Academics at the Department of Biology

Wissenschaftlicher Werdegang
- Studium der Biologie, Universität Hamburg
- Promotion zum Dr. rer.nat., Universität Hamburg
- Postdoctoral Fellow, Bernhard Nocht Institut für Tropenmedizin (BNI), Hamburg
- Habilitationsstipendiatin der Deutschen Forschungsgemeinschaft
- Habilitation, Universität Hamburg
- Laborleitung, Biochemie, BNI, Hamburg
- Leiterin der selbstständigen Arbeitsgruppe "Helminthen Biochemie", BNI, Hamburg
- Professor für Zoologie, Institut für Tierphysiologie, WWU Münster

Lehrschwerpunkte
- Molekulare und biochemische Parasitologie
- Molekulare Physiologie
- Strukturbiologie

Forschungsschwerpunkte
- Fremdstoffmetabolismus
- oxidativer Stress
- Glutathion
- Redox-Homöostase
- parasitische Nematoden

Ausgewählte Projekte
(1) Oxidative stress in Caenorhabditis elegans
To elucidate the protective role of glutathione S-transferases (GSTs) in a multicellular organism, we are investigating various GST-classes fromthe model organism Caenorhabditis elegans. This model system was chosen because genetic and transgenetic techniques are well developed and the system lends itself to carry out studies on organismal level under stress conditions. Furthermore, we are testing several stressors to determine whether they can induce resistance to subsequent stress. Here we are interested in analysing whether different stressors induce cross-tolerance, being not specific to the physical nature of the stressor but promoting adaption to subsequent environmental challenges.
(2) Target identification of dormant anthelminthis candidates using the model nematode Caenorhabditis elegans
Parasitic nematodes are responsible for many of the major debilitating chronic diseases of man. Drugs that affect new molecular targets are urgently needed to improve treatment and control by killing macrofilariae. Using an optimized chemogenomics based approach, we are combining new genetic mapping strategies with RNAi-screening to identify novel drug target.
(3) Comparative investigations of lipid binding proteins of parasitic nematodes
Trichinellosis is still present in Europe, especially in Eastern Europe, where hundreds of human trichinellosis cases occur yearly. Among the allergens produced by parasitic nematodes are the Lipid Binding Proteins (LBPs). The high levels of hydrophobic binding proteins in helminths, together with their restricted lipid metabolism suggests that these proteins play an important role in metabolsm and, as such, are putative targets for chemotherapy and vaccine development. the objective of the proposal is to obtain functional, structural and imminological information about Trichinella LBPs using molecular, biochemical and structural methods.

Ausgewählte Kooperationen
- diverse AGs des Bernhard-Nocht-Institut für Tropenmedizin Hamburg
- Prof. Dr. I. Bankov, Bulgarian Academy of Sciences, Sofia, Bulgaria. Fatty acid binding proteins in parasitic nematodes.
- Prof. Dr. Ricci Department of Chemical Sciences and Technologies, University of Rome "Tor Vergata", Italy. Kinetic analysis of GSTs.
- Prof. Dr. R. Madhubala, Jarwaharlal Nehru University, New Dehli, India. Exploring novel chemotherapeutic strategy for leishmaniasis.
- Dr. M. Perbandt, Prof. Dr. C. Betzel, Deutsches ElektronenSynchrotron, Hamburg. Structure determination of antioxidant enzymes of various parasites.
- Prof. Dr. A. Torda, Bioinformatics, University of Hamburg. Computational modelling.
- Prof. Dr. P. Zimniak, University of Arkansa for Medical Sciences, USA. Glutathione S-transferases from C. elegans

Publications

  • Spiegler V., Liebau E., Hensel A. . ‘Medicinal plant extracts and plant-derived polyphenols with anthelmintic activity against intestinal nematodes.’ Natural Product Reports 34, No. 6: 627-643. doi: 10.1039/c6np00126b.
  • Valentin F., Oji V., Hausser I., Liebau E., Tarinski T., Metze D., Breitkreutz D., Traupe H., Jonca N., Terheyden P. . ‘Increased expression of caspase-1 and interleukin-18 in peeling skin disease, and a novel mutation of corneodesmosin.’ Acta Dermato-Venereologica 95, No. 8: 1019-1021. doi: 10.2340/00015555-2142.
  • Spiegler V, Sendker J, Petereit F, Liebau E, Hensel A. . ‘Bioassay-Guided Fractionation of a Leaf Extract from Combretum mucronatum with Anthelmintic Activity: Oligomeric Procyanidins as the Active Principle.’ Molecules 20, No. 8: 14810-14832. doi: 10.3390/molecules200814810.
