Mitarbeiter im Fachbereich Biologie
| Oppermann-Sanio, Fred Bernd, Dr. rer. nat. |  |
| Westfälische Wilhelms-Universität Münster Institut für Molekulare Mikrobiologie und Biotechnologie Corrensstraße 3 D-48149 Münster Tel: + 49 - 251 - 83 3 9823 Fax: + 49 - 251 - 83 3 8388 E-mail: opperma  net: http://mibi1.uni-muenster.de/ |
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| Lehrschwerpunkte | |
| Mikrobiologie - Übungen Mikrobiologie I/II (Aufbaumodule Ökologie, Evolution, Biodiversität und Genetik, Zellbiologie, Physiologie) - Vertiefungsmodul Phylogenie und Taxonomie der Prokaryonten - Vertiefungsmodul Mikrobielle Stoffwechselphysiologie - Fortgeschrittenenmodul Isolierung und Charakterisierung mikrobieller Proteine - Forschungsmodul Technisch Relevante Biopolymere durch Fermentation von Mikroorganismen |
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| Forschungsschwerpunkte | |
| Biochemie und Genetik der Synthese und des Abbaus von Cyanophycin Biochemie und Genetik des mikrobiellen Katabolismus von Acetoin |
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| ausgewählte Projekte | |
| Cyanophycin is a polyamide, which is synthesized by many cyanobacteria and other bacteria as a storage compound for carbon, nitrogen and energy. Biochemical and physiological investigation of the biosynthesis of cyanophycin and the genetic characterization of cyanophycin biosynthesis genes in cyanobacteria, organotrophic microorganisms und recombinant bacterial strains is one focus of investigations in our laboratory. Employing these strains, cyanophycin production is investigated in pilot-plant bioreactors with volumes of up to 500 L. Acetoin is a product of the fermentative metabolism of many prokaryotic and eukaryotic microorganisms including species of the genus Bacillus. Detailed knowledge about the catabolism of acetoin was obtained from our studies on a diversity of acetoin-utilizing bacteria such as Pelobacter carbinolicus, Clostridium magnum, Ralstonia eutropha, Pseudomonas putida, and Bacillus subtilis. |
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| ausgewählte Publikationen | |
| Oppermann, Schmidt, Steinbüchel (1991) Purification and characterization of acetoin:2,6-dichlorophenolindophenol oxidoreductase, dihydrolipoamide dehydrogenase, and dihydrolipoamide acetyltransferase of the Pelobacter carbinolicus acetoin dehydrogenase enzyme system. J. Bacteriol. 173: 757-767. Oppermann, Steinbüchel (1994) Identification and molecular characterization of the aco genes encoding the Pelobacter carbinolicus acetoin dehydrogenase enzyme system. J. Bacteriol. 176: 469-485. Huang, Oppermann-Sanio, Steinbüchel (1999) Biochemical and molecular characterization of the Bacillus subtilis acetoin catabolic pathway. J. Bacteriol. 181: 3837-3841. Aboulmagd, Oppermann-Sanio, Steinbüchel (2000) Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308. Arch. Microbiol 174: 297-306. Aboulmagd, Oppermann-Sanio, Steinbüchel (2001) Purification of Synechocystis PCC6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity. Appl. Environ. Microbiol. 67: 2176-2182. Krehenbrink, Oppermann-Sanio, Steinbüchel (2002) Evaluation of non-cyanobacterial genome sequences for occurrence of genes encoding proteins homologous to cyanophycin synthetase and cloning of an active cyanophycin synthetase from Acinetobacter sp. strain DSM 587. Arch. Microbiol. 177: 371-380. Obst, Oppermann-Sanio, Luftmann, Steinbüchel (2002) Isolation of cyanophycin degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI. J. Biol. Chem. 277: 25096-25105 Oppermann-Sanio, Steinbüchel (2002) Occurrence, functions and biosynthesis of polyamides in microorganisms and biotechnological production. Naturwissenschaften 89: 11-22. Oppermann-Sanio, Steinbüchel (2003) Cyanophycin. In: Biopolymers, Polyamides and Complex Proteinaceous Materials I, Vol. 7 (edited by S. R. Fahnestock and A. Steinbüchel), Wiley-VCH, Weinheim, pp. 83-106. Steinbüchel, Oppermann-Sanio, Ewering, Pötter, Reinecke (2003) Mikrobiologisches Praktikum. Springer-Verlag, Berlin |
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