Mitarbeiter im Fachbereich Biologie
| Meinhardt, Friedhelm, Prof. Dr. rer. nat. |  |
| Westfälische Wilhelms-Universität Münster Institut für Molekulare Mikrobiologie und Biotechnologie - IMMB Corrensstr. 3 D- 48149 Münster Tel: + 49 - 251 - 83 3 9825 Fax: + 49 - 251 - 83 3 8388 E-mail: meinhar  net: http://mibi.uni-muenster.de/Biologie.IMMB.Meinhardt/Index.html | |
| wissenschaftlicher Werdegang | |
| - Studium der Biologie (Diplom): 1972 - 1977, Schwerpunkte Botanik und Mikrobiologie, Ruhr Universität Bochum - Promotion zum Dr. rer. nat.: 1980 - Wiss. Mitarbeiter und Hochschuldozent: 1980 - 1987 am Lehrstuhl für Allgemeine Botanik der Ruhr-Universität - Habilitation: 1987 - Vertretung einer Professur für Mikrobiologie: 1987 - 1988, Universität Münster - Professor für Mikrobiologie, 1988 an der WWU Münster | |
| Lehrschwerpunkte | |
| - Grundlagen der Mikrobiologie - Mikrobielle Genetik und Molekularbiologie - Biotechnologie der Bacilli und der Hefen | |
| Forschungsschwerpunkte | |
| - Mikrobielle Molekularbiologie: Cytoplasmatische Plasmide von Hefen (Killerplasmide) - Mikrobielle Genetik: Genetische Regulationsmechanismen bei Bacillus - Biotechnologie: Extrazelluläre Enzyme und Sicherheitstämme von Bacillus spec. | |
| ausgewählte Projekte | |
| Extracellular enzymes and gene regulation in Bacillus Due to their high secretion capacity representatives of the Gram. pos. bacterial genus Bacillus are well known producers of biotechnologically relevant enzymes. Our work focusses on secretion optimization in different strains, such as B. licheniformis, B. megaterium, B. amyloliquefaciens. Concerted application of molecular biological and genetical techniques give rise to enzyme producers for industrial applications. Besides optimization of secretion we focus on genetic stability and biologically safe strains. Furthermore, we are generally interested in investigating regulation mechanisms (synthesis of extracellular enzymes, SOS-response, competence) with emphasis on our model organism B. megaterium. We have recently shown, that B. megaterium possesses two functional recA-genes, a novel finding, contributing in evolutionary terms to understanding of the recombination machinery. Our work is performed as a Biotech cooperation between Indonesia and Germany (IG-Biotech), that is financially supported by the German Ministry for Education and Research (BMBF) and which has Henkel KGaA/Düsseldorf as the industrial partner. Linear plasmids – Virus like elements in yeasts In addition to cytoplasmic RNA-viruses a number of yeast genera also contain cytoplasmic dsDNA-elements known as linear plasmids. The most thoroughly characterized system is represented by the killer plasmid pair pGKL1 and pGKL2 of the dairy yeast Kluyveromyces lactis. The larger element pGKL2 encodes an autonomous, cytoplasmic gene expression system whereas the smaller pGKL1 encodes a killer toxin, that arrests target cells specifically in G1 of the cell cycle. Our current work focusses on the cytoplasmic transcription and transcript-modification machinery, which display similarities to cytoplasmic viruses. Another main focus lies on the characterization of plasmid encoded killer toxins from different yeasts. We have recently shown that a novel killer toxin from Pichia acaciae and Wingea robertsiae cause DNA-damages and apoptosis in target cells. The work is supported by the Deutsche Forschungsgemeinschaft DFG. | |
| ausgewählte Publikationen | |
Klassen R, Jablonowski D, Stark MJR, Schaffrath R, Meinhardt F (2006) Mating type locus control of killer toxins from Kluyveromyces lactis and Pichia acaciae. FEMS Yeast Res 6: 404-413 Waldeck J, Daum G, Bisping B, Meinhardt F (2006) Isolation and molecular characterization of chitinase deficient Bacillus licheniformis strains capable of deproteinization of shrimp shell waste to obtain highly viscous chitin. Appl Environ Microbiol 72: 7879-7885 Nahrstedt H, Waldeck J, Gröne M, Eichstädt R, Feesche J, Meinhardt F (2005) Strain development in Bacillus licheniformis: construction of biologically contained mutants deficient in sporulation and DNA repair. J Biotechnol 119: 245-254 Nahrstedt H, Schröder C, Meinhardt F (2005) Evidence for two recA genes mediating DNA repair in Bacillus megaterium. Microbiology 151: 775-787 For a comprehensive list of publications see: | |
| ausgewählte Kooperationen | |
Prof. Dr. Bernward Bisping, IGTW, Universität Hamburg, Germany | |