  • Stegehake D., Kurosinski M., Schürmann S., Danie J., Lüersen K., Liebau E. . ‘Polyamine-independent expression of caenorhabditis elegans antizyme.’ Journal of Biological Chemistry 290, No. 29: 18090-18101. doi: 10.1074/jbc.M115.644385.
  • Djafsia B., Ndjonka D., Dikti J., van Hoorn S., Manchang K., Brattig N., Liebau E. . ‘Immune recognition of excretory and secretory products of the filarial nematode Onchocerca ochengi in cattle and human sera.’ Journal of Helminthology null, No. null. doi: 10.1017/S0022149X15000796. [In Press]
  • Manchang T., Ajonina-Ekoti I., Ndjonka D., Eisenbarth A., Achukwi M., Renz A., Brattig N., Liebau E., Breloer M. . ‘Immune recognition of Onchocerca volvulus proteins in the human host and animal models of onchocerciasis.’ Journal of Helminthology 89, No. 3: 375-386. doi: 10.1017/S0022149X14000224.
  • Perbandt M., Eberle R., Fischer-Riepe L., Cang H., Liebau E., Betzel C. . ‘High resolution structures of Plasmodium falciparum GST complexes provide novel insights into the dimer-tetramer transition and a novel ligand-binding site.’ Journal of Structural Biology 191, No. 3: 365-375. doi: 10.1016/j.jsb.2015.06.008.
  • Bosch S., Kronenberger T., Meissner K., Zimbres F., Stegehake D., Izui N., Schettert I., Liebau E., Wrenger C. . ‘Oxidative stress control by apicomplexan parasites.’ BioMed Research International 2015, No. null. doi: 10.1155/2015/351289.
  • Eberle R., Brattig N., Trusch M., Schlüter H., Achukwi M., Eisenbarth A., Renz A., Liebau E., Perbandt M., Betzel C. . ‘Isolation, identification and functional profile of excretory-secretory peptides from Onchocerca ochengi.’ Acta Tropica 142, No. null: 156-166. doi: 10.1016/j.actatropica.2014.11.015.
  • Agyare, C.,Spiegler, V.,Sarkodie, H.,Asase,Alex A.,Liebau, E.,Hensel, A. . ‘An ethnopharmacological survey and in vitro confirmation of the ethnopharmacological use of medicinal plants as anthelmintic remedies in the Ashanti region, in the central part of Ghana.’ Journal of Ethnopharmacology 158, No. PA: 255-263. doi: 10.1016/j.jep.2014.10.029.
  • Ndjonka D., Abladam E., Djafsia B., Ajonina-Ekoti I., Achukwi M., Liebau E. . ‘Anthelmintic activity of phenolic acids from the axlewood tree Anogeissus leiocarpus on the filarial nematode Onchocerca ochengi and drug-resistant strains of the free-living nematode Caenorhabditis elegans.’ Journal of Helminthology 88, No. 4: 481-488. doi: 10.1017/S0022149X1300045X.
  • Hartmann W., Singh N., Rathaur S., Brenz Y., Liebau E., Fleischer B., Breloer M. . ‘Immunization with Brugia malayi Hsp70 protects mice against Litomosoides sigmodontis challenge infection.’ Parasite Immunology 36, No. 4: 141-149. doi: 10.1111/pim.12093.
  • Ditgen D., Anandarajah E., Meissner K., Brattig N., Wrenger C., Liebau E. . ‘Harnessing the Helminth Secretome for Therapeutic Immunomodulators.’ BioMed Research International 2014, No. null. doi: 10.1155/2014/964350.
  • Perbandt M., Ndjonka D., Liebau E. . ‘Protective mechanisms of helminths against reactive oxygen species are highly promising drug targets.’ Current Medicinal Chemistry 21, No. 15: 1794-1808. doi: 10.2174/0929867320666131119122907.
  • Hartmann W., Brenz Y., Kingsley M., Ajonina-Ekoti I., Brattig N., Liebau E., Breloer M. . ‘Nematode-Derived Proteins Suppress Proliferation and Cytokine Production of Antigen-Specific T Cells via Induction of Cell Death.’ PLoS ONE 8, No. 6. doi: 10.1371/journal.pone.0068380.
  • Ndjonka D., Rapado L., Silber A., Liebau E., Wrenger C. . ‘Natural products as a source for treating neglected parasitic diseases.’ International Journal of Molecular Sciences 14, No. 2: 3395-3439. doi: 10.3390/ijms14023395.
