-67.
  • Oppermann FB, Steinbüchel A, Schlegel HG. . ‘Evidence for oxidative thiolytic cleavage of acetoin in Pelobacter carbinolicus analogous to aerobic oxidative decarboxylation of pyruvate.FEMS microbiology letters 51, No. 1: 113-8.