Mitarbeiter im Fachbereich Biologie
| Kudla, Jörg, Prof. Dr. rer. nat. |  |
| Westfälische Wilhelms-Universität Münster Institut für Biologie und Biotechnologie der Pflanzen Schlossplatz 4 D-48149 Münster Tel: + 49 - 251 - 83 2 4813 Fax: + 49 - 251 - 83 2 3311 E-mail: jkudla@uni-muenster.de net: http://www.uni-muenster.de/Biologie.IBBP/agkudla/index.html |
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| wissenschaftlicher Werdegang | |
| - Studium der Biologie, Hauptfach Genetik, Martin-Luther-Universität Halle - Promotion zum Dr. rer.nat., Martin-Luther-Univestität Halle - Postdoctoral Fellow, Universität Freiburg - Postdoctoral Fellow, UC Berkeley, USA - Leiter einer selbständigen Nachwuchsgruppe, Abteilung für Molekulare Botanik, Universität Ulm - Habilitation, Univesität Ulm - Professor für Molekulkare Entwicklungsbiologie der Pflanzen, Institut für Botanik und Botanischer Garten, WWU Münster |
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| Lehrschwerpunkte | |
| - Zellbiologie - Pflanzenphysiologie und pflanzliche Entwicklungsbiologie - Molekulargenetik und Molekularbiologie |
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| Forschungsschwerpunkte | |
| - Signaltransduktion: Mechanismen und Spezifität von Kalziumsignalen; Funktion von Kalziumbindungsproteinen und Proteinkinasen - Molekulare Pflanzenphysiologie: Anpassungsreaktionen an abiotischen Stress - Molekulare Entwicklunsgbiologie: Wechselwirkungen von Lichtsignalen und Induktion generativer Entwicklung - Molekulargenetik: Regulation der RNA Prozessierung in Chloroplasten |
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| ausgewählte Projekte | |
| "Functional analysis of a calcium sensor protein/serine-threonine kinase signaling network" within the framework of the AFGN (Arabidopsis Functional Genomics Network) Calcium signals regulate a wide range of biological responses to external stimuli as well as many physiological processes in plants. The proposed project aims to continue to comprehensively explore the mechanisms of a novel calcium signaling system from Arabidopsis. This system comprises a group of protein kinases (AtCIPKs), which interact with calcineurin B-like calcium sensor proteins (AtCBLs). In general, this project is focused on the functional characterization of this CBL-CIPK interaction network and its contribution to integrate and specifically decode various calcium signals in plant cells. The proposed research for the requested funding period is focused on the following main objectives: (1) Continuation of the molecular characterization of the CBL and CIPK protein families by analyzing their expression, sub-cellular localization, interaction specificity and their biochemical properties. (2) The identification of the specific physiological processes regulated by the different calcium sensor/kinase complexes by the detailed phenotypical and molecular characterization of mutant lines, which have been isolated during the first period of funding. (3) Identification of target proteins by means of yeast interaction assays. "A systems biological approach to understtanding the control of specificity in Ca2+-signaling systems" The fact that Ca2+ is a ubiquitous intracellular regulator in signaling pathways raises the important issue of how response specificity is controlled in Ca2+-based signaling systems. We have assembled a multidisciplinary team to take a systems biological approach to investigating this issue. The work will focus on Arabidopsis guard cells as a model because it is a well-characterized system with robust and easily measured outputs. We shall investigate the role of the Ca2+ sensor (CBL)/protein kinase (CIPK) module (comprising 10 CBLs and 25 CIPKs) for interpreting and decoding stimulus induced increases in Ca2+. To do this we shall employ a combination of modeling and genetic manipulation to investigate how specific Ca2+ signals are perceived and interpreted by unique combinations of CBL/CIPK components. We shall use the results from biological experiments to inform model assembly and test hypotheses generated from modeling by experimental manipulation. The results of this work will have major implications for understanding the fundamentals of Ca2+-based signaling in all organisms. |
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| ausgewählte Publikationen | |
| Kudla J., R. Hayes, W. Gruissem (1996): Polyadenylation accelerates degradation of chloroplast mRNA. EMBO J., 15, 7137-7146. Kudla, J., Q. Xu, K. Harter, W. Gruissem, S. Luan (1999): Genes for calcineurin B-like proteins in Arabidopsis are differentially regulated by stress signals. Proc. Natl. Acad. Sci. USA, 96, 4718-4723. Shi, J., K. Kim, O. Ritz, V. Albrecht, R. Gupta, K. Harter, S. Luan, J. Kudla (1999): Novel protein kinases associated with calcineurin B-like calcium sensors in Arabidopsis. Plant Cell, 11, 2393-2406. Albrecht, V., O. Ritz, S. Linder, K. Harter, J. Kudla (2001): The NAF domain defines a novel protein-protein interaction module conserved in Ca2+ regulated kinases. EMBO J., 20, 1051-1063. Sweere, U., K. Eichenberg, J. Lohrmann, V. Mira-Rodado, I. Bäurle, J. Kudla, F. Nagy, E. Schäfer, K. Harter (2001): Interaction of the response regulator ARR4 with the photoreceptor phytochrome B in modulating red light signaling. Science, 294, 1108-1111. Walter, M, J. Kilian, J. Kudla (2002): PNPase activity determines efficiency of mRNA 3' end processing, the degradation of tRNA and the extent of polyadenylation in chloroplasts. EMBO J., 21, 6905-6914. Albrecht, V., S. Weinl, D. Blazevic, C. D'Angelo, O. Batistic, Ü. Kolukisaoglu, R. Bock, B. Schulz, K. Harter, J. Kudla (2003): The calcium sensor CBL1 integrates plant responses to abiotic stresses. Plant J., 36, 457-470. Hass, C., J. Lohrmann, V. Albrecht, U. Sweere, F. Hummel, S. Dong Yoo, I. Hwang, T. Zhu, E. Schäfer, J. Kudla, K. Harter (2004): The response regulator 2 mediates ethylene signalling and hormone signal integration in Arabidopsis. EMBO J., 23, 3290-3302. Pandey, G.K., Y.W. Cheong, K. Kim, J.J. Grant, L. Li, W. Hung, C. D'Angelo, S .Weinl, J. Kudla, S. Luan (2004): The Calcium Sensor CBL9 Modulates ABA Sensitivity and Biosynthesis in Arabidopsis, Plant Cell, 16, 1912-1924. Walter, M., C. Chaban, K. Schütze, O. Batistic, K. Weckermann, C. Näke, D. Blazevic, C. Greven, K. Schumacher, C. Oecking, K. Harter, J. Kudla (2004): Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation, Plant J., 40, 428-438. |
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| ausgewählte Kooperationen | |
| - Prof. Dr. Ralph Bock, MPI Golm, Metabolomics analyses of abiotic stress responses - Prof. Dr. Klaus Harter, Univ. Tübingen, Plant two-component signaling systems - Prof. Dr. Alistair Hetherington, Univ. Lancaster, Guard cell signaling - Prof. Dr. Sheng Luan, Univ. Berkeley, The CBL/CIPK signaling system - Prof. Dr. Christophe Maurel, INRA/CNRS Montpellier, Signaling processes in roots - Prof. Dr. Tina Romeis, Univ. Berlin, Phosphorylation cascades in calcium signaling - Prof. Dr. Julian Schroeder, Calcium signaling - Prof. Dr. Mark Tester, Univ. Adelaide, Molecular analyses of ion transport processes - Prof. Dr. Jean-Baptiste Thibaud, INRA/CNRS Montpellier, Calcium regulation of ion transport - Prof. Dr. Shaul Yalovsky, Univ. Tel Aviv, Lipid modification of signaling proteins |
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