  • Kurosinski M., Lüersen K., Ndjonka D., Younis A., Brattig N., Liebau E. . ‘Filarial parasites possess an antizyme but lack a functional ornithine decarboxylase.’ Acta Tropica 126, No. 3: 167-176. doi: 10.1016/j.actatropica.2013.02.010.
  • Ajonina-Ekoti I., Kurosinski M., Younis A., Ndjonka D., Tanyi M., Achukwi M., Eisenbarth A., Ajonina C., Lüersen K., Breloer M., Brattig N., Liebau E. . ‘Comparative analysis of macrophage migration inhibitory factors (MIFs) from the parasitic nematode Onchocerca volvulus and the free-living nematode Caenorhabditis elegans.’ Parasitology Research 112, No. 9: 3335-3346. doi: 10.1007/s00436-013-3513-1.
  • Hertel P., Daniel J., Stegehake D., Vaupel H., Kailayangiri S., Gruel C., Woltersdorf C., Liebau E. . ‘The ubiquitin-fold modifier 1 (Ufm1) cascade of caenorhabditis elegans.’ Journal of Biological Chemistry 288, No. 15: 10661-10671. doi: 10.1074/jbc.M113.458000.
  • Lüersen K., Stegehake D., Daniel J., Drescher M., Ajonina I., Ajonina C., Hertel P., Woltersdorf C., Liebau E. . ‘The Glutathione Reductase GSR-1 Determines Stress Tolerance and Longevity in Caenorhabditis elegans.’ PLoS ONE 8, No. 4. doi: 10.1371/journal.pone.0060731.
  • Nouir N., Eschbach M., Piédavent M., Osterloh A., Kingsley M., Erttmann K., Brattig N., Liebau E., Fleischer B., Breloer M. . ‘Vaccination with Strongyloides ratti heat shock protein 60 increases susceptibility to challenge infection by induction of Th1 response.’ Vaccine 30, No. 5: 862-871. doi: 10.1016/j.vaccine.2011.11.110.
  • Ajonina-Ekoti I., Ndjonka D., Tanyi M., Wilbertz M., Younis A., Boursou D., Kurosinski M., Eberle R., Lüersen K., Perbandt M., Breloer M., Brattig N., Liebau E. . ‘Functional characterization and immune recognition of the extracellular superoxide dismutase from the human pathogenic parasite Onchocerca volvulus (OvEC-SOD).’ Acta Tropica 124, No. 1: 15-26. doi: 10.1016/j.actatropica.2012.05.013.
  • Ndjonka D., Bergmann B., Agyare C., Zimbres F., Lüersen K., Hensel A., Wrenger C., Liebau E. . ‘In vitro activity of extracts and isolated polyphenols from West African medicinal plants against Plasmodium falciparum.’ Parasitology Research 111, No. 2: 827-834. doi: 10.1007/s00436-012-2905-y.
  • Ndjonka D., Ajonina-Ekoti I., Djafsia B., Lüersen K., Abladam E., Liebau E. . ‘Anogeissus leiocarpus extract on the parasite nematode Onchocerca ochengi and on drug resistant mutant strains of the free-living nematode Caenorhabditis elegans.’ Veterinary Parasitology 190, No. null: 136-142. doi: 10.1016/j.vetpar.2012.05.027.
  • Younis AE, Geisinger F, Ajonina-Ekoti I, Soblik H, Steen H, Mitreva M, Erttmann KD, Perbandt M, Liebau E, Brattig NW. . ‘Stage-specific excretory-secretory small heat shock proteins from the parasitic nematode Strongyloides ratti - Putative links to host's intestinal mucosal defense system.’ FEBS Journal 278, No. 18: 3319-3336. doi: 10.1111/j.1742-4658.2011.08248.x.
  • Younis AE, Soblik H, Ajonina-Ekoti I, Erttmann KD, Luersen K, Liebau E, Brattig NW. . ‘Characterization of a secreted macrophage migration inhibitory factor homologue of the parasitic nematode Strongyloides acting at the parasite-host cell interface.Microbes and infection / Institut Pasteur . doi: 10.1016/j.micinf.2011.09.006.
  • Younis AE, Geisinger F, Ajonina-Ekoti I, Soblik H, Steen H, Mitreva M, Erttmann KD, Perbandt M, Liebau E, Brattig NW. . ‘Stage-specific excretory-secretory small heat shock proteins from the parasitic nematode Strongyloides ratti--putative links to host's intestinal mucosal defense system.The FEBS journal 278, No. 18: 3319-36. doi: 10.1111/j.1742-4658.2011.08248.x.
  • Yadav M, Liebau E, Haldar C, Rathaur S. . ‘Identification of major antigenic peptide of filarial glutathione-S-transferase.Vaccine 29, No. 6: 1297-303. doi: 10.1016/j.vaccine.2010.11.078.
  • Srivastava S, Srikanth E, Liebau E, Rathaur S. . ‘Identification of Setaria cervi heat shock protein70 by mass spectrometry and its evaluation as diagnostic marker for lymphatic filariasis.’ Vaccine 28, No. 5: 1429-1436. doi: 10.1016/j.vaccine.2009.06.044.
  • Radoslavov G, Jordanova R, Teofanova D, Georgieva K, Hristov P, Salomone-Stagni M, Liebau E, Bankov I. . ‘A novel secretory poly-cysteine and histidine-tailed metalloprotein (Ts-PCHTP) from Trichinella spiralis (Nematoda).PloS one 5, No. 10: e13343. doi: 10.1371/journal.pone.0013343.
  • Janowitz T, Ajonina I, Perbandt M, Woltersdorf C, Hertel P, Liebau E, Gigengack U. . ‘The 3-ureidopropionase of Caenorhabditis elegans, an enzyme involved in pyrimidine degradation.The FEBS journal 277, No. 19: 4100-9. doi: 10.1111/j.1742-4658.2010.07805.x.
  • Janowitz T, Ajonina I, Perbandt M, Woltersdorf C, Hertel P, Liebau E, Gigengack U. . ‘The 3-ureidopropionase of Caenorhabditis elegans, an enzyme involved in pyrimidine degradation.The FEBS journal . doi: 10.1111/j.1742-4658.2010.07805.x.
  • Brattig NW, Hoerauf A, Fischer PU, Liebau E, Bandi C, Debrah A, Büttner M, Büttner DW. . ‘Immunohistological studies on neoplasms of female and male Onchocerca volvulus: filarial origin and absence of Wolbachia from tumor cells.Parasitology 137, No. 5: 841-54. doi: 10.1017/S0031182009992010.
  • Srivastava S, Srikanth E, Liebau E, Rathaur S. . ‘Identification of Setaria cervi heat shock protein 70 by mass spectrometry and its evaluation as diagnostic marker for lymphatic filariasis.Vaccine 28, No. 5: 1429-36. doi: 10.1016/j.vaccine.2009.06.044.
  • Yadav M, Singh A, Rathaur S, Liebau E. . ‘Structural modeling and simulation studies of Brugia malayi glutathione-S-transferase with compounds exhibiting antifilarial activity: implications in drug targeting and designing.Journal of molecular graphics & modelling 28, No. 5: 435-45. doi: 10.1016/j.jmgm.2009.10.003.
  • Heinick A, Urban K, Roth S, Spies D, Nunes F, Phanstiel O, Liebau E, Lüersen K. . ‘Caenorhabditis elegans P5B-type ATPase CATP-5 operates in polyamine transport and is crucial for norspermidine-mediated suppression of RNA interference.The FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24, No. 1: 206-17. doi: 10.1096/fj.09-135889.
  • Ndjonka D, Agyare C, Lüersen K, Djafsia B, Achukwi D, Nukenine EN, Hensel A, Liebau E. . ‘In vitro activity of Cameroonian and Ghanaian medicinal plants on parasitic (Onchocerca ochengi) and free-living (Caenorhabditis elegans) nematodes.Journal of Helminthology . doi: 10.1017/S0022149X10000635.
  • Jordanova R, Groves MR, Kostova E, Woltersdorf C, Liebau E, Tucker PA. . ‘Fatty acid- and retinoid-binding proteins have distinct binding pockets for the two types of cargo.The Journal of biological chemistry 284, No. 51: 35818-26. doi: 10.1074/jbc.M109.022731.
  • Liebau E, Dawood KF, Fabrini R, Fischer-Riepe L, Perbandt M, Stella L, Pedersen JZ, Bocedi A, Petrarca P, Federici G, Ricci G. . ‘Tetramerization and cooperativity in Plasmodium falciparum glutathione S-transferase are mediated by atypic loop 113-119.The Journal of biological chemistry 284, No. 33: 22133-9. doi: 10.1074/jbc.M109.015198.
  • Liebau E, Höppner J, Mühlmeister M, Burmeister C, Lüersen K, Perbandt M, Schmetz C, Büttner D, Brattig N. . ‘The secretory omega-class glutathione transferase OvGST3 from the human pathogenic parasite Onchocerca volvulus.The FEBS journal 275, No. 13: 3438-53. doi: 10.1111/j.1742-4658.2008.06494.x.
  • Perbandt M, Höppner J, Burmeister C, Lüersen K, Betzel C, Liebau E. . ‘Structure of the extracellular glutathione S-transferase OvGST1 from the human pathogenic parasite Onchocerca volvulus.Journal of molecular biology 377, No. 2: 501-11. doi: 10.1016/j.jmb.2008.01.029.
  • Burmeister C, Lüersen K, Heinick A, Hussein A, Domagalski M, Walter RD, Liebau E. . ‘Oxidative stress in Caenorhabditis elegans: protective effects of the Omega class glutathione transferase (GSTO-1).The FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22, No. 2: 343-54. doi: 10.1096/fj.06-7426com.
  • Jordanova R, Radoslavov G, Fischer P, Torda A, Lottspeich F, Boteva R, Walter RD, Bankov I, Liebau E. . ‘The highly abundant protein Ag-lbp55 from Ascaridia galli represents a novel type of lipid-binding proteins.The Journal of biological chemistry 280, No. 50: 41429-38. doi: 10.1074/jbc.M504474200.
  • Ayyadevara S, Engle MR, Singh SP, Dandapat A, Lichti CF, Benes H, Shmookler Reis RJ, Liebau E, Zimniak P. . ‘Lifespan and stress resistance of Caenorhabditis elegans are increased by expression of glutathione transferases capable of metabolizing the lipid peroxidation product 4-hydroxynonenal.Aging cell 4, No. 5: 257-71. doi: 10.1111/j.1474-9726.2005.00168.x.
  • Pillai S, Kalinna BH, Liebau E, Hartmann S, Theuring F, Lucius R. . ‘Studies on Acanthocheilonema viteae cystatin: genomic organization, promoter studies and expression in Caenorhabditis elegans.Filaria journal 4: 9. doi: 10.1186/1475-2883-4-9.
  • Liebau E, De Maria F, Burmeister C, Perbandt M, Turella P, Antonini G, Federici G, Giansanti F, Stella L, Lo Bello M, Caccuri AM, Ricci G. . ‘Cooperativity and pseudo-cooperativity in the glutathione S-transferase from Plasmodium falciparum.The Journal of biological chemistry 280, No. 28: 26121-8. doi: 10.1074/jbc.M503889200.
  • Perbandt M, Höppner J, Betzel C, Walter RD, Liebau E. . ‘Structure of the major cytosolic glutathione S-transferase from the parasitic nematode Onchocerca volvulus.The Journal of biological chemistry 280, No. 13: 12630-6. doi: 10.1074/jbc.M413551200.
  • Kühnl J, Bobik T, Procter JB, Burmeister C, Höppner J, Wilde I, Lüersen K, Torda AE, Walter RD, Liebau E. . ‘Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans.The FEBS journal 272, No. 6: 1465-77. doi: 10.1111/j.1742-4658.2005.04579.x.
  • Jordanova R, Radoslavov G, Fischer P, Liebau E, Walter RD, Bankov I, Boteva R. . ‘Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli.The FEBS journal 272, No. 1: 180-9. doi: 10.1111/j.1432-1033.2004.04398.x.
  • Abo-Dalo B, Ndjonka D, Pinnen F, Liebau E, Lüersen K. . ‘A novel member of the GCN5-related N-acetyltransferase superfamily from Caenorhabditis elegans preferentially catalyses the N-acetylation of thialysine [S-(2-aminoethyl)-L-cysteine].The Biochemical journal 384, No. Pt 1: 129-37. doi: 10.1042/BJ20040789.
  • Höppner J, Perbandt M, Betzel Ch, Walter RD, Liebau E. . ‘Crystallization of the major cytosolic glutathione S-transferase from Onchocerca volvulus.Acta crystallographica. Section D, Biological crystallography 60, No. Pt 8: 1496-7. doi: 10.1107/S090744490401460X.
  • Lüersen K, Eschbach ML, Liebau E, Walter RD. . ‘Functional GATA- and initiator-like-elements exhibit a similar arrangement in the promoters of Caenorhabditis elegans polyamine synthesis enzymes.Biological chemistry 385, No. 8: 711-21. doi: 10.1515/BC.2004.087.
  • Isermann K, Liebau E, Roeder T, Bruchhaus I. . ‘A peroxiredoxin specifically expressed in two types of pharyngeal neurons is required for normal growth and egg production in Caenorhabditis elegans.Journal of molecular biology 338, No. 4: 745-55. doi: 10.1016/j.jmb.2004.03.021.
  • Perbandt M, Burmeister C, Walter RD, Betzel C, Liebau E. . ‘Native and inhibited structure of a Mu class-related glutathione S-transferase from Plasmodium falciparum.The Journal of biological chemistry 279, No. 2: 1336-42. doi: 10.1074/jbc.M309663200.
  • Müller S, Liebau E, Walter RD, Krauth-Siegel RL. . ‘Thiol-based redox metabolism of protozoan parasites.’ Trends in Parasitology 19, No. 7: 320-328. doi: 10.1016/S1471-4922(03)00141-7.
  • Rathaur S, Fischer P, Domagalski M, Walter RD, Liebau E. . ‘Brugia malayi and Wuchereria bancrofti: Gene comparison and recombinant expression of π-class related glutathione S-transferases.’ Experimental Parasitology 103, No. 3-4: 177-181. doi: 10.1016/S0014-4894(03)00093-6.
  • Burmeister C, Perbandt M, Betzel Ch, Walter RD, Liebau E. . ‘Crystallization and preliminary X-ray diffraction studies of the glutathione S-transferase from Plasmodium falciparum.Acta crystallographica. Section D, Biological crystallography 59, No. Pt 8: 1469-71. doi: 10.1107/S0907444903011090.
  • Müller S, Liebau E, Walter RD, Krauth-Siegel RL. . ‘Thiol-based redox metabolism of protozoan parasites.Trends in parasitology 19, No. 7: 320-8. doi: 10.1016/S1471-4922(03)00141-7.
  • Sommer A, Rickert R, Fischer P, Steinhart H, Walter RD, Liebau E. . ‘A dominant role for extracellular glutathione S-transferase from Onchocerca volvulus is the production of prostaglandin D2.Infection and immunity 71, No. 6: 3603-6. doi: 10.1128/IAI.71.6.3603-3606.2003.
  • Fischer P, Bonow I, Büttner DW, Kamal IH, Liebau E. . ‘An aspartate aminotransferase of Wolbachia endobacteria from Onchocerca volvulus is recognized by IgG1 antibodies from residents of endemic areas.Parasitology research 90, No. 1: 38-47. doi: 10.1007/s00436-002-0813-2.
  • Rathaur S, Fischer P, Domagalski M, Walter RD, Liebau E. . ‘Brugia malayi and Wuchereria bancrofti: gene comparison and recombinant expression of pi-class related glutathione S-transferases.Experimental parasitology 103, No. 3-4: 177-81. doi: 10.1016/S0014-4894(03)00093-6.
  • Fischer P, Wibowo H, Pischke S, Rückert P, Liebau E, Ismid IS, Supali T. . ‘PCR-based detection and identification of the filarial parasite Brugia timori from Alor Island, Indonesia.Annals of tropical medicine and parasitology 96, No. 8: 809-21. doi: 10.1179/000349802125002239.
  • Liebau E, Bergmann B, Campbell AM, Teesdale-Spittle P, Brophy PM, Lüersen K, Walter RD. . ‘The glutathione S-transferase from Plasmodium falciparum.Molecular and biochemical parasitology 124, No. 1-2: 85-90. doi: 10.1016/S0166-6851(02)00160-3.
  • Pischke S, Büttner DW, Liebau E, Fischer P. . ‘An internal control for the detection of Onchocerca volvulus DNA by PCR-ELISA and rapid detection of specific PCR products by DNA Detection Test Strips.Tropical medicine & international health : TM & IH 7, No. 6: 526-31. doi: 10.1046/j.1365-3156.2002.00890.x.
  • Sommer A, Nimtz M, Conradt HS, Brattig N, Boettcher K, Fischer P, Walter RD, Liebau E. . ‘Structural analysis and antibody response to the extracellular glutathione S-transferases from Onchocerca volvulus.Infection and immunity 69, No. 12: 7718-28. doi: 10.1128/IAI.69.12.7718-7728.2001.
  • Campbell AM, Van Eldik R, Liebau E, Barrett J, Brophy PM, Teesdale-Spittle P, Wang MF. . ‘Towards validation of glutathione S-transferase (GST) as a filarial nematode drug target.’ Chemico-Biological Interactions 133, No. 1-3: 240-243.
  • Krause S, Sommer A, Fischer P, Brophy PM, Walter RD, Liebau E. . ‘Gene structure of the extracellular glutathione S-transferase from Onchocerca volvulus and its overexpression and promoter analysis in transgenic Caenorhabditis elegans.Molecular and biochemical parasitology 117, No. 2: 145-54. doi: 10.1016/S0166-6851(01)00342-5.
  • Campbell AM, Teesdale-Spittle PH, Barrett J, Liebau E, Jefferies JR, Brophy PM. . ‘A common class of nematode glutathione S-transferase (GST) revealed by the theoretical proteome of the model organism Caenorhabditis elegans.Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 128, No. 4: 701-8. doi: 10.1016/S1096-4959(00)00360-2.
  • Klüber S, Supali T, Williams SA, Liebau E, Fischer P. . ‘Rapid PCR-based detection of Brugia malayi DNA from blood spots by DNA Detection Test Strips.Transactions of the Royal Society of Tropical Medicine and Hygiene 95, No. 2: 169-70. doi: 10.1016/S0035-9203(01)90148-8.
  • Sommer A, Fischer P, Krause K, Boettcher K, Brophy PM, Walter RD, Liebau E. . ‘A stress-responsive glyoxalase I from the parasitic nematode Onchocerca volvulus.The Biochemical journal 353, No. Pt 3: 445-52. doi: 10.1042/0264-6021:3530445.
  • Lüersen K, Müller S, Hussein A, Liebau E, Walter RD. . ‘The gamma-glutamylcysteine synthetase of Onchocerca volvulus.Molecular and biochemical parasitology 111, No. 2: 243-51. doi: 10.1016/S0166-6851(00)00325-X.
  • Brophy PM, Campbell AM, Van Eldik AJ, Teesdale-Spittle PH, Liebau E, Wang MF. . ‘β-Carbonyl substituted glutathione conjugates as inhibitors of O. volvulus GST2.’ Bioorganic and Medicinal Chemistry Letters 10, No. 9: 979-981. doi: 10.1016/S0960-894X(00)00142-6.
  • Fakae BB, Campbell AM, Barrett J, Scott IM, Teesdale-Spittle PH, Liebau E, Brophy PM. . ‘Inhibition of glutathione S-transferases (GSTs) from parasitic nematodes by extracts from traditional Nigerian medicinal plants.Phytotherapy research : PTR 14, No. 8: 630-4. doi: 10.1002/1099-1573(200012)14:8<630::AID-PTR773>3.0.CO;2-5.
  • Lüersen K, Müller S, Hussein A, Liebau E, Walter RD. . ‘The γ-glutamylcysteine synthetase of Onchocerca volvulus.’ Molecular and Biochemical Parasitology 111, No. 2: 243-251. doi: 10.1016/S0166-6851(00)00325-X.
  • Liebau E, Eschbach M-L, Tawe W, Sommer A, Fischer P, Walter RD, Henkle-Dührsen K. . ‘Identification of a stress-responsive Onchocerca volvulus glutathione S- transferase (Ov-GST-3) by RT-PCR differential display.’ Molecular and Biochemical Parasitology 109, No. 2: 101-110. doi: 10.1016/S0166-6851(00)00232-2.
  • Liebau E, Eschbach ML, Tawe W, Sommer A, Fischer P, Walter RD, Henkle-Dührsen K. . ‘Identification of a stress-responsive Onchocerca volvulus glutathione S-transferase (Ov-GST-3) by RT-PCR differential display.Molecular and biochemical parasitology 109, No. 2: 101-10. doi: 10.1016/S0166-6851(00)00232-2.
  • Brophy PM, Campbell AM, van Eldik AJ, Teesdale-Spittle PH, Liebau E, Wang MF. . ‘Beta-carbonyl substituted glutathione conjugates as inhibitors Of O. volvulus GST2.Bioorganic & medicinal chemistry letters 10, No. 9: 979-81. doi: 10.1016/S0960-894X(00)00142-6.
  • Eckelt VH, Liebau E, Walter RD, Henkle-Dührsen K. . ‘Primary sequence and activity analyses of a catalase from Ascaris suum.Molecular and biochemical parasitology 95, No. 2: 203-14. doi: 10.1016/S0166-6851(98)00092-9.
  • Wildenburg G, Liebau E, Henkle-Dührsen K. . ‘Onchocerca volvulus: ultrastructural localization of two glutathione S-transferases.Experimental parasitology 88, No. 1: 34-42. doi: 10.1006/expr.1998.4189.
  • Brattig NW, Henkle-Dührsen KH, Hounkpatin S, Liebau E, Kruppa TF, Zipfel PF. . ‘Characterization of human immune responses to the cytosolic superoxide dismutase and glutathione S-transferase from Onchocerca volvulus.Tropical medicine & international health : TM & IH 2, No. 8: 788-98. doi: 10.1046/j.1365-3156.1997.d01-384.x.
  • Liebau E, Spillner E, Henkle-Dührsen K. . ‘pOVEX vector: prokaryotic expression and purification of onchocerciasis vaccine candidate antigens as fusion proteins with the 24 kD Onchocerca volvulus glutathione S-transferase.Tropical medicine & international health : TM & IH 2, No. 7: 691-4. doi: 10.1046/j.1365-3156.1997.d01-352.x.
  • Liebau E, Eckelt VH, Wildenburg G, Teesdale-Spittle P, Brophy PM, Walter RD, Henkle-Dührsen K. . ‘Structural and functional analysis of a glutathione S-transferase from Ascaris suum.The Biochemical journal 324 ( Pt 2): 659-66.
  • Liebau E, Wildenburg G, Brophy PM, Walter RD, Henkle-Dührsen K. . ‘Biochemical analysis, gene structure and localization of the 24 kDa glutathione S-transferase from Onchocerca volvulus.Molecular and biochemical parasitology 80, No. 1: 27-39. doi: 10.1016/0166-6851(96)02660-6.
  • Liebau E, Müller V, Lucius R, Walter RD, Henkle-Dührsen K. . ‘Molecular cloning, expression and characterization of a recombinant glutathione S-transferase from Echinococcus multilocularis.Molecular and biochemical parasitology 77, No. 1: 49-56. doi: 10.1016/0166-6851(96)02578-9.
  • Liebau E, Wildenburg G, Walter RD, Henkle-Dührsen K. . ‘A novel type of glutathione S-transferase in Onchocerca volvulus.Infection and immunity 62, No. 11: 4762-7.
  • Henkle-Dührsen K, Warnecke C, Brattig N, Liebau E, Walter RD. . ‘Characterization of enzymatically active Onchocerca volvulus Cu/Zn superoxide dismutase expressed in Escherichia coli.Molecular and biochemical parasitology 67, No. 1: 41-7. doi: 10.1016/0166-6851(94)90094-9.
  • Liebau E, Walter RD, Henkle-Dührsen K. . ‘Onchocerca volvulus: isolation and sequence of a second glutathione S-transferase cDNA.Experimental parasitology 79, No. 1: 68-71. doi: 10.1006/expr.1994.1062.
  • Liebau E, Walter RD, Henkle-Dührsen K. . ‘Isolation, sequence and expression of an Onchocerca volvulus glutathione S-transferase cDNA.Molecular and biochemical parasitology 63, No. 2: 305-9. doi: 10.1016/0166-6851(94)90067-1.
  • Liebau E, Schönberger OL, Walter RD, Henkle-Dührsen KJ. . ‘Molecular cloning and expression of a cDNA encoding glutathione S-transferase from Ascaris suum.Molecular and biochemical parasitology 63, No. 1: 167-70. doi: 10.1016/0166-6851(94)90021-3.
  • Henkle-Dührsen K., Warnecke C., Brattig N., Liebau E., Walter R. . ‘Characterization of enzymatically active Onchocerca volvulus Cu Zn superoxide dismutase expressed in Escherichia coli.’ Molecular and Biochemical Parasitology 67, No. 1: 41-47. doi: 10.1016/0166-6851(94)90094-9.
  • Liebau E., Walter R., Henkleduhrsen K. . ‘Onchocerca volvulus:- Isolation and Sequence of a Second Glutathione S-Transferase cDNA.’ Experimental Parasitology 79, No. 1: 68-71. doi: 10.1006/expr.1994.1062.
  • Henkle-Dührsen K, Liebau E, Walter RD. . ‘The Onchocerca volvulus mRNAs for a hsp70, a collagen-like protein and a ribosomal protein possess a 5' spliced leader sequence.Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft für Technische Zusammenarbeit (GTZ) 44, No. 4: 337-9.
  • Henkle KJ, Liebau E, Walter RD. . ‘Characterization of the Onchocerca volvulus superoxide dismutase gene and mRNA processing.Molecular and biochemical parasitology 58, No. 1: 173-6. doi: 10.1016/0166-6851(93)90103-5.
  • Henkle KJ, Liebau E, Müller S, Bergmann B, Walter RD. . ‘Characterization and molecular cloning of a Cu/Zn superoxide dismutase from the human parasite Onchocerca volvulus.Infection and immunity 59, No. 6: 2063-9.